Comparison of quantitative flow cytometric data provided by panels with lower and increased color number

2012 ◽  
Author(s):  
József Bocsi ◽  
Anja Mittag ◽  
Arkadiusz Pierzchalski ◽  
Adolf Baumgartner ◽  
Ingo Dähnert ◽  
...  
Keyword(s):  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Quantitative Flow

scholarly journals Models for the electronic processing of flow cytometric data at high particle rates

Cytometry ◽  
1992 ◽  
Vol 13 (2) ◽  
pp. 149-154 ◽  
Author(s):  
A. van Rotterdam ◽  
J. Keij ◽  
J. W. M. Visser
Keyword(s):  
Flow Cytometric Data ◽  
High Particle ◽  
Flow Cytometric ◽  
Electronic Processing

scholarly journals The Cytomegalovirus-Specific IL-21 ELISpot Correlates with Allograft Function of Kidney Transplant Recipients

2018 ◽  
Vol 19 (12) ◽  
pp. 3945 ◽  
Author(s):  
Monika Lindemann ◽  
Johannes Korth ◽  
Ming Sun ◽  
Shilei Xu ◽  
Christoph Struve ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Estimated Glomerular Filtration Rate ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Allograft Function ◽  
Ifn Γ ◽  
Cellular Immune

In kidney transplant recipients, the cytomegalovirus (CMV) is frequently causing infection/reactivation and can trigger allograft rejection. To assess the risk of reactivation, the cellular immune response against CMV is increasingly assessed by cellular in vitro methods, such as the interferon (IFN)-γ ELISpot. In the current study we compared the IFN-γ ELISpot with our newly established CMV-specific ELISpot assays determining IL-17A, IL-21, IL-22, granzyme B, and perforin and correlated the results with flow cytometric data and clinical parameters. In 77 kidney transplant recipients, the highest frequency was observed for CMV pp65-specific cells secreting IFN-γ, followed by cells secreting IL-21 (62.9 and 23.2 Δ spot forming cells/105 cells). We observed a positive correlation between the percentage of CMV-specific CD3+ CD4+ CD154+ cells and results of the CMV-specific IL-21 ELISpot (p = 0.002). Results of the CMV pp65-specific IL-21 ELISpot correlated negatively with kidney function (estimated glomerular filtration rate, p = 0.006) and were significantly higher in women (p = 0.005). IL-21, a cytokine involved in aging that is secreted by activated CD4+ T cells, may also impact on allograft function. Thus, the CMV-specific IL-21 ELISpot could become a new tool to assess if CMV seropositivity represents a hazard for the graft.

scholarly journals QUANTITATIVE FLOW CYTOMETRIC ANALYSIS OF A AND B ANTIGENS IN 15 PATIENTS WITH cisA2B3 BLOOD GROUP

2012 ◽  
Vol 58 (3) ◽  
pp. 448-455 ◽  
Author(s):  
Etsuko Lee ◽  
Tomomi Takimoto ◽  
Shuji Ozaki ◽  
Mayumi Matsumoto ◽  
Kyoko Takeuchi ◽  
...  
Keyword(s):  
Blood Group ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Quantitative Flow

Characterization of Staphylococcus aureus-Platelet Binding by Quantitative Flow Cytometric Analysis

1992 ◽  
Vol 166 (1) ◽  
pp. 65-73 ◽  
Author(s):  
M. R. Yeaman ◽  
P. M. Sullam ◽  
P. F. Dazin ◽  
D. C. Norman ◽  
A. S. Bayer
Keyword(s):  
Staphylococcus Aureus ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Platelet Binding ◽  
Quantitative Flow

Cytokinetic investigation of lung tumors using the anti-bromodeoxyuridine (BUDR) monoclonal antibody method: Comparison with dna flow cytometric data

1990 ◽  
Vol 45 (6) ◽  
pp. 995-1001 ◽  
Author(s):  
Laura Teodori ◽  
Maria Luisa Trinca ◽  
Wolfgang Goehde ◽  
Jorg Hemmer ◽  
Franco Salvati ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Method Comparison ◽  
Lung Tumors ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Antibody Method

scholarly journals Quantitative Flow Cytometric Analysis in Erythrodermic Cutaneous T-Cell Lymphoma: Implications for Prognosis and Staging

2013 ◽  
Vol 140 (suppl 1) ◽  
pp. A216-A216
Author(s):  
Pedro Horna ◽  
Darcie M. Deaver ◽  
Lynn C. Moscinski ◽  
Eduardo M. Sotomayor ◽  
Frank Glass ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
Flow Cytometric Analysis ◽  
T Cell Lymphoma ◽  
Cutaneous T Cell Lymphoma ◽  
Flow Cytometric ◽  
Quantitative Flow
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