Combined nonlinear laser imaging (two-photon excitation fluorescence, second and third-harmonic generation, and fluorescence lifetime imaging microscopies) in ovarian tumors

2012 ◽  
Author(s):  
J. Adur ◽  
V. B. Pelegati ◽  
A. A. de Thomaz ◽  
F. Bottcher-Luiz ◽  
L. A. L. A. Andrade ◽  
...  
Keyword(s):  
Harmonic Generation ◽  
Fluorescence Lifetime ◽  
Third Harmonic Generation ◽  
Ovarian Tumors ◽  
Fluorescence Lifetime Imaging ◽  
Laser Imaging ◽  
Third Harmonic ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation

scholarly journals Sperm metabolism is altered during storage by female insects: evidence from two-photon autofluorescence lifetime measurements in bedbugs

2015 ◽  
Vol 12 (110) ◽  
pp. 20150609 ◽  
Author(s):  
Klaus Reinhardt ◽  
Hans Georg Breunig ◽  
Aisada Uchugonova ◽  
Karsten König
Keyword(s):  
Fluorescence Lifetime ◽  
Sperm Cells ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
The Common ◽  
The Mean ◽  
The Difference ◽  
Male Genotype

We explore the possibility of characterizing sperm cells without the need to stain them using spectral and fluorescence lifetime analyses after multi-photon excitation in an insect model. The autofluorescence emission spectrum of sperm of the common bedbug, Cimex lectularius , was consistent with the presence of flavins and NAD(P)H. The mean fluorescence lifetimes showed smaller variation in sperm extracted from the male (tau m, τ m = 1.54–1.84 ns) than in that extracted from the female sperm storage organ (tau m, τ m = 1.26–2.00 ns). The fluorescence lifetime histograms revealed four peaks. These peaks (0.18, 0.92, 2.50 and 3.80 ns) suggest the presence of NAD(P)H and flavins and show that sperm metabolism can be characterized using fluorescence lifetime imaging. The difference in fluorescence lifetime variation between the sexes is consistent with the notion that female animals alter the metabolism of sperm cells during storage. It is not consistent, however, with the idea that sperm metabolism represents a sexually selected character that provides females with information about the male genotype.

Applications of Two-Photon Excitation Fluorescence Lifetime Imaging in Tumor Diagnosis

2018 ◽  
Vol 45 (2) ◽  
pp. 0207010
Author(s):  
李慧 Li Hui ◽  
夏先园 Xia Xianyuan ◽  
陈廷爱 Chen Tingai ◽  
余佳 Yu Jia ◽  
李曦 Li Xi ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Tumor Diagnosis ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Two Photon Excitation
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