scholarly journals Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals

2008 ◽  
Author(s):  
Jae Youn Hwang ◽  
Hasmik Agadjanian ◽  
Lali K. Medina-Kauwe ◽  
Zeev Gross ◽  
Harry B. Gray ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Field Of View ◽  
Large Field ◽  
Fluorescence Lifetime Imaging ◽  
Small Animals ◽  
Lifetime Imaging

Fluorescence Lifetime Imaging and Fourier Transform Infrared Spectroscopy of Michelangelo's David

2005 ◽  
Vol 59 (9) ◽  
pp. 1174-1181 ◽  
Author(s):  
Daniela Comelli ◽  
Gianluca Valentini ◽  
Rinaldo Cubeddu ◽  
Lucia Toniolo
Keyword(s):  
Fourier Transform ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Inorganic Compounds ◽  
Fluorescence Emission ◽  
Chemical Characterization ◽  
Fourier Transform Infrared ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Analytical Measurements

We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.

Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

2002 ◽  
Author(s):  
Michael Wahl ◽  
Hans-Juergen Rahn ◽  
Uwe Ortmann ◽  
Rainer Erdmann ◽  
Martin Boehmer ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

2007 ◽  
Vol 6 (5) ◽  
pp. 7290.2007.00030 ◽  
Author(s):  
Abedelnasser Abulrob ◽  
Eric Brunette ◽  
Jacqueline Slinn ◽  
Ewa Baumann ◽  
Danica Stanimirovic
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Time Domain ◽  
Ex Vivo ◽  
Renal Ischemia ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Lifetime Analysis

Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment. Therefore, fluorescence lifetime imaging could potentially be applied to in vivo diagnostic assessment of changes in the tissue microenvironment caused by disease, such as ischemia. The aim of this study was to evaluate the utility of noninvasive fluorescence lifetime imaging in distinguishing between normal and ischemic kidney tissue in vivo. Mice were subjected to 60-minute unilateral kidney ischemia followed by 6-hour reperfusion. Animals were then injected with the near-infrared fluorescence probe Cy5.5 or saline and imaged using a time-domain small-animal optical imaging system. Both fluorescence intensity and lifetime were acquired. The fluorescence intensity of Cy5.5 was clearly reduced in the ischemic compared with the contralateral kidney, and the fluorescence lifetime of Cy5.5 was not detected in the ischemic kidney, suggesting reduced kidney clearance. Interestingly, the two-component lifetime analysis of endogenous fluorescence at 700 nm distinguished renal ischemia in vivo without the need for Cy5.5 injection for contrast enhancement. The average fluorescence lifetime of endogenous tissue fluorophores was a sensitive indicator of kidney ischemia ex vivo. The study suggests that fluorescence lifetime analysis of endogenous tissue fluorophores could be used to discriminate ischemic or necrotic tissues by noninvasive in vivo or ex vivo organ imaging.

All-solid-state diode-pumped fluorescence lifetime imaging system for biomedicine and microscopy

1999 ◽  
Author(s):  
M. J. Cole ◽  
K. Dowling ◽  
R. Jones ◽  
D. Parsons-Karavassilis ◽  
P. M. W. French ◽  
...  
Keyword(s):  
Solid State ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Diode Pumped

scholarly journals Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

2015 ◽  
Vol 40 (14) ◽  
pp. 3296 ◽  
Author(s):  
Ximeng Y. Dow ◽  
Shane Z. Sullivan ◽  
Ryan D. Muir ◽  
Garth J. Simpson
Keyword(s):  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging

A Two-Dimensional Fluorescence Lifetime Imaging System Using a Gated Image Intensifier

1991 ◽  
Vol 45 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Xue Feng Wang ◽  
Teruo Uchida ◽  
David M. Coleman ◽  
Shigeo Minami
Keyword(s):  
Fluorescence Lifetime ◽  
Imaging System ◽  
Image Intensifier ◽  
Fluorescence Lifetime Imaging ◽  
Two Dimensional ◽  
Lifetime Imaging
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