scholarly journals Utilizing nonlinear optical microscopy to investigate the development of early cancer in nude mice in vivo

2007 ◽  
Author(s):  
Chun-Chin Wang ◽  
Feng-Chieh Li ◽  
Sung-Jan Lin ◽  
Wen Lo ◽  
Chen-Yuan Dong
Keyword(s):  
Optical Microscopy ◽  
Nude Mice ◽  
Nonlinear Optical ◽  
Early Cancer ◽  
Nonlinear Optical Microscopy

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

In-vivo monitoring rat skin wound healing using nonlinear optical microscopy

2014 ◽  
Author(s):  
Jing Chen ◽  
Chungen Guo ◽  
Fan Zhang ◽  
Yahao Xu ◽  
Xiaoqin Zhu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Skin Wound ◽  
Rat Skin ◽  
Skin Wound Healing ◽  
Nonlinear Optical Microscopy ◽  
In Vivo Monitoring

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

scholarly journals Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1105
Author(s):  
Pálma Anker ◽  
Luca Fésűs ◽  
Norbert Kiss ◽  
Judit Noll ◽  
Krisztina Becker ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Diffuse Reflectance ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Light Emitting Diode ◽  
Autofluorescence Imaging ◽  
Nonlinear Optical Microscopy ◽  
Keratinopathic Ichthyosis

Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical microscopy. Two-photon excited fluorescence and second-harmonic generation can visualize epidermal keratin and dermal collagen, respectively. We were able to visualize the structure of the epidermis and other skin changes caused by abnormal keratin formation. Taken together, we were able to show that such imaging modalities are useful for the diagnosis and follow-up of keratinopathic diseases.

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