scholarly journals Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds

2010 ◽  
Author(s):  
Darcy S. Peterka ◽  
Volodymyr Nikolenko ◽  
Elodie Fino ◽  
Roberto Araya ◽  
Roberto Etchenique ◽  
...  
Keyword(s):  
Spatial Light Modulator ◽  
Light Modulator ◽  
Two Photon ◽  
Ruthenium Compounds ◽  
Neuronal Imaging ◽  
And Control

scholarly journals High Fidelity Spatial Light Modulator Configuration for Photo-Stimulation

2021 ◽  
Vol 9 ◽  
Author(s):  
Samira Aghayee ◽  
Mitchell Weikert ◽  
Phillip Alvarez ◽  
Gabriel A. Frank ◽  
Wolfgang Losert
Keyword(s):  
Spatial Light Modulator ◽  
Signal To Noise Ratio ◽  
Spatial Light Modulators ◽  
High Signal ◽  
High Fidelity ◽  
Light Modulator ◽  
Fourier Plane ◽  
Two Photon ◽  
Light Modulators ◽  
The Brain

For their capacity to shape optical wavefronts in real time into any desired illumination pattern, phase-only Spatial Light Modulators (SLM) have proven to be powerful tools for optical trapping and micromanipulation applications. SLMs are also becoming increasingly utilized in selective photo-stimulation of groups of neurons in the brain. However, conventional SLM based wavefront modulation introduces artifacts that are particularly detrimental for photo-stimulation applications. The primary issue is the unmodulated light that travels along the 0th order of diffraction. This portion of light is commonly blocked at the center of the object plane, which prevents photo-stimulation in the blocked region. We demonstrate a virtual lens configuration that moves the 1st order diffraction with the desired illumination pattern into the Fourier plane of the 0th order light. This virtual lens setup makes the whole field of view accessible for photo-stimulation and eliminates the need for removing the 0th order light in two-photon applications. Furthermore, in an example application to reconstruct a pattern consisting of an array of points, the virtual lens configuration increases the uniformity of the intensities these points. Moreover, diffraction-induced artifacts are also significantly reduced within the target plane. Therefore, our proposed high fidelity configuration yields target points with high signal to noise ratio.

scholarly journals Precise, 3-D optogenetic control of the diameter of single arterioles

2021 ◽  
Author(s):  
Philip J. O’Herron ◽  
David A. Hartmann ◽  
Kun Xie ◽  
Prakash Kara ◽  
Andy Y. Shih
Keyword(s):  
Blood Flow ◽  
Neural Activity ◽  
Light Modulator ◽  
Spatiotemporal Resolution ◽  
Two Photon ◽  
Light Pulses ◽  
All Optical ◽  
Health And Disease ◽  
And Control

AbstractModulation of brain arteriole diameter is critical for maintenance of cerebral blood pressure and control of hyperemia during regional neural activity. However, studies of hemodynamic function in health and disease have lacked a method to control and monitor blood flow with high spatiotemporal resolution. Here, we describe a new all-optical approach to precisely control and monitor arteriolar contractility in vivo using combined two-photon optogenetics and imaging. The expression of the excitatory opsin, ReaChR, in vascular smooth muscle cells enabled rapid and repeated vasoconstriction following brief light pulses. Targeted two-photon activation of ReaCHR using a spatial light modulator (SLM) produced highly localized constrictions when targeted to individual arteries within the neocortex. We demonstrate the utility of this method for examining arteriole contractile dynamics and creating transient blood flow reductions. Additionally, we show that optogenetic constriction can offset or completely block sensory stimulus evoked vasodilation, providing a valuable tool to dissociate blood flow changes from neural activity.

Femtosecond Two-Photon 3D Lightfield Lithography

2021 ◽  
Author(s):  
Aravind Jakkinapalli ◽  
Balaji Baskar ◽  
Sy-Bor Wen
Keyword(s):  
Spatial Light Modulator ◽  
Light Field ◽  
Single Photon ◽  
Microlens Array ◽  
Laser Pulses ◽  
Femtosecond Laser Pulses ◽  
Virtual Image ◽  
Light Modulator ◽  
Two Photon ◽  
Uv Led

Abstract Based on the successful single-photon 3D light field photolithography we demonstrated in the last year, we extend the methodology to femtosecond 3D light field lithography. Compared with our previous single-photon work with UV LED light, using femtosecond light and the associated two-photon light absorption in 3D light field lithography can cure photoresist only around designed voxel locations in a 3D space. Such a two-photon scheme can prevent the unwilling curing of photoresists along the optical paths of rays before arriving at designed voxel locations, which is observed in our previous UV LED-based single-photon 3D light field lithography. The experimental scheme of femtosecond two-photon 3D light field lithography starts from delivering uniform femtosecond laser pulses to a spatial light modulator. The designed pixel map is presented on the spatial light modulator and then delivered to a microlens array to construct a 3D virtual image in the free space. By compressing the 3D virtual image in a photoresist layer with a microscope system, we can successfully generate different microscale 3D patterns without relying on scanning processes as in traditional 3D lithography. In this study, we present preliminary results of (a) algorithms developed to generated 3D patterns with femtosecond light, which should satisfy additional constraints when femtosecond light is used, and (b) 3D patterns generated in photoresists with femtosecond two-photon 3D light field lithography.

scholarly journals Image processing and control of a programmable spatial light modulator

2011 ◽  
Author(s):  
Abdul A. S. Awwal ◽  
Richard Leach ◽  
Gordon Brunton ◽  
Eddy Tse ◽  
JoAnn Matone ◽  
...  
Keyword(s):  
Image Processing ◽  
Spatial Light Modulator ◽  
Light Modulator ◽  
And Control

Two-photon excitation microscopy with spatial light modulator

2017 ◽  
Author(s):  
Naoya Matsumoto ◽  
Alu Konno ◽  
Takashi Inoue ◽  
Haruyoshi Toyoda ◽  
Toshiyuki Miwa ◽  
...  
Keyword(s):  
Spatial Light Modulator ◽  
Light Modulator ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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