The measurement of red blood cell volume change induced by Ca2+based on full field quantitative phase microscopy

2009 ◽  
Author(s):  
Seungrag Lee ◽  
Ji Yong Lee ◽  
Wenzhong Yang ◽  
Dug Young Kim
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
Volume Change ◽  
Red Blood Cell ◽  
Full Field ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy

scholarly journals Imaging red blood cell dynamics by quantitative phase microscopy

2008 ◽  
Vol 41 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Gabriel Popescu ◽  
YoungKeun Park ◽  
Wonshik Choi ◽  
Ramachandra R. Dasari ◽  
Michael S. Feld ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Dynamics ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy

Red blood cell three-dimensional morphometry by quantitative phase microscopy

2015 ◽  
Author(s):  
F. Merola ◽  
P. Memmolo ◽  
L. Miccio ◽  
O. Gennari ◽  
M. Mugnano ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Three Dimensional ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy

scholarly journals Characterization of Liposomes Using Quantitative Phase Microscopy (QPM)

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Jennifer Cauzzo ◽  
Nikhil Jayakumar ◽  
Balpreet Singh Ahluwalia ◽  
Azeem Ahmad ◽  
Nataša Škalko-Basnet
Keyword(s):  
Rapid Development ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Batch Mode ◽  
Full Field ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Tracking Ability

The rapid development of nanomedicine and drug delivery systems calls for new and effective characterization techniques that can accurately characterize both the properties and the behavior of nanosystems. Standard methods such as dynamic light scattering (DLS) and fluorescent-based assays present challenges in terms of system’s instability, machine sensitivity, and loss of tracking ability, among others. In this study, we explore some of the downsides of batch-mode analyses and fluorescent labeling, while introducing quantitative phase microscopy (QPM) as a label-free complimentary characterization technique. Liposomes were used as a model nanocarrier for their therapeutic relevance and structural versatility. A successful immobilization of liposomes in a non-dried setup allowed for static imaging conditions in an off-axis phase microscope. Image reconstruction was then performed with a phase-shifting algorithm providing high spatial resolution. Our results show the potential of QPM to localize subdiffraction-limited liposomes, estimate their size, and track their integrity over time. Moreover, QPM full-field-of-view images enable the estimation of a single-particle-based size distribution, providing an alternative to the batch mode approach. QPM thus overcomes some of the drawbacks of the conventional methods, serving as a relevant complimentary technique in the characterization of nanosystems.

scholarly journals In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm

2016 ◽  
Vol 34 (5) ◽  
pp. 211-214 ◽  
Author(s):  
Mustafa S. Al Musawi ◽  
Mohamad Suhaimi Jafar ◽  
Bassam T. Al-Gailani ◽  
Naser Mahmoud Ahmed ◽  
Fatanah Mohamad Suhaimi ◽  
...  
Keyword(s):  
Solid State ◽  
Blood Cell ◽  
Cell Volume ◽  
Volume Change ◽  
Red Blood Cell ◽  
Diode Pump ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Full-field and single-shot quantitative phase microscopy using dynamic speckle illumination

2011 ◽  
Vol 36 (13) ◽  
pp. 2465 ◽  
Author(s):  
Youngwoon Choi ◽  
Taeseok Daniel Yang ◽  
Kyoung Jin Lee ◽  
Wonshik Choi
Keyword(s):  
Single Shot ◽  
Full Field ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Dynamic Speckle

scholarly journals Single Cell Volume Measurement by Quantitative Phase Microscopy (QPM): A Case Study of Erythrocyte Morphology

2006 ◽  
Vol 17 (5-6) ◽  
pp. 193-200 ◽  
Author(s):  
Claire L. Curl ◽  
Catherine J. Bellair ◽  
Peter J. Harris ◽  
Brendan E. Allman ◽  
Ann Roberts ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Volume ◽  
Volume Measurement ◽  
Erythrocyte Morphology ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy
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