Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

2008 ◽  
Author(s):  
Igor L. Medintz ◽  
Thomas Pons ◽  
James B. Delehanty ◽  
Kimihiro Susumu ◽  
Philip E. Dawson ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Fluorescent Protein ◽  
Intracellular Delivery

scholarly journals Self-Assembled Quantum Dot−Peptide Bioconjugates for Selective Intracellular Delivery

2006 ◽  
Vol 17 (4) ◽  
pp. 920-927 ◽  
Author(s):  
James B. Delehanty ◽  
Igor L. Medintz ◽  
Thomas Pons ◽  
Florence M. Brunel ◽  
Philip E. Dawson ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Intracellular Delivery ◽  
Self Assembled

Intracellular delivery of and sensing with quantum dot bioconjugates

2009 ◽  
Author(s):  
James B. Delehanty ◽  
Christopher E. Bradburne ◽  
Igor L. Medintz ◽  
Dorothy Farrell ◽  
Thomas Pons ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Intracellular Delivery

scholarly journals Quantum Dot–Fluorescent Protein FRET Probes for Sensing Intracellular pH

ACS Nano ◽  
2012 ◽  
Vol 6 (4) ◽  
pp. 2917-2924 ◽  
Author(s):  
Allison M. Dennis ◽  
Won Jong Rhee ◽  
David Sotto ◽  
Steven N. Dublin ◽  
Gang Bao
Keyword(s):  
Quantum Dot ◽  
Intracellular Ph ◽  
Fluorescent Protein

scholarly journals Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells

2020 ◽  
Vol 117 (20) ◽  
pp. 10976-10982 ◽  
Author(s):  
Jason N. Belling ◽  
Liv K. Heidenreich ◽  
Zhenhua Tian ◽  
Alexandra M. Mendoza ◽  
Tzu-Ting Chiou ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Acoustic Waves ◽  
Fluorescent Protein ◽  
Single Channel ◽  
Intracellular Delivery ◽  
Cell Types ◽  
Membrane Repair ◽  
Hematopoietic Stem ◽  
Cell Therapies ◽  
Membrane Rupture

Advances in gene editing are leading to new medical interventions where patients’ own cells are used for stem cell therapies and immunotherapies. One of the key limitations to translating these treatments to the clinic is the need for scalable technologies for engineering cells efficiently and safely. Toward this goal, microfluidic strategies to induce membrane pores and permeability have emerged as promising techniques to deliver biomolecular cargo into cells. As these technologies continue to mature, there is a need to achieve efficient, safe, nontoxic, fast, and economical processing of clinically relevant cell types. We demonstrate an acoustofluidic sonoporation method to deliver plasmids to immortalized and primary human cell types, based on pore formation and permeabilization of cell membranes with acoustic waves. This acoustofluidic-mediated approach achieves fast and efficient intracellular delivery of an enhanced green fluorescent protein-expressing plasmid to cells at a scalable throughput of 200,000 cells/min in a single channel. Analyses of intracellular delivery and nuclear membrane rupture revealed mechanisms underlying acoustofluidic delivery and successful gene expression. Our studies show that acoustofluidic technologies are promising platforms for gene delivery and a useful tool for investigating membrane repair.

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