Nondestructive imaging of stem cell in 3D scaffold

2012 ◽  
Author(s):  
Chao-Wei Chen ◽  
Andrew B. Yeatts ◽  
John P. Fisher ◽  
Yu Chen
Keyword(s):  
Stem Cell ◽  
3D Scaffold ◽  
Nondestructive Imaging

Brain-Tumor-Regenerating 3D Scaffold-Based Primary Xenograft Models for Glioma Stem Cell Targeted Drug Screening

2018 ◽  
Vol 5 (1) ◽  
pp. 139-148 ◽  
Author(s):  
Kottarapat Jeena ◽  
Cheripelil Abraham Manju ◽  
Koythatta Meethalveedu Sajesh ◽  
G Siddaramana Gowd ◽  
Thangalazhi Balakrishnan Sivanarayanan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Tumor ◽  
Drug Screening ◽  
Glioma Stem Cell ◽  
3D Scaffold ◽  
Xenograft Models ◽  
Targeted Drug

scholarly journals Retraction of “Brain-Tumor-Regenerating 3D Scaffold-Based Primary Xenograft Models for Glioma Stem Cell Targeted Drug Screening”

2019 ◽  
Vol 5 (8) ◽  
pp. 4131-4131
Author(s):  
Kottarapat Jeena ◽  
Cheripelil Abraham Manju ◽  
Koythatta Meethalveedu Sajesh ◽  
G Siddaramana Gowd ◽  
Thangalazhi Balakrishnan Sivanarayanan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Brain Tumor ◽  
Drug Screening ◽  
Glioma Stem Cell ◽  
3D Scaffold ◽  
Xenograft Models ◽  
Targeted Drug

scholarly journals Improving the immunosuppressive potential of articular chondroprogenitors in a three-dimensional culture setting

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Guillermo Bauza ◽  
Anna Pasto ◽  
Patrick Mcculloch ◽  
David Lintner ◽  
Ava Brozovich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Adipogenic Differentiation ◽  
3D Culture ◽  
Cell Populations ◽  
Stem Cell Markers ◽  
3D Scaffold

Abstract Cartilage repair in osteoarthritic patients remains a challenge. Identifying resident or donor stem/progenitor cell populations is crucial for augmenting the low intrinsic repair potential of hyaline cartilage. Furthermore, mediating the interaction between these cells and the local immunogenic environment is thought to be critical for long term repair and regeneration. In this study we propose articular cartilage progenitor/stem cells (CPSC) as a valid alternative to bone marrow-derived mesenchymal stem cells (BMMSC) for cartilage repair strategies after trauma. Similar to BMMSC, CPSC isolated from osteoarthritic patients express stem cell markers and have chondrogenic, osteogenic, and adipogenic differentiation ability. In an in vitro 2D setting, CPSC show higher expression of SPP1 and LEP, markers of osteogenic and adipogenic differentiation, respectively. CPSC also display a higher commitment toward chondrogenesis as demonstrated by a higher expression of ACAN. BMMSC and CPSC were cultured in vitro using a previously established collagen-chondroitin sulfate 3D scaffold. The scaffold mimics the cartilage niche, allowing both cell populations to maintain their stem cell features and improve their immunosuppressive potential, demonstrated by the inhibition of activated PBMC proliferation in a co-culture setting. As a result, this study suggests articular cartilage derived-CPSC can be used as a novel tool for cellular and acellular regenerative medicine approaches for osteoarthritis (OA). In addition, the benefit of utilizing a biomimetic acellular scaffold as an advanced 3D culture system to more accurately mimic the physiological environment is demonstrated.

scholarly journals Prospect of Stem Cells in Bone Tissue Engineering: A Review

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Azizeh-Mitra Yousefi ◽  
Paul F. James ◽  
Rosa Akbarzadeh ◽  
Aswati Subramanian ◽  
Conor Flavin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Types ◽  
3D Scaffold ◽  
Stem Cell Source ◽  
Induced Pluripotent

Mesenchymal stem cells (MSCs) have been the subject of many studies in recent years, ranging from basic science that looks into MSCs properties to studies that aim for developing bioengineered tissues and organs. Adult bone marrow-derived mesenchymal stem cells (BM-MSCs) have been the focus of most studies due to the inherent potential of these cells to differentiate into various cell types. Although, the discovery of induced pluripotent stem cells (iPSCs) represents a paradigm shift in our understanding of cellular differentiation. These cells are another attractive stem cell source because of their ability to be reprogramed, allowing the generation of multiple cell types from a single cell. This paper briefly covers various types of stem cell sources that have been used for tissue engineering applications, with a focus on bone regeneration. Then, an overview of some recent studies making use of MSC-seeded 3D scaffold systems for bone tissue engineering has been presented. The emphasis has been placed on the reported scaffold properties that tend to improve MSCs adhesion, proliferation, and osteogenic differentiation outcomes.

