Label-free in vivo imaging of Drosophila melanogaster by multiphoton microscopy

2008 ◽  
Author(s):  
Chiao-Ying Lin ◽  
Vladimir Hovhannisyan ◽  
June-Tai Wu ◽  
Sung-Jan Lin ◽  
Chii-Wann Lin ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
In Vivo Imaging ◽  
Multiphoton Microscopy ◽  
Label Free

In Vivo Imaging of Alzheimer Pathology in Transgenic Mice using Multiphoton Microscopy

2004 ◽  
pp. 33-45
Author(s):  
Brian J. Bacskai ◽  
William E. Klunk ◽  
Gregory A. Hickey ◽  
Jesse Skoch ◽  
Stephen T. Kajdasz ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
In Vivo Imaging ◽  
Multiphoton Microscopy ◽  
Alzheimer Pathology

MULTIPHOTON MICROSCOPY: A NEW APPROACH, IN PHYSIOLOGICAL STUDIES AND PATHOLOGICAL DIAGNOSIS FOR OPHTHALMOLOGY

2009 ◽  
Vol 02 (01) ◽  
pp. 45-60 ◽  
Author(s):  
CHIU-MEI HSUEH ◽  
WEN LO ◽  
SUNG-JAN LIN ◽  
TSUNG-JEN WANG ◽  
FUNG-RUNG HU ◽  
...  
Keyword(s):  
Second Harmonic ◽  
Healing Process ◽  
Multiphoton Microscopy ◽  
In Vivo Studies ◽  
Wound Healing Process ◽  
Label Free ◽  
Multiphoton Imaging ◽  
Morphological Alterations ◽  
Corneal Scar

Multiphoton microscopy (MPM), with the advantages of improved penetration depth, decreased photo-damage, and optical sectioning capability, has become an indispensable tool for biomedical imaging. The combination of multiphoton fluorescence (MF) and second-harmonic generation (SHG) microscopy is particularly effective in imaging tissue structures of the ocular surface. This work is intended to be a review of advances that MPM has made in ophthalmic imaging. The MPM not only can be used for the label-free imaging of ocular structures, it can also be applied for investigating the morphological alterations in corneal pathologies, such as keratoconus, infected keratitis, and corneal scar. Furthermore, the corneal wound healing process after refractive surgical procedures such as conductive keratoplasty (CK) can also be studied with MPM. Finally, qualitative and quantitative SHG microscopy is effective for characterizing corneal thermal denaturation. With additional development, multiphoton imaging has the potential to be developed into an effective imaging technique for in vivo studies and clinical diagnosis in ophthalmology.

scholarly journals In vivo imaging and tracking of individual nanodiamonds in drosophila melanogaster embryos

2014 ◽  
Vol 5 (4) ◽  
pp. 1250 ◽  
Author(s):  
David A. Simpson ◽  
Amelia J. Thompson ◽  
Mark Kowarsky ◽  
Nida F. Zeeshan ◽  
Michael S. J. Barson ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
In Vivo Imaging

In Vivo Imaging of Amyloid-β Deposits in Mouse Brain With Multiphoton Microscopy

2005 ◽  
pp. 349-364 ◽  
Author(s):  
Jesse Skoch ◽  
Gregory A. Hickey ◽  
Stephen T. Kajdasz ◽  
Bradley T. Hyman ◽  
Brian J. Bacskai
Keyword(s):  
Mouse Brain ◽  
In Vivo Imaging ◽  
Multiphoton Microscopy ◽  
Amyloid Β
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