Nonlinear optical properties of photoswitchable fluorescent proteins

2009 ◽  
Author(s):  
Inge Asselberghs ◽  
Cristina Flors ◽  
Evelien De Meulenaere ◽  
Benoît Champagne ◽  
Jos Vanderleyden ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Fluorescent Proteins ◽  
Nonlinear Optical Properties

Second-order nonlinear optical properties of fluorescent proteins for second-harmonic imaging

2009 ◽  
Vol 19 (40) ◽  
pp. 7514 ◽  
Author(s):  
Evelien De Meulenaere ◽  
Inge Asselberghs ◽  
Marc de Wergifosse ◽  
Edith Botek ◽  
Stijn Spaepen ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Fluorescent Proteins ◽  
Second Harmonic ◽  
Harmonic Imaging ◽  
Second Order ◽  
Nonlinear Optical Properties

NONLINEAR OPTICAL PROPERTIES OF mSTRAWBERRY AND mCHERRY FOR SECOND HARMONIC IMAGING

2010 ◽  
Vol 19 (01) ◽  
pp. 1-13 ◽  
Author(s):  
EVELIEN DE MEULENAERE ◽  
MARC DE WERGIFOSSE ◽  
EDITH BOTEK ◽  
STIJN SPAEPEN ◽  
BENOÎT CHAMPAGNE ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Rayleigh Scattering ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Second Harmonic ◽  
Harmonic Signal ◽  
Harmonic Imaging ◽  
Nonlinear Optical Properties ◽  
Lower Energy Band

The second-order nonlinear optical properties of two monomeric red fluorescent proteins, mStrawberry and mCherry, have been experimentally determined by frequency-resolved femtosecond hyper-Rayleigh scattering. These proteins were found to exhibit a stronger nonlinear response than the previously described eGFP, eYFP and DsRed,1 confirming the trend that fluorophores with a more extended conjugated system, or a lower-energy band gap between ground and excited state, exhibit a larger static hyperpolarizability (β0). Furthermore, these experimental data were complemented with quantum chemical calculations. A discrepancy was observed between experimental and theoretical results, but this could be explained by the chromophore model extracted from the available X-ray diffraction data. While eGFP showed a larger dynamic experimental response (βHRS) due to the highest resonance enhancement, we measured an even higher signal for mCherry. Furthermore, mCherry also shows a better separation of the second harmonic signal and two-photon excited fluorescent signal, making this the preferred fluorescent protein for second harmonic imaging at 800 nm so far.

MAGNETIC FIELD, PRESSURE AND TEMPERATURE DEPENDENT OPTICAL PROPERTIES IN A GaAs 9.0 P 1.0 QUANTUM DOT

2014 ◽  
Vol 6 (2) ◽  
pp. 1178-1190
Author(s):  
A. JOHN PETER ◽  
Ada Vinolin
Keyword(s):  
Magnetic Field ◽  
Optical Properties ◽  
Quantum Dot ◽  
Field Strength ◽  
Magnetic Field Strength ◽  
Photon Energy ◽  
Nonlinear Optical ◽  
Binding Energies ◽  
Nonlinear Optical Properties ◽  
Optical Rectification

Simultaneous effects of magnetic field, pressure and temperature on the exciton binding energies are found in a 9.0 1.0 6.0 4.0 GaAs P / GaAs P quantum dot. Numerical calculations are carried out taking into consideration of spatial confinement effect. The cylindrical system is taken in the present problem with the strain effects. The electronic properties and the optical properties are found with the combined effects of magnetic field strength, hydrostatic pressure and temperature values. The exciton binding energies and the nonlinear optical properties are carried out taking into consideration of geometrical confinement and the external perturbations.Compact density approach is employed to obtain the nonlinear optical properties. The optical rectification coefficient is obtained with the photon energy in the presence of pressure, temperature and external magnetic field strength. Pressure and temperature dependence on nonlinear optical susceptibilities of generation of second and third order harmonics as a function of incident photon energy are brought out in the influence of magnetic field strength. The result shows that the electronic and nonlinear optical properties are significantly modified by the applications of external perturbations in a 9.0 1.0 6.0 4.0 GaAs P / GaAs P quantum dot.

Linear and Nonlinear Optical Properties of Small Silicon Clusters

1990 ◽  
Author(s):  
Tapio T. Rantala ◽  
Mark I. Stockman ◽  
Daniel A. Jelski ◽  
Thomas F. George
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Nonlinear Optical Properties ◽  
Silicon Clusters ◽  
Linear And Nonlinear
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