Regeneration of hyaline cartilage in osteochondral lesion model using L‐lysine magnetic nanoparticles labeled mesenchymal stem cells and their in vivo imaging

2020 ◽  
Vol 14 (11) ◽  
pp. 1604-1617
Author(s):  
Ruchita Shelat ◽  
Lokesh Kumar Bhatt ◽  
Bhawan Paunipagar ◽  
Thomas Kurian ◽  
Aparna Khanna ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Nanoparticles ◽  
In Vivo Imaging ◽  
Hyaline Cartilage ◽  
Osteochondral Lesion

scholarly journals Periocular and Intra-Articular Injection of Canine Adipose-Derived Mesenchymal Stem Cells: An In Vivo Imaging and Migration Study

2012 ◽  
Vol 28 (3) ◽  
pp. 307-317 ◽  
Author(s):  
Joshua A. Wood ◽  
Dai-Jung Chung ◽  
Shin Ae Park ◽  
Allison L. Zwingenberger ◽  
Christopher M. Reilly ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vivo Imaging ◽  
Migration Study ◽  
And Migration

scholarly journals In vivo imaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury

2014 ◽  
Vol 9 (2) ◽  
pp. 372-376 ◽  
Author(s):  
MIAO DAI ◽  
PEIRONG XU ◽  
MIN HOU ◽  
YINCHENG TENG ◽  
QINGKAI WU
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nude Mice ◽  
In Vivo Imaging ◽  
Female Nude

scholarly journals Intravenous Administration of 99mTc-HMPAO-Labeled Human Mesenchymal Stem Cells after Stroke: In Vivo Imaging and Biodistribution

2009 ◽  
Vol 18 (12) ◽  
pp. 1369-1379 ◽  
Author(s):  
Olivier Detante ◽  
Anaïck Moisan ◽  
Julien Dimastromatteo ◽  
Marie-Jeanne Richard ◽  
Laurent Riou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intravenous Administration ◽  
In Vivo Imaging ◽  
Human Mesenchymal Stem Cells ◽  
99Mtc Hmpao

scholarly journals Bone mesenchymal stem cells stimulation by magnetic nanoparticles and a static magnetic field: release of exosomal miR-1260a improves osteogenesis and angiogenesis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Di Wu ◽  
Xiao Chang ◽  
Jingjing Tian ◽  
Lin Kang ◽  
Yuanhao Wu ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Nanoparticles ◽  
Bone Regeneration ◽  
Static Magnetic Field ◽  
Expression Profiles ◽  
Bone Mesenchymal Stem Cells

Abstract Background The therapeutic potential of exosomes derived from stem cells has attracted increasing interest recently, because they can exert similar paracrine functions of stem cells and overcome the limitations of stem cells transplantation. Exosomes derived from bone mesenchymal stem cells (BMSC-Exos) have been confirmed to promote osteogenesis and angiogenesis. The magnetic nanoparticles (eg. Fe3O4, γ-Fe2O3) combined with a static magnetic field (SMF) has been commonly used to increase wound healing and bone regeneration. Hence, this study aims to evaluate whether exosomes derived from BMSCs preconditioned with a low dose of Fe3O4 nanoparticles with or without the SMF, exert superior pro-osteogenic and pro-angiogenic activities in bone regeneration and the underlying mechanisms involved. Methods Two novel types of exosomes derived from preconditioned BMSCs that fabricated by regulating the contents with the stimulation of magnetic nanoparticles and/or a SMF. Then, the new exosomes were isolated by ultracentrifugation and characterized. Afterwards, we conducted in vitro experiments in which we measured osteogenic differentiation, cell proliferation, cell migration, and tube formation, then established an in vivo critical-sized calvarial defect rat model. The miRNA expression profiles were compared among the exosomes to detect the potential mechanism of improving osteogenesis and angiogenesis. At last, the function of exosomal miRNA during bone regeneration was confirmed by utilizing a series of gain- and loss-of-function experiments in vitro. Results 50 µg/mL Fe3O4 nanoparticles and a 100 mT SMF were chosen as the optimum magnetic conditions to fabricate two new exosomes, named BMSC-Fe3O4-Exos and BMSC-Fe3O4-SMF-Exos. They were both confirmed to enhance osteogenesis and angiogenesis in vitro and in vivo compared with BMSC-Exos, and BMSC-Fe3O4-SMF-Exos had the most marked effect. The promotion effect was found to be related to the highly riched miR-1260a in BMSC-Fe3O4-SMF-Exos. Furthermore, miR-1260a was verified to enhance osteogenesis and angiogenesis through inhibition of HDAC7 and COL4A2, respectively. Conclusion These results suggest that low doses of Fe3O4 nanoparticles combined with a SMF trigger exosomes to exert enhanced osteogenesis and angiogenesis and that targeting of HDAC7 and COL4A2 by exosomal miR-1260a plays a crucial role in this process. This work could provide a new protocol to promote bone regeneration for tissue engineering in the future. Graphical abstract

Long-term in vivo imaging of human angiogenesis: Critical role of bone marrow-derived mesenchymal stem cells for the generation of durable blood vessels

2008 ◽  
Vol 75 (3) ◽  
pp. 308-314 ◽  
Author(s):  
Laura Sanz ◽  
Patricia Santos-Valle ◽  
Vanesa Alonso-Camino ◽  
Clara Salas ◽  
Antonio Serrano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Blood Vessels ◽  
In Vivo Imaging ◽  
Critical Role

Intraarticular and intravenous administration of 99MTc-HMPAO-labeled human mesenchymal stem cells (99MTC-AH-MSCS): In vivo imaging and biodistribution

2017 ◽  
Vol 46 ◽  
pp. 36-42 ◽  
Author(s):  
Luis Meseguer-Olmo ◽  
Antonio Jesús Montellano ◽  
Teresa Martínez ◽  
Carlos M. Martínez ◽  
Beatriz Revilla-Nuin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intravenous Administration ◽  
In Vivo Imaging ◽  
Human Mesenchymal Stem Cells ◽  
99Mtc Hmpao

Nanoparticle labeling of mesenchymal stem cells for in vivo imaging and tracking

2011 ◽  
Author(s):  
Laura M. Ricles ◽  
Seung Yun Nam ◽  
Konstantin Sokolov ◽  
Stanislav Emelianov ◽  
Laura J. Suggs
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vivo Imaging
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