Second-order nonlinear optical properties of fluorescent proteins for second-harmonic imaging

2009 ◽  
Vol 19 (40) ◽  
pp. 7514 ◽  
Author(s):  
Evelien De Meulenaere ◽  
Inge Asselberghs ◽  
Marc de Wergifosse ◽  
Edith Botek ◽  
Stijn Spaepen ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Fluorescent Proteins ◽  
Second Harmonic ◽  
Harmonic Imaging ◽  
Second Order ◽  
Nonlinear Optical Properties

NONLINEAR OPTICAL PROPERTIES OF mSTRAWBERRY AND mCHERRY FOR SECOND HARMONIC IMAGING

2010 ◽  
Vol 19 (01) ◽  
pp. 1-13 ◽  
Author(s):  
EVELIEN DE MEULENAERE ◽  
MARC DE WERGIFOSSE ◽  
EDITH BOTEK ◽  
STIJN SPAEPEN ◽  
BENOÎT CHAMPAGNE ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Rayleigh Scattering ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Second Harmonic ◽  
Harmonic Signal ◽  
Harmonic Imaging ◽  
Nonlinear Optical Properties ◽  
Lower Energy Band

The second-order nonlinear optical properties of two monomeric red fluorescent proteins, mStrawberry and mCherry, have been experimentally determined by frequency-resolved femtosecond hyper-Rayleigh scattering. These proteins were found to exhibit a stronger nonlinear response than the previously described eGFP, eYFP and DsRed,1 confirming the trend that fluorophores with a more extended conjugated system, or a lower-energy band gap between ground and excited state, exhibit a larger static hyperpolarizability (β0). Furthermore, these experimental data were complemented with quantum chemical calculations. A discrepancy was observed between experimental and theoretical results, but this could be explained by the chromophore model extracted from the available X-ray diffraction data. While eGFP showed a larger dynamic experimental response (βHRS) due to the highest resonance enhancement, we measured an even higher signal for mCherry. Furthermore, mCherry also shows a better separation of the second harmonic signal and two-photon excited fluorescent signal, making this the preferred fluorescent protein for second harmonic imaging at 800 nm so far.

Synthesis and Second-Order Nonlinear Optical Properties of New Coumaromethacrylate-Methylmethacrylate Copolymers

1989 ◽  
Vol 173 ◽  
Author(s):  
R. A. Henry ◽  
J. M. Hoover ◽  
A. Knoesen ◽  
S. T. Kowel ◽  
G. A. Lindsay ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Elevated Temperatures ◽  
Second Harmonic ◽  
Second Order ◽  
Nonlinear Optical Properties ◽  
Glass Transitions ◽  
Copolymer Films ◽  
New Family ◽  
Polymerizable Group

ABSTRACTThe synthesis and second-order nonlinear optical (NLO) properties of a new family of optically nonlinear coumarin dye-containing polymers is reported. Polymerizable coumaromethacrylate (CMA) monomers having various alkyl spacers (C2 to C5) between the polymerizable group and the chromophore were prepared and copolymerized with methylmethacrylate (MMA) and other vinyl monomers. The CMA copolymers had high chromophoric content (up to 18 × 1020) and glass transitions as high as 132°C. Spin cast films were prepared on BK7 substrates and oriented by corona-onset poling at elevated temperatures (COPET). The poled CMA copolymer films were characterized for spectroscopic absorption and second harmonic properties. A comparison of the nonlinear optical properties of poled CMA-MMA copolymers to poled guest-host systems is made. Relative to guest/host systems, CMA-MMA copolymer films exhibit larger and much more stable second-order nonlinear optical properties.

