Label-free and sensitive fluorescence detection of nucleic acid, based on combination of a graphene oxid /SYBR green I dye platform and polymerase assisted signal amplification

2012 ◽  
Author(s):  
Xiao Zhu ◽  
Da Xing
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Detection ◽  
Signal Amplification ◽  
Sybr Green ◽  
Sybr Green I ◽  
Label Free

A label-free signal amplification assay for DNA detection based on exonuclease III and nucleic acid dye SYBR Green I

Talanta ◽  
2013 ◽  
Vol 114 ◽  
pp. 49-53 ◽  
Author(s):  
Aihua Zheng ◽  
Ming Luo ◽  
Dongshan Xiang ◽  
Xia Xiang ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Dna Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Label Free ◽  
Acid Dye ◽  
Exonuclease Iii ◽  
Amplification Assay

A label-free fluorescent sensor for the detection of Pb2+ and Hg2+

2015 ◽  
Vol 7 (15) ◽  
pp. 6260-6265 ◽  
Author(s):  
Hanchu Xu ◽  
Shenshan Zhan ◽  
Dongwei Zhang ◽  
Bing Xia ◽  
Xuejia Zhan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Sensor ◽  
Sybr Green ◽  
Sybr Green I ◽  
Label Free

A fluorescent sensor for the determination of Pb2+ and Hg2+ by using SYBR Green I and an integrated functional nucleic acid.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

An enzyme-free and label-free assay for copper(ii) ion detection based on self-assembled DNA concatamers and Sybr Green I

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4737 ◽  
Author(s):  
Chenchen Ge ◽  
Junhua Chen ◽  
Wei Wu ◽  
Zhiyuan Fang ◽  
Lingbo Chen ◽  
...  
Keyword(s):  
Sybr Green ◽  
Sybr Green I ◽  
Label Free ◽  
Ion Detection ◽  
Self Assembled

Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

2011 ◽  
Vol 239-242 ◽  
pp. 934-939
Author(s):  
Hui Xu ◽  
Shuli Gao ◽  
Jian Nong Chen ◽  
Quan Wen Liu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Signal ◽  
Potassium Ions ◽  
Label Free ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

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