Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

2002 ◽  
Author(s):  
Michael Wahl ◽  
Hans-Juergen Rahn ◽  
Uwe Ortmann ◽  
Rainer Erdmann ◽  
Martin Boehmer ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Fluorescence Lifetime Imaging and Fourier Transform Infrared Spectroscopy of Michelangelo's David

2005 ◽  
Vol 59 (9) ◽  
pp. 1174-1181 ◽  
Author(s):  
Daniela Comelli ◽  
Gianluca Valentini ◽  
Rinaldo Cubeddu ◽  
Lucia Toniolo
Keyword(s):  
Fourier Transform ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Inorganic Compounds ◽  
Fluorescence Emission ◽  
Chemical Characterization ◽  
Fourier Transform Infrared ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Analytical Measurements

We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.

All-solid-state diode-pumped fluorescence lifetime imaging system for biomedicine and microscopy

1999 ◽  
Author(s):  
M. J. Cole ◽  
K. Dowling ◽  
R. Jones ◽  
D. Parsons-Karavassilis ◽  
P. M. W. French ◽  
...  
Keyword(s):  
Solid State ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Diode Pumped

scholarly journals Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

2015 ◽  
Vol 40 (14) ◽  
pp. 3296 ◽  
Author(s):  
Ximeng Y. Dow ◽  
Shane Z. Sullivan ◽  
Ryan D. Muir ◽  
Garth J. Simpson
Keyword(s):  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging

scholarly journals Targetable Conformationally Restricted Cyanines Enable Photon-Count Limited Applications

2021 ◽  
Author(s):  
Patrick Eiring ◽  
Ryan McLaughlin ◽  
Siddharth Matikonda ◽  
HAN ZHONGYING ◽  
Lennart Grabenhorst ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Ring System ◽  
Fluorescence Lifetime Imaging ◽  
Conformationally Restricted ◽  
Lifetime Imaging ◽  
Tertiary Amide ◽  
Imaging Performance

Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications. We recently demonstrated that appending a ring system to the pentamethine cyanine ring system improves the quantum yield and extends the fluorescence lifetime. Here, we report an optimized synthesis of persulfonated variants that enable efficient labeling of nucleic acids and proteins. We demonstrate that a bifunctional sulfonated tertiary amide significantly improves the optical properties of the resulting bioconjugates. These new conformationally restricted cyanines are compared to parent species in a range of contexts including their use on a DNA-nano-antenna, in single-molecule Förster resonance energy transfer (FRET) applications, far-red fluorescence lifetime imaging microscopy (FLIM), and single-molecule localization microscopy. These efforts define contexts in which eliminating cyanine isomerization provides meaningful benefits to imaging performance.

Simultaneous single molecule atomic force and fluorescence lifetime imaging

2010 ◽  
Author(s):  
Olaf Schulz ◽  
Felix Koberling ◽  
Deron Walters ◽  
Marcelle Koenig ◽  
Jacob Viani ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Atomic Force

A Two-Dimensional Fluorescence Lifetime Imaging System Using a Gated Image Intensifier

1991 ◽  
Vol 45 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Xue Feng Wang ◽  
Teruo Uchida ◽  
David M. Coleman ◽  
Shigeo Minami
Keyword(s):  
Fluorescence Lifetime ◽  
Imaging System ◽  
Image Intensifier ◽  
Fluorescence Lifetime Imaging ◽  
Two Dimensional ◽  
Lifetime Imaging
Sign in / Sign up

Export Citation Format

Share Document