Two-photon multifocal structured illumination microscopy based on a high-speed phase only spatial light modulator

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Keyword(s):  
High Speed ◽  
Spatial Light Modulator ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Modulator ◽  
Two Photon

Live Cell Imaging With Spatial Light Modulator-based Optical Sectioning Structured Illumination Microscopy

2014 ◽  
Vol 20 (S3) ◽  
pp. 388-389
Author(s):  
Zdeněk Švindrych ◽  
Pavel Křížek ◽  
Evgeny Smirnov ◽  
Martin Ovesný ◽  
Josef Borkovec ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Spatial Light Modulator ◽  
Cell Imaging ◽  
Live Cell ◽  
Structured Illumination ◽  
Optical Sectioning ◽  
Structured Illumination Microscopy ◽  
Light Modulator

scholarly journals mmSIM: an open toolbox for accessible structured illumination microscopy

2021 ◽  
Vol 379 (2199) ◽  
Author(s):  
Craig T. Russell ◽  
Michael Shaw
Keyword(s):  
Open Source ◽  
Open Source Software ◽  
High Speed ◽  
Super Resolution ◽  
Software Tools ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Modulator ◽  
Wide Range ◽  
Hardware Designs

Since the first practical super-resolution structured illumination fluorescence microscopes (SIM) were demonstrated more than two decades ago, the method has become increasingly popular for a wide range of bioimaging applications. The high cost and relative inflexibility of commercial systems, coupled with the conceptual simplicity of the approach and the desire to exploit and customize existing hardware, have led to the development of a large number of home-built systems. Several detailed hardware designs are available in the scientific literature, complemented by open-source software tools for SIM image validation and reconstruction. However, there remains a lack of simple open-source software to control these systems and manage the synchronization between hardware components, which is critical for effective SIM imaging. This article describes a new suite of software tools based on the popular Micro-Manager package, which enable the keen microscopist to develop and run a SIM system. We use the software to control two custom-built, high-speed, spatial light modulator-based SIM systems, evaluating their performance by imaging a range of fluorescent samples. By simplifying the process of SIM hardware development, we aim to support wider adoption of the technique. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

scholarly journals Isotropic image in structured illumination microscopy patterned with a spatial light modulator

2009 ◽  
Vol 17 (17) ◽  
pp. 14710 ◽  
Author(s):  
Bo-Jui Chang ◽  
Li-Jun Chou ◽  
Yun-Ching Chang ◽  
Su-Yu Chiang
Keyword(s):  
Spatial Light Modulator ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Modulator

scholarly journals mmSIM: An open toolbox for accessible structured illumination microscopy

2021 ◽  
Author(s):  
Craig T. Russell ◽  
Michael Shaw
Keyword(s):  
Open Source ◽  
Open Source Software ◽  
High Speed ◽  
Super Resolution ◽  
Software Tools ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Modulator ◽  
Wide Range ◽  
Hardware Designs

SummarySince the first practical super-resolution structured illumination fluorescence microscopes (SIM) were demonstrated more than two decades ago the method has become increasingly popular for a wide range of bioimaging applications. The high cost and relative inflexibility of commercial systems, coupled with the conceptual simplicity of the approach and the desire to exploit and customise existing hardware, have led to the development of a large number of home-built systems. Several detailed hardware designs are available in the scientific literature, complemented by open-source software tools for SIM image validation and reconstruction. However, there remains a lack of simple open-source software to control these systems and manage the synchronization between hardware components, which is critical for effective SIM imaging. This article describes a new suite of software tools based on the popular Micro-Manager package, which enable the keen microscopist to develop and run a SIM system. We use the software to control two custom-built, high-speed, spatial light modulator-based SIM systems, evaluating their performance by imaging a range of fluorescent samples. By simplifying the process of SIM hardware development, we aim to support wider adoption of the technique.

scholarly journals Efficient Multifocal Structured Illumination Microscopy Utilizing a Spatial Light Modulator

2020 ◽  
Vol 10 (12) ◽  
pp. 4396
Author(s):  
Liang Feng ◽  
Xiaolei Wang ◽  
Xinlei Sun ◽  
Sende Wang ◽  
Lie Lin ◽  
...  
Keyword(s):  
Focal Plane ◽  
Spatial Light Modulator ◽  
Present System ◽  
Wide Field ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Modulator ◽  
Reconstruction Procedure ◽  
Efficient System ◽  
Phase Plates

We demonstrated an efficient system for multifocal structured illumination microscopy (MSIM) utilizing a spatial light modulator (SLM). Nine phase profiles of chessboard phase plates loaded on the SLM in sequence are used to generate nine multifocal arrays on the focal plane. Subsequently, nine raw multifocal images are acquired. Finally, by extracting the parameters of the illumination patterns from the raw images precisely, a final super-resolved image is reconstructed by performing the standard reconstruction procedure of structured illumination microscopy (SIM). Our MSIM system realized nearly a 1.5-fold enhancement in spatial resolution compared with wide-field (WF) microscopy. The feasibility of the present system is validated on experiments and the results show its great performances along with good compatibility.

High-speed programmable lithium niobate thin film spatial light modulator

2021 ◽  
Vol 46 (5) ◽  
pp. 1037
Author(s):  
Xuanchao Ye ◽  
Fengchao Ni ◽  
Honggen Li ◽  
Haigang Liu ◽  
Yuanlin Zheng ◽  
...  
Keyword(s):  
Thin Film ◽  
Lithium Niobate ◽  
High Speed ◽  
Spatial Light Modulator ◽  
Light Modulator

High-Speed Structured Illumination Microscopy and Its Applications

2020 ◽  
Vol 57 (24) ◽  
pp. 240001
Author(s):  
赵天宇 Zhao Tianyu ◽  
汪召军 Wang Zhaojun ◽  
冯坤 Feng Kun ◽  
梁言生 Liang Yansheng ◽  
何旻儒 He Minru ◽  
...  
Keyword(s):  
High Speed ◽  
Structured Illumination ◽  
Structured Illumination Microscopy
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