High-throughput magnetic flow sorting of human cells selected on the basis of magnetophoretic mobility

2010 ◽  
Author(s):  
Lisa M. Reece ◽  
Lehanna Sanders ◽  
David Kennedy ◽  
Byron Guernsey ◽  
Paul Todd ◽  
...  
Keyword(s):  
High Throughput ◽  
Human Cells ◽  
Magnetic Flow ◽  
Flow Sorting ◽  
Magnetophoretic Mobility

Connecting Microbial Population Genetics with Microbial Pathogenesis Engineering Microfluidic Cell Arrays for High-throughput Interrogation of Host-Pathogen Interaction

2011 ◽  
pp. 533-548
Author(s):  
Palaniappan Sethu ◽  
Kalyani Putty ◽  
Yongsheng Lian ◽  
Awdhesh Kalia
Keyword(s):  
High Throughput ◽  
Bacterial Species ◽  
Human Cells ◽  
Bacterial Strains ◽  
Cell Array ◽  
Bacterial Populations ◽  
Host Pathogen Interaction ◽  
Gradient Generator ◽  
Molecular Events ◽  
Host Pathogen

A bacterial species typically includes heterogeneous collections of genetically diverse isolates. How genetic diversity within bacterial populations influences the clinical outcome of infection remains mostly indeterminate. In part, this is due to a lack of technologies that can enable contemporaneous systems-level interrogation of host-pathogen interaction using multiple, genetically diverse bacterial strains. This chapter presents a prototype microfluidic cell array (MCA) that allows simultaneous elucidation of molecular events during infection of human cells in a semi-automated fashion. It shows that infection of human cells with up to sixteen genetically diverse bacterial isolates can be studied simultaneously. The versatility of MCAs is enhanced by incorporation of a gradient generator that allows interrogation of host-pathogen interaction under four different concentrations of any given environmental variable at the same time. Availability of high throughput MCAs should foster studies that can determine how differences in bacterial gene pools and concentration-dependent environmental variables affect the outcome of host-pathogen interaction.

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

Nature ◽  
2014 ◽  
Vol 509 (7501) ◽  
pp. 487-491 ◽  
Author(s):  
Yuexin Zhou ◽  
Shiyou Zhu ◽  
Changzu Cai ◽  
Pengfei Yuan ◽  
Chunmei Li ◽  
...  
Keyword(s):  
Functional Genomics ◽  
High Throughput ◽  
High Throughput Screening ◽  
Human Cells

scholarly journals High‐throughput mapping of origins of replication in human cells

EMBO Reports ◽  
2007 ◽  
Vol 8 (8) ◽  
pp. 770-777 ◽  
Author(s):  
Isabelle Lucas ◽  
Aparna Palakodeti ◽  
Yanwen Jiang ◽  
David J Young ◽  
Nan Jiang ◽  
...  
Keyword(s):  
High Throughput ◽  
Human Cells ◽  
Origins Of Replication

scholarly journals A high-throughput DNA FISH protocol to visualize genome regions in human cells

2021 ◽  
Vol 2 (3) ◽  
pp. 100741
Author(s):  
Elizabeth H. Finn ◽  
Tom Misteli
Keyword(s):  
High Throughput ◽  
Human Cells

scholarly journals CENP-A associated lncRNAs influence chromosome segregation in human cells

2017 ◽  
Author(s):  
Delphine Quénet ◽  
David Sturgill ◽  
Marin Olson ◽  
Yamini Dalal
Keyword(s):  
Chromosome Segregation ◽  
High Throughput ◽  
Satellite Dna ◽  
Human Cells ◽  
Histone Variant ◽  
Data Support ◽  
Dna Elements

ABSTRACTTranscription occurs ubiquitously throughout non-coding parts of the genome, including at repetitive α-satellite DNA elements which comprise the majority of human centromeres. The function of temporally regulated centromeric transcription, and transcripts, is consequently a topic of intense investigation. In this study, we use high throughput approaches to identify and describe lncRNAs associated with the centromere specific histone variant CENP-A that arise from the transcription of specific centromeres at early G1, which we then show are physically associated with centromeres, and which are functionally necessary for accurate chromosome segregation. Targeted depletion of one such centromeric RNA, which originates from a single centromere, is sufficient to increase the frequency of chromosome segregation defects. These data support the emerging paradigm of the necessity of centromere-specific lncRNAs in the integrity of faithful chromosome segregation.

scholarly journals Versatile phenotype-activated cell sorting

2020 ◽  
Vol 6 (43) ◽  
pp. eabb7438
Author(s):  
Jihwan Lee ◽  
Zhuohe Liu ◽  
Peter H. Suzuki ◽  
John F. Ahrens ◽  
Shujuan Lai ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Sorting ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Human Cells ◽  
Target Cells ◽  
Temporal Properties ◽  
Single Target ◽  
New Variant ◽  
Improved Methods

Unraveling the genetic and epigenetic determinants of phenotypes is critical for understanding and re-engineering biology and would benefit from improved methods to separate cells based on phenotypes. Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or human cells with unique spatiotemporal profiles from heterogeneous populations. SPOTlight relies on imaging visual phenotypes by microscopy, precise optical tagging of single target cells, and retrieval of tagged cells by fluorescence-activated cell sorting. To illustrate SPOTlight’s ability to screen cells based on temporal properties, we chose to develop a photostable yellow fluorescent protein for extended imaging experiments. We screened 3 million cells expressing mutagenesis libraries and identified a bright new variant, mGold, that is the most photostable yellow fluorescent protein reported to date. We anticipate that the versatility of SPOTlight will facilitate its deployment to decipher the rules of life, understand diseases, and engineer new molecules and cells.

Sign in / Sign up

Export Citation Format

Share Document