Quantifying local heterogeneity of in vivo transport dynamics using stochastic scanning multiphoton multifocal microscopy and segmented spatiotemporal image correlation spectroscopy

2008 ◽  
Author(s):  
Hee Y. Kim ◽  
Justin E. Jureller ◽  
Andrey Kuznetsov ◽  
Louis H. Philipson ◽  
Norbert F. Scherer
Keyword(s):  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Transport Dynamics ◽  
Local Heterogeneity ◽  
Image Correlation Spectroscopy ◽  
Spatiotemporal Image Correlation

Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

2006 ◽  
Vol 84 (6) ◽  
pp. 825-831 ◽  
Author(s):  
Ian R. Bates ◽  
Paul W. Wiseman ◽  
John W. Hanrahan
Keyword(s):  
Membrane Proteins ◽  
Fluorescence Correlation Spectroscopy ◽  
Fluorescence Recovery After Photobleaching ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Fluorescence Correlation ◽  
Transport Dynamics ◽  
Health And Disease ◽  
Image Correlation Spectroscopy ◽  
Selection Of

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

Easy Monitoring of Velocity Fields in Microfluidic Devices Using Spatiotemporal Image Correlation Spectroscopy

2013 ◽  
Vol 85 (17) ◽  
pp. 8080-8084 ◽  
Author(s):  
Marco Travagliati ◽  
Salvatore Girardo ◽  
Dario Pisignano ◽  
Fabio Beltram ◽  
Marco Cecchini
Keyword(s):  
Microfluidic Devices ◽  
Velocity Fields ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Image Correlation Spectroscopy ◽  
Spatiotemporal Image Correlation

Exploring oligomeric state of the serotonin1A receptor utilizing photobleaching image correlation spectroscopy: implications for receptor function

2018 ◽  
Vol 207 ◽  
pp. 409-421 ◽  
Author(s):  
Hirak Chakraborty ◽  
Md. Jafurulla ◽  
Andrew H. A. Clayton ◽  
Amitabha Chattopadhyay
Keyword(s):  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Membrane Cholesterol ◽  
Receptor Function ◽  
Oligomeric State ◽  
Serotonin1a Receptor ◽  
Image Correlation Spectroscopy

Photobleaching image correlation spectroscopy (pbICS) reveals that membrane cholesterol modulates the oligomeric state of the serotonin1A receptor.

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