A Nanostructured Molybdenum Disulfide Film for Promoting Neural Stem Cell Neuronal Differentiation: toward a Nerve Tissue-Engineered 3D Scaffold

2017 ◽  
Vol 1 (5) ◽  
pp. 1600042 ◽  
Author(s):  
Shu Wang ◽  
Jichuan Qiu ◽  
Weibo Guo ◽  
Xin Yu ◽  
Jinhui Nie ◽  
...  
Keyword(s):  
Stem Cell ◽  
Molybdenum Disulfide ◽  
Neuronal Differentiation ◽  
Neural Stem Cell ◽  
Nerve Tissue ◽  
3D Scaffold

scholarly journals Human neural stem cell intracerebral grafts show spontaneous early neuronal differentiation after several weeks

Biomaterials ◽  
2015 ◽  
Vol 44 ◽  
pp. 143-154 ◽  
Author(s):  
Annette Tennstaedt ◽  
Markus Aswendt ◽  
Joanna Adamczak ◽  
Ursel Collienne ◽  
Marion Selt ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neuronal Differentiation ◽  
Neural Stem Cell ◽  
Human Neural Stem Cell

PAX3 inhibits β-Tubulin-III expression and neuronal differentiation of neural stem cell

2017 ◽  
Vol 485 (2) ◽  
pp. 307-311 ◽  
Author(s):  
Sixian Cao ◽  
Jinfeng Du ◽  
Yan Lv ◽  
Hengrong Lin ◽  
Zuming Mao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neuronal Differentiation ◽  
Neural Stem Cell ◽  
Β Tubulin

scholarly journals Momordica Charantia Polysaccharides Modulate the Differentiation of Neural Stem Cells via SIRT1/Β-Catenin Axis in Cerebral Ischemia/Reperfusion

2020 ◽  
Author(s):  
Zhaoli Hu ◽  
Fengying Li ◽  
Xiaoling Zhou ◽  
Feng Zhang ◽  
Linyan Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Reperfusion Injury ◽  
Neuronal Differentiation ◽  
Molecular Mechanism ◽  
Neural Stem Cell ◽  
Cell Model ◽  
Ischemia Reperfusion ◽  
Neuroprotective Effects

Abstract Background Stroke is the leading cause of long-term motor disability and cognitive impairment. Recently, neurogenesis has become an attractive strategy for the chronic recovery of stroke. It is important to understand the molecular mechanism that promotes neural stem cell (NSC) neurogenesis for future NSC-based therapies. Our previous study showed that Momordica charantia polysaccharides (MCPs) exerted neuroprotective effects on stroke via their antioxidant and anti-inflammation activities. However, it remains unknown whether MCPs promote NSC neurogenesis after cerebral ischemic/reperfusion injury (IRI). Methods We investigated MCPs’ function in differentiation of NSCs in vitro experiments. Primary NSCs and neural stem cell line C17.2 were cultured and subjected to glutamate stimulation to establish the cell model of ischemia / reperfusion injury (IRI). We evaluated the effect of MCPs on NSC differentiation in IRI cell model by Western blot and immunofluorescence staining. The SIRT1 activity of NSCs post glutamate stimulation were also evaluated by CELL SIRT1 COLORIMETRY ASSAY KIT. In addition, molecular mechanism was clarified by employing the activator and inhibitor of SIRT1. Results MCPs had no effects on the differentiation of neural stem cells under physiological conditions, while shifted NSC differentiation potential from the gliogenic to neurogenic lineage under pathological conditions. Activation of SIRT1 with MCPs was responsible for the neuronal differentiation of C17.2-NSCs. The neuronal differentiation effect of MCPs was attributed to upregulation SIRT1-mediated deacetylation of β-catenin. MCPs-induced deacetylation via SIRT1 promoted nuclear accumulation of β-catenin in NSCs. Conclusion Our findings indicate that the deacetylation of β-catenin by SIRT1 represents a critical mechanism of action of MCPs in promoting NSC neuronal differentiation. It provides an improved understanding of molecular mechanism underlying neuroprotective effects of MCPs in IRI, indicating its potential role on treating ischemic stroke especially chronic recovery.

scholarly journals Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Dalia Moore ◽  
Brittney M. Meays ◽  
Lepakshe S. V. Madduri ◽  
Farah Shahjin ◽  
Subhash Chand ◽  
...  
Keyword(s):  
Autism Spectrum Disorder ◽  
Stem Cell ◽  
Neuronal Differentiation ◽  
Neural Stem Cell ◽  
Cell Model ◽  
Critical Role ◽  
Induced Pluripotent Stem Cell ◽  
Autism Spectrum ◽  
Spectrum Disorder ◽  
Induced Pluripotent

The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell- (iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with “artificial EVs” containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development.

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