An analytical approach to signal reconstruction using Gaussian approximations applied to randomly generated and flow cytometric data

2000 ◽  
Vol 48 (10) ◽  
pp. 2839-2849 ◽  
Author(s):  
M. Adjouadi ◽  
C. Reyes ◽  
P. Vidal ◽  
A.B. Barreto
Keyword(s):  
Analytical Approach ◽  
Signal Reconstruction ◽  
Flow Cytometric Data ◽  
Flow Cytometric

scholarly journals Models for the electronic processing of flow cytometric data at high particle rates

Cytometry ◽  
1992 ◽  
Vol 13 (2) ◽  
pp. 149-154 ◽  
Author(s):  
A. van Rotterdam ◽  
J. Keij ◽  
J. W. M. Visser
Keyword(s):  
Flow Cytometric Data ◽  
High Particle ◽  
Flow Cytometric ◽  
Electronic Processing

scholarly journals The Cytomegalovirus-Specific IL-21 ELISpot Correlates with Allograft Function of Kidney Transplant Recipients

2018 ◽  
Vol 19 (12) ◽  
pp. 3945 ◽  
Author(s):  
Monika Lindemann ◽  
Johannes Korth ◽  
Ming Sun ◽  
Shilei Xu ◽  
Christoph Struve ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Estimated Glomerular Filtration Rate ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Allograft Function ◽  
Ifn Γ ◽  
Cellular Immune

In kidney transplant recipients, the cytomegalovirus (CMV) is frequently causing infection/reactivation and can trigger allograft rejection. To assess the risk of reactivation, the cellular immune response against CMV is increasingly assessed by cellular in vitro methods, such as the interferon (IFN)-γ ELISpot. In the current study we compared the IFN-γ ELISpot with our newly established CMV-specific ELISpot assays determining IL-17A, IL-21, IL-22, granzyme B, and perforin and correlated the results with flow cytometric data and clinical parameters. In 77 kidney transplant recipients, the highest frequency was observed for CMV pp65-specific cells secreting IFN-γ, followed by cells secreting IL-21 (62.9 and 23.2 Δ spot forming cells/105 cells). We observed a positive correlation between the percentage of CMV-specific CD3+ CD4+ CD154+ cells and results of the CMV-specific IL-21 ELISpot (p = 0.002). Results of the CMV pp65-specific IL-21 ELISpot correlated negatively with kidney function (estimated glomerular filtration rate, p = 0.006) and were significantly higher in women (p = 0.005). IL-21, a cytokine involved in aging that is secreted by activated CD4+ T cells, may also impact on allograft function. Thus, the CMV-specific IL-21 ELISpot could become a new tool to assess if CMV seropositivity represents a hazard for the graft.

Cytokinetic investigation of lung tumors using the anti-bromodeoxyuridine (BUDR) monoclonal antibody method: Comparison with dna flow cytometric data

1990 ◽  
Vol 45 (6) ◽  
pp. 995-1001 ◽  
Author(s):  
Laura Teodori ◽  
Maria Luisa Trinca ◽  
Wolfgang Goehde ◽  
Jorg Hemmer ◽  
Franco Salvati ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Method Comparison ◽  
Lung Tumors ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Antibody Method

scholarly journals Computational Analysis of Multiparametric Flow Cytometric Data to Dissect B Cell Subsets in Vaccine Studies

2019 ◽  
Vol 97 (3) ◽  
pp. 259-267 ◽  
Author(s):  
Simone Lucchesi ◽  
Emanuele Nolfi ◽  
Elena Pettini ◽  
Gabiria Pastore ◽  
Fabio Fiorino ◽  
...  
Keyword(s):  
B Cell ◽  
Computational Analysis ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
B Cell Subsets
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