Nanoparticle labeling of mesenchymal stem cells for in vivo imaging and tracking

2011 ◽  
Author(s):  
Laura M. Ricles ◽  
Seung Yun Nam ◽  
Konstantin Sokolov ◽  
Stanislav Emelianov ◽  
Laura J. Suggs
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vivo Imaging

scholarly journals Periocular and Intra-Articular Injection of Canine Adipose-Derived Mesenchymal Stem Cells: An In Vivo Imaging and Migration Study

2012 ◽  
Vol 28 (3) ◽  
pp. 307-317 ◽  
Author(s):  
Joshua A. Wood ◽  
Dai-Jung Chung ◽  
Shin Ae Park ◽  
Allison L. Zwingenberger ◽  
Christopher M. Reilly ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vivo Imaging ◽  
Migration Study ◽  
And Migration

scholarly journals In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

2014 ◽  
Vol 9 (2) ◽  
pp. 372-376 ◽  
Author(s):  
MIAO DAI ◽  
PEIRONG XU ◽  
MIN HOU ◽  
YINCHENG TENG ◽  
QINGKAI WU
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nude Mice ◽  
In Vivo Imaging ◽  
Female Nude

scholarly journals Intravenous Administration of 99mTc-HMPAO-Labeled Human Mesenchymal Stem Cells after Stroke: In Vivo Imaging and Biodistribution

2009 ◽  
Vol 18 (12) ◽  
pp. 1369-1379 ◽  
Author(s):  
Olivier Detante ◽  
Anaïck Moisan ◽  
Julien Dimastromatteo ◽  
Marie-Jeanne Richard ◽  
Laurent Riou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intravenous Administration ◽  
In Vivo Imaging ◽  
Human Mesenchymal Stem Cells ◽  
99Mtc Hmpao

Long-term in vivo imaging of human angiogenesis: Critical role of bone marrow-derived mesenchymal stem cells for the generation of durable blood vessels

2008 ◽  
Vol 75 (3) ◽  
pp. 308-314 ◽  
Author(s):  
Laura Sanz ◽  
Patricia Santos-Valle ◽  
Vanesa Alonso-Camino ◽  
Clara Salas ◽  
Antonio Serrano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Blood Vessels ◽  
In Vivo Imaging ◽  
Critical Role

Intraarticular and intravenous administration of 99MTc-HMPAO-labeled human mesenchymal stem cells (99MTC-AH-MSCS): In vivo imaging and biodistribution

2017 ◽  
Vol 46 ◽  
pp. 36-42 ◽  
Author(s):  
Luis Meseguer-Olmo ◽  
Antonio Jesús Montellano ◽  
Teresa Martínez ◽  
Carlos M. Martínez ◽  
Beatriz Revilla-Nuin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intravenous Administration ◽  
In Vivo Imaging ◽  
Human Mesenchymal Stem Cells ◽  
99Mtc Hmpao

Autologous transplantation of mesenchymal stem cells into the portal vein of the miniature pig; a preliminary experiment for NOTES approach

2019 ◽  
Vol 98 (9) ◽  
pp. 350-355
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Portal Vein ◽  
Liver Parenchyma ◽  
Miniature Pig ◽  
Hepatic Diseases ◽  
Study Results

Introduction: There is evidence that mesenchymal stem cells (MSCs) could trans-differentiate into the liver cells in vitro and in vivo and thus may be used as an unfailing source for stem cell therapy of liver disease. Combination of MSCs (with or without their differentiation in vitro) and minimally invasive procedures as laparoscopy or Natural Orifice Transluminal Endoscopic Surgery (NOTES) represents a chance for many patients waiting for liver transplantation in vain. Methods: Over 30 millions of autologous MSCs at passage 3 were transplanted via the portal vein in an eight months old miniature pig. The deposition of transplanted cells in liver parenchyma was evaluated histologically and the trans-differential potential of CM-DiI labeled cells was assessed by expression of pig albumin using immunofluorescence. Results: Three weeks after transplantation we detected the labeled cells (solitary, small clusters) in all 10 samples (2 samples from each lobe) but no diffuse distribution in the samples. The localization of CM-DiI+ cells was predominantly observed around the portal triads. We also detected the localization of albumin signal in CM-DiI labeled cells. Conclusion: The study results showed that the autologous MSCs (without additional hepatic differentiation in vitro) transplantation through the portal vein led to successful infiltration of intact miniature pig liver parenchyma with detectable in vivo trans-differentiation. NOTES as well as other newly developed surgical approaches in combination with cell therapy seem to be very promising for the treatment of hepatic diseases in near future.

Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

2019 ◽  
Vol 70 (6) ◽  
pp. 1983-1987
Author(s):  
Cristian Trambitas ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Dorin Constantin Dorobantu ◽  
Flaviu Tabaran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Bone Repair ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Specific Protocol ◽  
Repair Mechanisms ◽  
Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

Sign in / Sign up

Export Citation Format

Share Document