In vitro characterization of corneal wound healing using multiphoton autofluorescence and second harmonic generation (SHG) microscopy

2006 ◽  
Author(s):  
Yen Sun ◽  
Wen Lo ◽  
Wei-Liang Chen ◽  
Shu-Wen Teng ◽  
Hsin-Yuan Tan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Corneal Wound Healing ◽  
In Vitro Characterization ◽  
Corneal Wound

Epithelial-derived TGF-β2 modulates basal and wound-healing subepithelial matrix homeostasis

2006 ◽  
Vol 291 (6) ◽  
pp. L1277-L1285 ◽  
Author(s):  
H. Garrett R. Thompson ◽  
Justin D. Mih ◽  
Tatiana B. Krasieva ◽  
Bruce J. Tromberg ◽  
Steven C. George
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Transforming Growth Factor ◽  
Second Harmonic ◽  
Smooth Muscle Actin ◽  
Critical Role ◽  
The Matrix

The epithelium influences the mesenchyme during dynamic processes such as embryogenesis, wound healing, fibrosis, and carcinogenesis. Since transforming growth factor-β (TGF-β) modulates these processes, we hypothesized that epithelial-derived TGF-β also plays a critical role in maintaining the extracellular matrix at basal conditions. We utilized an in vitro model of the epithelial-mesenchymal trophic unit in the human airways to determine the role of epithelial-derived TGF-β in modulating the extracellular matrix under basal and wound-healing conditions. When differentiated at an air-liquid interface, the human bronchial epithelium produces active TGF-β2 at a concentration of 50–70 pg/ml, whereas TGF-β1 is undetectable. TGF-β2 increases two- to threefold following scrape injury in a dose-dependent fashion and significantly enhances both α-smooth muscle actin expression in the underlying collagen-embedded fibroblasts and secretion of tenascin-C into the matrix. Multiphoton microscopy demonstrates substantially enhanced second harmonic generation from fibrillar collagen in the matrix. Pretreatment of the matrix with either sirolimus (2.5 nM) or paclitaxel (10 nM) abolishes the increases in both TGF-β2 and second harmonic generation in response to epithelial injury. In the absence of the epithelium, exogenous active TGF-β2 (0–400 pg/ml) produces a biphasic response in the second harmonic signal with a minimum occurring at the epithelial-derived basal level. We conclude that epithelial-derived TGF-β2 is secreted in response to injury, significantly alters the bulk optical properties of the extracellular matrix, and its tight regulation may be required for normal collagen homeostasis.

Corneal Wound Healing in Vitro

1963 ◽  
Vol 56 (5) ◽  
pp. 833-834
Author(s):  
N.A. Wine ◽  
P.K. Basu
Keyword(s):  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound

IL-6 as a corneal wound healing mediator in an in vitro scratch assay

2014 ◽  
Vol 125 ◽  
pp. 183-192 ◽  
Author(s):  
Isabel Arranz-Valsero ◽  
Laura Soriano-Romaní ◽  
Laura García-Posadas ◽  
Antonio López-García ◽  
Yolanda Diebold
Keyword(s):  
Wound Healing ◽  
Scratch Assay ◽  
Corneal Wound Healing ◽  
Corneal Wound

scholarly journals Characterization of wavefront errors in mouse cranial bone using second-harmonic generation

2017 ◽  
Vol 22 (3) ◽  
pp. 036012 ◽  
Author(s):  
Kayvan Forouhesh Tehrani ◽  
Peter Kner ◽  
Luke J. Mortensen
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Cranial Bone
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