scholarly journals In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy

2007 ◽  
Author(s):  
Wen Lo ◽  
Yuh-Ling Chang ◽  
Yen Sun ◽  
Sung-Jan Lin ◽  
Shiou-Hwa Jee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Corneal Wound Healing ◽  
Second Harmonic Generation Microscopy ◽  
Culture Model ◽  
Corneal Wound

Albumin precursor and Hsp70 modulate corneal wound healing in an organ culture model

2011 ◽  
Vol 113 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Shamim Mushtaq ◽  
Zulfiqar Ali Naqvi ◽  
Anwar Ali Siddiqui ◽  
Nikhat Ahmed
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Corneal Wound Healing ◽  
Culture Model ◽  
Corneal Wound

Epithelial-derived TGF-β2 modulates basal and wound-healing subepithelial matrix homeostasis

2006 ◽  
Vol 291 (6) ◽  
pp. L1277-L1285 ◽  
Author(s):  
H. Garrett R. Thompson ◽  
Justin D. Mih ◽  
Tatiana B. Krasieva ◽  
Bruce J. Tromberg ◽  
Steven C. George
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Transforming Growth Factor ◽  
Second Harmonic ◽  
Smooth Muscle Actin ◽  
Critical Role ◽  
The Matrix

The epithelium influences the mesenchyme during dynamic processes such as embryogenesis, wound healing, fibrosis, and carcinogenesis. Since transforming growth factor-β (TGF-β) modulates these processes, we hypothesized that epithelial-derived TGF-β also plays a critical role in maintaining the extracellular matrix at basal conditions. We utilized an in vitro model of the epithelial-mesenchymal trophic unit in the human airways to determine the role of epithelial-derived TGF-β in modulating the extracellular matrix under basal and wound-healing conditions. When differentiated at an air-liquid interface, the human bronchial epithelium produces active TGF-β2 at a concentration of 50–70 pg/ml, whereas TGF-β1 is undetectable. TGF-β2 increases two- to threefold following scrape injury in a dose-dependent fashion and significantly enhances both α-smooth muscle actin expression in the underlying collagen-embedded fibroblasts and secretion of tenascin-C into the matrix. Multiphoton microscopy demonstrates substantially enhanced second harmonic generation from fibrillar collagen in the matrix. Pretreatment of the matrix with either sirolimus (2.5 nM) or paclitaxel (10 nM) abolishes the increases in both TGF-β2 and second harmonic generation in response to epithelial injury. In the absence of the epithelium, exogenous active TGF-β2 (0–400 pg/ml) produces a biphasic response in the second harmonic signal with a minimum occurring at the epithelial-derived basal level. We conclude that epithelial-derived TGF-β2 is secreted in response to injury, significantly alters the bulk optical properties of the extracellular matrix, and its tight regulation may be required for normal collagen homeostasis.

Development of a Three-Dimensional Organ Culture Model for Corneal Wound Healing and Corneal Transplantation

2006 ◽  
Vol 47 (7) ◽  
pp. 2840 ◽  
Author(s):  
Bojun Zhao ◽  
Leanne J. Cooper ◽  
Arun Brahma ◽  
Sheila MacNeil ◽  
Stephen Rimmer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Corneal Transplantation ◽  
Three Dimensional ◽  
Corneal Wound Healing ◽  
Culture Model ◽  
Corneal Wound

Corneal wound healing is modulated by topical application of amniotic fluid in an ex vivo organ culture model

2008 ◽  
Vol 87 (1) ◽  
pp. 56-63 ◽  
Author(s):  
Juan Castro-Combs ◽  
Guillermo Noguera ◽  
Marisol Cano ◽  
Margaret Yew ◽  
Peter L. Gehlbach ◽  
...  
Keyword(s):  
Wound Healing ◽  
Organ Culture ◽  
Amniotic Fluid ◽  
Topical Application ◽  
Ex Vivo ◽  
Corneal Wound Healing ◽  
Culture Model ◽  
Corneal Wound
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