Regulation of Stem Cell Differentiation by Control of Retinoic Acid Gradients in Hydrospun 3D Scaffold

2012 ◽  
Vol 12 (5) ◽  
pp. 598-607 ◽  
Author(s):  
Roey Tzezana ◽  
Stanislav Reznik ◽  
Jacob Blumenthal ◽  
Eyal Zussman ◽  
Shulamit Levenberg
Keyword(s):  
Stem Cell ◽  
Retinoic Acid ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
3D Scaffold

scholarly journals Novel method to produce a layered 3D scaffold for human pluripotent stem cell-derived neuronal cells

2021 ◽  
Vol 350 ◽  
pp. 109043
Author(s):  
Laura Honkamäki ◽  
Tiina Joki ◽  
Nikita A. Grigoryev ◽  
Kalle Levon ◽  
Laura Ylä-Outinen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Neuronal Cells ◽  
Human Pluripotent Stem Cell ◽  
3D Scaffold ◽  
Novel Method

scholarly journals Use of l-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering

2019 ◽  
Vol 7 (10) ◽  
pp. 4310-4324 ◽  
Author(s):  
Rasha H. Dosh ◽  
Nicola Jordan-Mahy ◽  
Christopher Sammon ◽  
Christine L. Le Maitre
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Intestinal Stem Cells ◽  
3D Scaffold ◽  
Cell Tissue

Intestinal stem cells hold great potential in tissue regeneration of the intestine, however, there are key limitations in their culture in vitro.

scholarly journals Experimental and numerical investigations of fluid flow in bioreactors for optimized in vitro stem cell loading in xenografts

2018 ◽  
Vol 4 (1) ◽  
pp. 423-427
Author(s):  
Robert Ott ◽  
Carolin Wüstenhagen ◽  
Michael Stiehm ◽  
Klaus-Peter Schmitz ◽  
Stefan Siewert ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cfd Simulation ◽  
Healing Process ◽  
Three Dimensional ◽  
Flow Conditions ◽  
Micro Computed Tomography ◽  
3D Scaffold ◽  
Numerical Investigations

AbstractIn tissue engineering and regenerative medicine mesenchymal stem cells (MSC) are widely used to replace and restore the function of dysfunctional or missing tissue. Recent studies have shown significant enhancements of the in vivo healing process following dentofacial bone augmentation procedures employing stem cell-loaded xenografts. We conducted experimental and numerical investigations in perfusion flow bioreactor-xenograft-systems to identify flow conditions as well as bioreactor design features that allow for homogeneous MSC-distribution in Geistlich Bio- Oss Block xenografts. Pressure gradient - velocity characteristics and flow distributions were investigated experimentally and numerically for two bioreactor designs at steady-state flow conditions with Reynolds numbers (Re) ranging from 0.01 ≤ Re ≤ 0.32. Distilled water at 20°C with a dynamic viscosity of 1.002 mPa∙s and a density of 998 kg/m3 was used. The geometry of the xenograft utilized in three-dimensional computational fluid dynamics (CFD) simulation was obtained by means of micro-computed tomography (μCT) at an isotropic spatial resolution of 9.5 μm. The permeability values calculated from the experimental data are in good accordance with the numerical results. The investigations showed that the increase of the inflow- and outflow-area diameter, as well as the decrease of the volumetric flow rate, result in a decreasing heterogeneity of the flow distribution within the xenograft. The calculated wall shear stress rates in the three-dimensional (3D) scaffold range from 1∙10-12Pa ≤ τ ≤ 0.2 Pa. Experimentally validated CFD simulations introduced in this study provide an applicable tool to assess optimal flow conditions for homogeneous MSC distribution in bioreactor-xenograft-systems.

In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation

2019 ◽  
Vol 14 (3) ◽  
pp. 035011 ◽  
Author(s):  
Danielle LAS Amaral ◽  
Rafaella SS Zanette ◽  
Camila G Almeida ◽  
Leonara BF Almeida ◽  
Luiz FC de Oliveira ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Barium Titanate ◽  
Stem Cell Differentiation ◽  
Human Stem Cell ◽  
3D Scaffold ◽  
In Vitro Evaluation
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