Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization

2003 ◽  
Vol 63 (1) ◽  
pp. 72-80 ◽  
Author(s):  
Ye Chen ◽  
Ammasi Periasamy
Keyword(s):  
Fluorescence Lifetime ◽  
Protein Localization ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Two-photon FRET pairs based on coumarin and DBD dyes

RSC Advances ◽  
2016 ◽  
Vol 6 (40) ◽  
pp. 33510-33513 ◽  
Author(s):  
P. Wessig ◽  
N. Behrends ◽  
M. U. Kumke ◽  
U. Eisold ◽  
T. Meiling ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Photophysical Properties ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Two Photon Excitation

Synthesis and photophysical properties of coumarin–DBD FRET pairs, which are also suitable for two-photon excitation and fluorescence-lifetime imaging microscopy.

scholarly journals Two-photon excitation fluorescence lifetime imaging microscopy: A promising diagnostic tool for digestive tract tumors

2019 ◽  
Vol 12 (05) ◽  
pp. 1930009 ◽  
Author(s):  
Hui Li ◽  
Jia Yu ◽  
Rongli Zhang ◽  
Xi Li ◽  
Wei Zheng
Keyword(s):  
Digestive Tract ◽  
Fluorescence Lifetime ◽  
Biochemical Characterization ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Label Free ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Two Photon Excitation

Digestive tract tumors account for 15% and 19.3% of the cancer incidence and deaths, respectively. Early detection of digestive tract tumors is crucial to the reduction of global cancer burden. Two-photon excitation fluorescence lifetime imaging microscopy (TP-FLIM) allows non-invasive, label-free, three-dimensional, high-resolution imaging of living tissues with not only histological but also biochemical characterization ability in both qualitative and quantitative way. Benefiting from these advantages, this technology is promising for clinical diagnosis of digestive tract tumors. In recent years, many efforts have been made in this field and some remarkable progress has been achieved. In this paper, we overview the recent progress of TP-FLIM-based researches on digestive tract tumor detection. Among them, our latest results on the gastric cancer and esophageal cancer are elaborately depicted. Finally, we outlook and discuss the potential advantages and challenges of TP-FLIM in future clinical applications.

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