Compensation of global movement for improved tracking of cells in time-lapse confocal microscopy image sequences

2007 ◽  
Author(s):  
Il-Han Kim ◽  
William J. Godinez ◽  
Nathalie Harder ◽  
Felipe Mora-Bermúdez ◽  
Jan Ellenberg ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Time Lapse ◽  
Image Sequences ◽  
Microscopy Image ◽  
Global Movement ◽  
Confocal Microscopy Image

scholarly journals Lipid-based nanovesicles for nanomedicine

2016 ◽  
Vol 45 (23) ◽  
pp. 6520-6545 ◽  
Author(s):  
N. Grimaldi ◽  
F. Andrade ◽  
N. Segovia ◽  
L. Ferrer-Tasies ◽  
S. Sala ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Confocal Microscopy ◽  
Self Assembly ◽  
Sirna Transfection ◽  
Microscopy Image ◽  
Confocal Microscopy Image ◽  
Conventional Methods ◽  
Membrane Components

Multifunctional lipid-based nanovesicles (L-NVs) prepared by molecular self-assembly of membrane components together with (bio)-active molecules, by means of compressed CO2-media or other non-conventional methods lead to highly homogeneous, tailor-made nanovesicles that are used for advanced nanomedicine. Confocal microscopy image of siRNA transfection using L-NVs, reprinted with permission from de Jonge,et al.,Gene Therapy, 2006,13, 400–411.

scholarly journals Analysis of confocal microscopy image data of Physcomitrella chloroplasts to reveal adaptation principles leading to structural stability at the nanoscale

PAMM ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 69-70 ◽  
Author(s):  
Pouyan Asgharzadeh ◽  
Bugra Özdemir ◽  
Stefanie J. Müller ◽  
Ralf Reski ◽  
Oliver Röhrle
Keyword(s):  
Confocal Microscopy ◽  
Structural Stability ◽  
Image Data ◽  
Microscopy Image ◽  
Confocal Microscopy Image

Confocal microscopy, image restoration, and nuclear structure

1993 ◽  
Vol 51 ◽  
pp. 146-147
Author(s):  
Peter Shaw ◽  
Alison Beven ◽  
David Rawlins ◽  
Martin Highett
Keyword(s):  
Confocal Microscopy ◽  
Functional Organization ◽  
Confocal Imaging ◽  
Rrna Genes ◽  
Wide Field ◽  
Image Deconvolution ◽  
Microscopy Image ◽  
Confocal Images ◽  
Confocal Microscopy Image

Fluorescent in situ hybridization and 3-D confocal microscopy have been used to study the arrangement of genes and transcripts in interphase plant nuclei. Wide-field fluorescence microscopy coupled with computer deconvolution has also been used on the same specimens and gives comparable results to confocal imaging. When image deconvolution is applied to confocal images, these show a significant improvement. A comparison of confocal and wide-field (CCD) images of the same specimen (a plant nucleolus labelled with probe to the rRNA genes) is shown in Figure 1. The deconvoluted wide-field data (Fig 1b) and the raw confocal data (Fig 1c) are remarkably similar; the former is somewhat clearer. The deconvoluted confocal data (Fig 1d) is clearest of all.Double-stranded DNA and single-stranded RNA probes to the 45S and 5S RNA genes have been used to investigate the functional organization of the nucleolus in pea root tissue. In situ hybridization was carried out on vibratome slices approximately 50μm thick (about 3 cells).

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