A modified commercial ultrasound scanner used for in vivo photoacoustic imaging of nude mice injected with non-targeted contrast agents

2008 ◽  
Author(s):  
Ladislav Jankovic ◽  
Khalid Shahzad ◽  
Yao Wang ◽  
Michael Burcher ◽  
Frank-Detlef Scholle ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Nude Mice ◽  
Photoacoustic Imaging ◽  
Targeted Contrast Agents ◽  
Ultrasound Scanner

In vivo photoacoustic imaging of nude mice vasculature using a photoacoustic imaging system based on a commercial ultrasound scanner

2008 ◽  
Author(s):  
Ladislav Jankovic ◽  
Khalid Shahzad ◽  
Yao Wang ◽  
Michael Burcher ◽  
Frank-Detlef Scholle ◽  
...  
Keyword(s):  
Nude Mice ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Ultrasound Scanner

Styryl BODIPY Derivatives as Contrast Agents for In Vivo Photoacoustic Imaging

2017 ◽  
Author(s):  
Maryam Hatamimoslehabadi ◽  
Stephanie Bellinger ◽  
Jonathan Rochford ◽  
Chandra S Yelleswarapu
Keyword(s):  
Contrast Agents ◽  
Photoacoustic Imaging

scholarly journals Organic nanoparticle-doped microdroplets as dual-modality contrast agents for ultrasound microvascular flow and photoacoustic imaging

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu Xu ◽  
Guoyun Sun ◽  
Eshu Middha ◽  
Yu-Hang Liu ◽  
Kim Chuan Chan ◽  
...  
Keyword(s):  
Blood Flow ◽  
Nude Mice ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Contrast Effects ◽  
In Vivo Evaluation ◽  
Dual Modality ◽  
In Vivo Experiments

Abstract Tumor blood vessels are chaotic and abundantly distributed, owing to their heterogeneity. Therefore, imaging techniques which reveal abnormalities of tumor vasculature play significant roles in both mechanistic and clinical diagnostic tumor studies. Photoacoustic (PA) imaging uses the intrinsic characteristics of hemoglobin, to acquire tumor hemodynamic information, while ultrasound (US) imaging provides information about tumoral vessel structures and blood flow. To improve the imaging contrast performance, hydrogel-based microdroplets were designed for both US blood flow and PA imaging in this study. The microdroplets served as carriers for PA contrast agent solution in the innermost part while oil and hydrogel formed the inner and outer layers of the droplets. In vitro experiments firstly demonstrated the dual modality contrast effects of the microdroplets on US flow determination and PA imaging. In vivo experiments were then carried out in both healthy nude mice and nude mice with subcutaneous tumor to validate the contrast effects and to monitor the duration of contrast effects in animals. Using the dual-modality microdroplets, we were able to obtain distinct edges of tumor and blood flow mapping of the tumor microvascular with improved sensitivity up to 11.09 dB for PA and 6.69 dB for US flow. Besides, the in vivo evaluation with microdroplets showed US flow enhancement for more than 60 min. Therefore, the microdroplets are able to provide the contrast effects for both US flow and PA in a relative long duration and have potential to be applied in the tumor related diagnoses and studies.

scholarly journals Current concepts in nanostructured contrast media development for in vivo photoacoustic imaging

2019 ◽  
Vol 7 (5) ◽  
pp. 1746-1775 ◽  
Author(s):  
Mirko Maturi ◽  
Erica Locatelli ◽  
Ilaria Monaco ◽  
Mauro Comes Franchini
Keyword(s):  
Contrast Media ◽  
Contrast Agents ◽  
Photoacoustic Imaging ◽  
Media Development ◽  
Species Specific

To overcome the endogenous photoacoustic contrast arising from endogenous species, specific contrast agents need to be developed, allowing PAI to successfully identify targeted contrast in the range of wavelength in which the interference from the biomatrix is minimized.

scholarly journals Ultrasonic Imaging of Endothelial CD81 Expression Using CD81-Targeted Contrast Agents in In Vitro and In Vivo Studies

2012 ◽  
Vol 38 (4) ◽  
pp. 670-680 ◽  
Author(s):  
Fei Yan ◽  
Xiang Li ◽  
Qiaofeng Jin ◽  
Juanjuan Chen ◽  
Robin Shandas ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Ultrasonic Imaging ◽  
In Vivo Studies ◽  
Targeted Contrast Agents

Molybdenum Dioxide (MoS2)/Gadolinium (Gd) Containing Arginine-Glycine-Aspartic Acid (RGD) Sequences as New Nano-Contrast Agent for Cancer Magnetic Resonance Imaging (MRI)

2021 ◽  
Vol 21 (3) ◽  
pp. 1403-1412
Author(s):  
Xiaoguang Hao ◽  
Weijing Li
Keyword(s):  
Particle Size ◽  
Contrast Agent ◽  
Contrast Agents ◽  
Aspartic Acid ◽  
Nude Mice ◽  
Molybdenum Dioxide ◽  
Signal Value ◽  
Arginine Glycine Aspartic Acid

Molybdenum dioxide-gadolinium-arginine/glycine/aspartic acid (MoS2-Gd-RGD) sequences targeting nano-contrast agents that specifically bind to human hepatocellular carcinoma (HCC) HepG2 cells were synthesized, and their targeting imaging effects on HCC cells and models were evaluated. Zeta potential, particle size and Fourier Transform Infrared Spectrometer (FTIR) were used to characterize the nano-contrast agent, and its cytotoxicity was evaluated. The MoS2-Gd nanoparticles were used as control in vitro to determine the targeting capability of the MoS2-Gd-RGD nanoparticles toward integrin αvβ3. During in vivo animal experiments, 12 nude mice with tumors were randomly divided into three groups to compare the imaging effects of the MoS2-Gd-RGD and MoS2-Gd groups. The hydrodynamic diameter of MoS2-Gd-RGD nanoparticles was approximately 336.43±6.43 nm, and the polydispersity index (PDI) value reached 0.132. Transmission electron microscopy showed the uniform particle size and good dispersion of the nanoparticles. The relaxation rate totaled 1.39 mM−1S−1. The signal value of the T1-weighted image of the HepG2 cells treated with MoS2-Gd-RGD was higher than that of the non-targeted materials (MoS2-Gd) (P < 0.01). The signal value of the tumor increased significantly 15 min after the tail vein injection with MoS2-Gd-RGD, and it peaked at 60 min after injection. A significant difference in tumor signal values was observed between the two groups of nude mice injected with MoS2-Gd-RGD and MoS2- Gd (P < 0.01). At the in vitro and in vivo experiments, the MoS2-Gd-RGD nanoparticles presented the characteristics of integrin αvβ3 targeting. Thus, MoS2-Gd-RGD nanoparticles feature potential as contrast agents for MRI.

Push–pull type meso-ester substituted BODIPY near-infrared dyes as contrast agents for photoacoustic imaging

2017 ◽  
Vol 15 (21) ◽  
pp. 4531-4535 ◽  
Author(s):  
Yong Ni ◽  
Ravi Kumar Kannadorai ◽  
Sidney W.-K. Yu ◽  
Young-Tae Chang ◽  
Jishan Wu
Keyword(s):  
Contrast Agents ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Nir Absorption ◽  
Photo Stability

Push–pull meso-ester BODIPYs with intense NIR absorption and good photo-stability were used for in vitro and in vivo photoacoustic imaging.

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