Multiparametric optimization of multispectral optoacoustic tomography for deep tissue imaging

2010 ◽  
Author(s):  
Jürgen Glatz ◽  
Nikolaos C. Deliolanis ◽  
Lu Ding ◽  
Adrian Taruttis ◽  
Amir Rosenthal ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Optoacoustic Tomography ◽  
Multiparametric Optimization ◽  
Deep Tissue Imaging

scholarly journals Optoaccoustic Tomography – good news for microcirculatory research

2022 ◽  
Vol 18 (2) ◽  
pp. 1-13
Author(s):  
Tiago Granja ◽  
Sérgio Faloni de Andrade ◽  
Luis Monteiro Rodrigues
Keyword(s):  
Current Knowledge ◽  
Good News ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Healthy Human ◽  
Optoacoustic Tomography ◽  
Multiple Wavelength ◽  
Human Volunteers ◽  
Optimal Resolution ◽  
Deep Tissue Imaging

Tomographic imaging is a well established technology in preventive medicine and biomedical research, although not without limitations and concerns. Optoacoustic tomography (OAT) is a recent development that bridges optical and sonographic techniques to solve spatial resolution in deep-tissue imaging. In addition to safety advantages, OAT allows multiple wavelength readings for natural thermoelastic chromophores. In this study, we explore Multi Spectral Optoacoustic Tomography (MSOT) capacities to simultaneously acquire three independent chromophores – deoxygenated haemoglobin (Hb), oxygenated haemoglobin (HbO2), and melanin, from healthy human volunteers, with maximal molar extinction of HbO2 at 950 nm, Hb at 750 nm and melanin at 680 nm. Later we demonstrate how image stability during acquisition is fundamental for optimal resolution, precision and consistency of high throughout MSOT data collection. From recorded scans, a workflow is layered for data evaluation. With the MSOT dedicated software results were extracted from 3D image analysis of deep (15 mm3) vessels. The possibilities offered by this new system, specially in vascular pathophysiology, are immense and can be extended beyond current knowledge.

scholarly journals Optoaccoustic Tomography – good news for microcirculatory research

2022 ◽  
Vol 18 (2) ◽  
pp. 1
Author(s):  
Tiago Granja ◽  
Sérgio Faloni de Andrade ◽  
Luis Monteiro Rodrigues
Keyword(s):  
Current Knowledge ◽  
Good News ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Healthy Human ◽  
Optoacoustic Tomography ◽  
Multiple Wavelength ◽  
Human Volunteers ◽  
Optimal Resolution ◽  
Deep Tissue Imaging

Tomographic imaging is a well established technology in preventive medicine and biomedical research, although not without limitations and concerns. Optoacoustic tomography (OAT) is a recent development that bridges optical and sonographic techniques to solve spatial resolution in deep-tissue imaging. In addition to safety advantages, OAT allows multiple wavelength readings for natural thermoelastic chromophores. In this study, we explore Multi Spectral Optoacoustic Tomography (MSOT) capacities to simultaneously acquire three independent chromophores – deoxygenated haemoglobin (Hb), oxygenated haemoglobin (HbO2), and melanin, from healthy human volunteers, with maximal molar extinction of HbO2 at 950 nm, Hb at 750 nm and melanin at 680 nm. Later we demonstrate how image stability during acquisition is fundamental for optimal resolution, precision and consistency of high throughout MSOT data collection. From recorded scans, a workflow is layered for data evaluation. With the MSOT dedicated software results were extracted from 3D image analysis of deep (15 mm3) vessels. The possibilities offered by this new system, specially in vascular pathophysiology, are immense and can be extended beyond current knowledge.

Responsive optical probes for deep-tissue imaging: Photoacoustics and second near-infrared fluorescence

2021 ◽  
Vol 173 ◽  
pp. 141-163
Author(s):  
Fei Ding ◽  
Jing Feng ◽  
Xueli Zhang ◽  
Jielin Sun ◽  
Chunhai Fan ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Optical Probes ◽  
Near Infrared Fluorescence ◽  
Deep Tissue Imaging

scholarly journals Slow light for deep tissue imaging with ultrasound modulation

2012 ◽  
Vol 100 (13) ◽  
pp. 131102 ◽  
Author(s):  
Huiliang Zhang ◽  
Mahmood Sabooni ◽  
Lars Rippe ◽  
Chulhong Kim ◽  
Stefan Kröll ◽  
...  
Keyword(s):  
Slow Light ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Ultrasound Modulation

scholarly journals Transgenic mice: beyond the knockout

2011 ◽  
Vol 300 (2) ◽  
pp. F291-F300 ◽  
Author(s):  
R. Lance Miller
Keyword(s):  
Transgenic Mice ◽  
Fluorescent Proteins ◽  
Primary Cultures ◽  
Cell Lineage ◽  
Tissue Imaging ◽  
Cell Type ◽  
Deep Tissue ◽  
Pure Cell ◽  
Deep Tissue Imaging ◽  
Tremendous Impact

Transgenic mice have had a tremendous impact on biomedical research. Most researchers are familiar with transgenic mice that carry Cre recombinase (Cre) and how they are used to create conditional knockouts. However, some researchers are less familiar with many of the other types of transgenic mice and their applications. For example, transgenic mice can be used to study biochemical and molecular pathways in primary cultures and cell suspensions derived from transgenic mice, cell-cell interactions using multiple fluorescent proteins in the same mouse, and the cell cycle in real time and in the whole animal, and they can be used to perform deep tissue imaging in the whole animal, follow cell lineage during development and disease, and isolate large quantities of a pure cell type directly from organs. These novel transgenic mice and their applications provide the means for studying of molecular and biochemical events in the whole animal that was previously limited to cell cultures. In conclusion, transgenic mice are not just for generating knockouts.

Designing Upconversion Nanocrystals Capable of 745 nm Sensitization and 803 nm Emission for Deep-Tissue Imaging

2016 ◽  
Vol 22 (31) ◽  
pp. 10801-10807 ◽  
Author(s):  
Liangliang Liang ◽  
Xiaoji Xie ◽  
Daniel Teh Boon Loong ◽  
Angelo Homayoun All ◽  
Ling Huang ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Upconversion Nanocrystals ◽  
Deep Tissue Imaging
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