In vivo high-resolution multimodal nonlinear optical microscopy of spinal cord in mice

2020 ◽  
Author(s):  
Wanjie Wu ◽  
Zhongya Qin ◽  
Junqiang Wu ◽  
Congping Chen ◽  
Weitao Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
High Resolution ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

Spatial Overlap Modulation Nonlinear Optical Microscopy for High-resolution Deep Imaging

2013 ◽  
Author(s):  
Keisuke Isobe ◽  
Hiroyuki Kawano ◽  
Akira Suda ◽  
Akiko Kumagai ◽  
Hideaki Mizuno ◽  
...  
Keyword(s):  
High Resolution ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy ◽  
Spatial Overlap ◽  
Deep Imaging

scholarly journals Nonlinear Optical Microscopy Captures High-Resolution Images of Microstructures Within Three Types of Unlabeled Rat Cartilage

2016 ◽  
Vol 8 (3) ◽  
pp. 1-10 ◽  
Author(s):  
Xiaoqin Zhu ◽  
Liqin Zheng ◽  
Xinpeng Huang ◽  
Jianfeng Wu ◽  
Lihong Hong ◽  
...  
Keyword(s):  
High Resolution ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy ◽  
High Resolution Images

In-vivo monitoring rat skin wound healing using nonlinear optical microscopy

2014 ◽  
Author(s):  
Jing Chen ◽  
Chungen Guo ◽  
Fan Zhang ◽  
Yahao Xu ◽  
Xiaoqin Zhu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Microscopy ◽  
Nonlinear Optical ◽  
Skin Wound ◽  
Rat Skin ◽  
Skin Wound Healing ◽  
Nonlinear Optical Microscopy ◽  
In Vivo Monitoring
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