scholarly journals Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching

2008 ◽  
Vol 147 (4) ◽  
pp. 1646-1658 ◽  
Author(s):  
Jérôme Bove ◽  
Benoit Vaillancourt ◽  
Jens Kroeger ◽  
Peter K. Hepler ◽  
Paul W. Wiseman ◽  
...  
Keyword(s):  
Fluorescence Recovery After Photobleaching ◽  
Pollen Tubes ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Fluorescence Recovery ◽  
Vesicle Dynamics ◽  
Image Correlation Spectroscopy ◽  
Spatiotemporal Image Correlation

Determination of Dynamics of Plant Plasma Membrane Proteins with Fluorescence Recovery and Raster Image Correlation Spectroscopy

2016 ◽  
Vol 22 (2) ◽  
pp. 290-299 ◽  
Author(s):  
Martina Laňková ◽  
Jana Humpolíčková ◽  
Stanislav Vosolsobě ◽  
Zdeněk Cit ◽  
Jozef Lacek ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Laser Scanning ◽  
Correlation Spectroscopy ◽  
Laser Scanning Microscopy ◽  
Image Correlation ◽  
Fluorescence Recovery ◽  
Confocal Laser ◽  
Raster Image ◽  
Image Correlation Spectroscopy ◽  
Plant Plasma Membrane

AbstractA number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more widely used fluorescence recovery after photobleaching method. For RICS, a set of microscopy images is obtained by single-photon confocal laser scanning microscopy (CLSM). Fluorescence fluctuations are subsequently correlated between individual pixels and the information on protein mobility are extracted using a model that considers processes generating the fluctuations such as diffusion and chemical binding reactions. As we show here using an example of two integral PM transporters of the plant hormone auxin, RICS uncovered their distinct short-distance lateral mobility within the PM that is dependent on cytoskeleton and sterol composition of the PM. RICS, which is routinely accessible on modern CLSM instruments, thus represents a valuable approach for studies of dynamics of PM proteins in plants.

Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

2006 ◽  
Vol 84 (6) ◽  
pp. 825-831 ◽  
Author(s):  
Ian R. Bates ◽  
Paul W. Wiseman ◽  
John W. Hanrahan
Keyword(s):  
Membrane Proteins ◽  
Fluorescence Correlation Spectroscopy ◽  
Fluorescence Recovery After Photobleaching ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Fluorescence Correlation ◽  
Transport Dynamics ◽  
Health And Disease ◽  
Image Correlation Spectroscopy ◽  
Selection Of

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

Easy Monitoring of Velocity Fields in Microfluidic Devices Using Spatiotemporal Image Correlation Spectroscopy

2013 ◽  
Vol 85 (17) ◽  
pp. 8080-8084 ◽  
Author(s):  
Marco Travagliati ◽  
Salvatore Girardo ◽  
Dario Pisignano ◽  
Fabio Beltram ◽  
Marco Cecchini
Keyword(s):  
Microfluidic Devices ◽  
Velocity Fields ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Image Correlation Spectroscopy ◽  
Spatiotemporal Image Correlation

Exploring oligomeric state of the serotonin1A receptor utilizing photobleaching image correlation spectroscopy: implications for receptor function

2018 ◽  
Vol 207 ◽  
pp. 409-421 ◽  
Author(s):  
Hirak Chakraborty ◽  
Md. Jafurulla ◽  
Andrew H. A. Clayton ◽  
Amitabha Chattopadhyay
Keyword(s):  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Membrane Cholesterol ◽  
Receptor Function ◽  
Oligomeric State ◽  
Serotonin1a Receptor ◽  
Image Correlation Spectroscopy

Photobleaching image correlation spectroscopy (pbICS) reveals that membrane cholesterol modulates the oligomeric state of the serotonin1A receptor.

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