Development of microfluidic structures for high throughput flow cytometric characterization of blood cells

2007 ◽  
Author(s):  
A. Kummrow ◽  
H. Yildirim ◽  
J. Theisen ◽  
K. Brattke ◽  
C. Sprenger ◽  
...  
Keyword(s):  
High Throughput ◽  
Blood Cells ◽  
Flow Cytometric

scholarly journals High-throughput linear optical stretcher for mechanical characterization of blood cells

2015 ◽  
Vol 89 (4) ◽  
pp. 391-397 ◽  
Author(s):  
Kevin B. Roth ◽  
Keith B. Neeves ◽  
Jeff Squier ◽  
David W. M. Marr
Keyword(s):  
High Throughput ◽  
Blood Cells ◽  
Mechanical Characterization ◽  
Optical Stretcher ◽  
Linear Optical

Development of microfluidic structures for high throughput flow cy-tometric characterization of blood cells

2007 ◽  
Author(s):  
A. Kummrow ◽  
H. Yildirim ◽  
J. Theisen ◽  
K. Brattke ◽  
C. Sprenger ◽  
...  
Keyword(s):  
High Throughput ◽  
Blood Cells

scholarly journals High-throughput microfluidic characterization of erythrocyte shape and mechanical variability

2018 ◽  
Author(s):  
Felix Reichel ◽  
Johannes Mauer ◽  
Ahmad Ahsan Nawaz ◽  
Gerhard Gompper ◽  
Jochen Guck ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Red Blood Cells ◽  
High Throughput ◽  
Blood Cells ◽  
Microvascular Blood Flow ◽  
Flow Conditions ◽  
Wide Range ◽  
Single Well ◽  
Good Agreement

The motion of red blood cells (RBCs) in microchannels is important for microvascular blood flow and biomedical applications such as blood analysis in microfluidics. The current understanding of the complexity of RBC shapes and dynamics in microchannels is mainly based on several simulation studies, but there are a few systematic experimental investigations. Here, we present a combined study, which systematically characterizes RBC behavior for a wide range of flow rates and channel sizes. Even though simulations and experiments generally show good agreement, experimental observations demonstrate that there is no single well-defined RBC state for fixed flow conditions, but rather a broad distribution of states. This result can be attributed to the inherent variability in RBC mechanical properties, which is confirmed by a model that takes the variation in RBC shear elasticity into account. This represents a significant step toward a quantitative connection between RBC behavior in microfluidic devices and their mechanical properties, which is essential for a high-throughput characterization of diseased cells.Significance StatementThe ability to change shape is crucial for the proper functioning of red blood cells under harsh conditions in the microvasculature, since their shapes strongly affect the flow behavior of whole blood. Our results from simulations and systematic experiments reveal the shapes and dynamics of red blood cells for different flow conditions and channel dimensions, generally in good agreement. However, in the experiments, cells do not exhibit a single well-defined shape for fixed flow conditions. We show that this distribution of shapes can be attributed to the variability in mechanical properties of red blood cells.

Combinatorial Fabrication and High-Throughput Characterization of Thin Film Metal Oxide Libraries for Solar water Splitting

2018 ◽  
Author(s):  
Alfred Ludwig ◽  
Mona Nowak ◽  
Swati Kumari ◽  
Helge S. Stein ◽  
Ramona Gutkowski ◽  
...  
Keyword(s):  
Thin Film ◽  
Metal Oxide ◽  
Water Splitting ◽  
High Throughput ◽  
Solar Water ◽  
Solar Water Splitting

scholarly journals Database of ab initio L-edge X-ray absorption near edge structure

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yiming Chen ◽  
Chi Chen ◽  
Chen Zheng ◽  
Shyam Dwaraknath ◽  
Matthew K. Horton ◽  
...  
Keyword(s):  
High Throughput ◽  
Scattering Theory ◽  
Research Community ◽  
Metal Compounds ◽  
Edge Structure ◽  
X Ray ◽  
Initial Release ◽  
Computational Workflow ◽  
X Ray Absorption

AbstractThe L-edge X-ray Absorption Near Edge Structure (XANES) is widely used in the characterization of transition metal compounds. Here, we report the development of a database of computed L-edge XANES using the multiple scattering theory-based FEFF9 code. The initial release of the database contains more than 140,000 L-edge spectra for more than 22,000 structures generated using a high-throughput computational workflow. The data is disseminated through the Materials Project and addresses a critical need for L-edge XANES spectra among the research community.

High-Throughput Synthesis and Characterization of a Combinatorial Materials Library in Bulk Alloys

2021 ◽  
Vol 52 (4) ◽  
pp. 1159-1168
Author(s):  
Lei Zhao ◽  
Yuanxun Zhou ◽  
Hui Wang ◽  
Xuebin Chen ◽  
Lixia Yang ◽  
...  
Keyword(s):  
High Throughput ◽  
Synthesis And Characterization ◽  
Combinatorial Materials ◽  
Bulk Alloys

scholarly journals Molecular characterization of Polychromophilus parasites of Scotophilus kuhlii bats in Thailand

Parasitology ◽  
2020 ◽  
pp. 1-5
Author(s):  
Chatree Chumnandee ◽  
Nawarat Pha-obnga ◽  
Oskar Werb ◽  
Kai Matuschewski ◽  
Juliane Schaer
Keyword(s):  
Blood Cells ◽  
Clinical Symptoms ◽  
Temperate Climate ◽  
Diverse Group ◽  
Malaria Parasites ◽  
Climate Zones ◽  
Haemosporidian Parasites ◽  
South And Southeast Asia ◽  
Molecular Investigation

Abstract Parasites of the haemosporidian genus Polychromophilus have exclusively been described in bats. These parasites belong to the diverse group of malaria parasites, and Polychromophilus presents the only haemosporidian taxon that infects mammalian hosts in tropical as well as in temperate climate zones. This study provides the first information of Polychromophilus parasites in the lesser Asiatic yellow bat (Scotophilus kuhlii) in Thailand, a common vespertilionid bat species distributed in South and Southeast Asia. The gametocyte blood stages of the parasites could not be assigned to a described morphospecies and molecular analysis revealed that these parasites might represent a distinct Polychromophilus species. In contrast to Plasmodium species, Polychromophilus parasites do not multiply in red blood cells and, thus, do not cause the clinical symptoms of malaria. Parasitological and molecular investigation of haemosporidian parasites of wildlife, such as the neglected genus Polychromophilus, will contribute to a better understanding of the evolution of malaria parasites.

Sonoporation enables high-throughput loading of trehalose into red blood cells

Cryobiology ◽  
2020 ◽  
Author(s):  
Brett R. Janis ◽  
Mariah C. Priddy ◽  
Meghan R. Otto ◽  
Jonathan A. Kopechek ◽  
Michael A. Menze
Keyword(s):  
Red Blood Cells ◽  
High Throughput ◽  
Blood Cells
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