SECOND-ORDER NONLINEAR OPTICAL PROPERTIES OF CHROMOPHORE-COATED PARTICLES: SYMMETRY CONSIDERATIONS

2007 ◽  
Vol 16 (01) ◽  
pp. 27-35 ◽  
Author(s):  
KASPER BAERT ◽  
KURT WOSTYN ◽  
RENAUD A. L. VALLÉE ◽  
KOEN CLAYS
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Rayleigh Scattering ◽  
Second Harmonic ◽  
Nonlinear Effects ◽  
Second Order ◽  
Nonlinear Optical Properties ◽  
Harmonic Intensity ◽  
Nonlinear Optical Chromophores ◽  
Optical Chromophores

The second-order nonlinear optical properties of polystyrene particles coated with two well-characterized nonlinear optical chromophores with different molecular symmetry have been studied. From the markedly different depolarization ratio for the hyper-Rayleigh scattering from the molecular scatterers and from the largely different second-harmonic intensity from particles coated with these chromophores, we conjecture that contributions from antisymmetric molecular electronic states are necessary to observe second-order nonlinear effects from centrosymmetrical particles in isotropic solution.

LINEAR AND SECOND-ORDER NONLINEAR OPTICAL PROPERTIES OF ARRAYS OF NONCENTROSYMMETRIC GOLD NANOPARTICLES

2002 ◽  
Vol 11 (04) ◽  
pp. 421-432 ◽  
Author(s):  
HEMMO TUOVINEN ◽  
MARTTI KAURANEN ◽  
KONSTANTINS JEFIMOVS ◽  
PASI VAHIMAA ◽  
THOMAS VALLIUS ◽  
...  
Keyword(s):  
Optical Properties ◽  
Electron Beam Lithography ◽  
Nonlinear Optical ◽  
Electromagnetic Interaction ◽  
Second Harmonic ◽  
Second Order ◽  
Nonlinear Optical Properties ◽  
Metal Particles ◽  
Gold Particles ◽  
Plasmon Resonances

The optical properties of nanoscopic arrays of metal particles are dominated by plasmon resonances and electromagnetic interaction between the particles. We use electron-beam lithography to prepare arrays of noncentrosymmetric gold particles and study their linear and second-order nonlinear optical properties. By varying the orientation of the particles in a fixed lattice, we observe shifts in the polarized linear extinction spectra. The second-harmonic generation efficiencies of the two types of samples differ by up to 60%. The results show that the properties of the samples are sensitive to the smallest details of their structure.

Second harmonic generation of a series of axially substituted titanium(IV) and gallium(III) tetra-tert-butylphthalocyanines

2003 ◽  
Vol 07 (04) ◽  
pp. 291-295 ◽  
Author(s):  
Christian G. Claessens ◽  
Andreas Gouloumis ◽  
Markus Barthel ◽  
Yu Chen ◽  
Guillermo Martin ◽  
...  
Keyword(s):  
Optical Properties ◽  
Electric Field ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Second Order ◽  
Nonlinear Optical Properties ◽  
Phthalocyanine Ring

Electric Field Induced Second-Harmonic (EFISH) Generation and Hyper Raleigh Scattering (HRS) measurements were performed on a representative series of axially substituted titanium(IV) and gallium(III) tetra-tert-butylphthalocyanines. The comparison of γEFISH and βHRS values within the series and in particular in the case of the dimeric species shows the influence of both the reduction of symmetry of the phthalocyanine ring and the intramolecular stacking on the second order nonlinear optical properties.

Second-order nonlinear optical properties of two chalcone derivatives: insights from sum-over-states

2021 ◽  
Author(s):  
Jean Custodio ◽  
Giulio Demetrius Creazo d'Oliveira ◽  
Fernando Gotardo ◽  
Leandro Cocca ◽  
Leonardo De Boni ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Theoretical Study ◽  
Second Order ◽  
Nonlinear Optical Properties ◽  
Chalcone Derivatives

In the following study, a combined experimental and theoretical study of the nonlinear optical properties (NLO) of two chalcone derivatives, (E)-3-(2-methoxyphenyl)-1-(2-(phenylsulfonylamine)phenyl)prop-2-en-1-one (MPSP) and (E)-3-(3-nitrophenyl)-1-(2-(phenylsulfonylamine)phenyl)prop-2-en-1-one (NPSP) in DMSO is reported. Single...

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