In vivo micro-CT imaging of the murine lung via a computer controlled intermittent iso-pressure breath hold (IIBH) technique

2006 ◽  
Author(s):  
Eman Namati ◽  
Deokiee Chon ◽  
Jacqueline Thiesse ◽  
Geoffrey McLennan ◽  
Jered Sieren ◽  
...  
Keyword(s):  
Ct Imaging ◽  
Breath Hold ◽  
Micro Ct ◽  
Murine Lung ◽  
Computer Controlled

scholarly journals Lung structure phenotype variation in inbred mouse strains revealed through in vivo micro-CT imaging

2010 ◽  
Vol 109 (6) ◽  
pp. 1960-1968 ◽  
Author(s):  
Jacqueline Thiesse ◽  
Eman Namati ◽  
Jessica C. Sieren ◽  
Amanda R. Smith ◽  
Joseph M. Reinhardt ◽  
...  
Keyword(s):  
Lung Function ◽  
Inbred Mouse ◽  
Small Animal Imaging ◽  
Ct Imaging ◽  
Mouse Strains ◽  
Breath Hold ◽  
Inbred Mouse Strains ◽  
Micro Ct ◽  
Lung Structure

Within pulmonary research, the development of mouse models has provided insight into disease development, progression, and treatment. Structural phenotypes of the lung in healthy inbred mouse strains are necessary for comparison to disease models. To date, progress in the assessment of lung function in these small animals using whole lung function tests has been made. However, assessment of in vivo lung structure of inbred mouse strains has yet to be well defined. Therefore, the link between the structure and function phenotypes is still unclear. With advancements in small animal imaging it is now possible to investigate lung structures such as the central and peripheral airways, whole lung, and lobar volumes of mice in vivo, through the use of micro-CT imaging. In this study, we performed in vivo micro-CT imaging of the C57BL/6, A/J, and BALB/c mouse strains using the intermittent iso-pressure breath hold (IIBH) technique. The resulting high-resolution images were used to extract lung structure phenotypes. The three-dimensional lobar structures and airways were defined and a meaningful mouse airway nomenclature was developed. In addition, using these techniques we have uncovered significant differences in the airway structures between inbred mouse strains in vivo.

scholarly journals Effects of long-term in vivo micro-CT imaging on hallmarks of osteopenia and frailty in aging mice

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0239534
Author(s):  
Ariane C. Scheuren ◽  
Gisela A. Kuhn ◽  
Ralph Müller
Keyword(s):  
Ct Imaging ◽  
Micro Ct

Radiosensitization and micro CT imaging of multifunctional gold nanoparticles in lung adenocarcinoma A549 cell: An in vivo animal study.

2018 ◽  
Vol 36 (15_suppl) ◽  
pp. e20557-e20557
Author(s):  
Tao Li ◽  
Chuan Yang ◽  
Guojun Zhang ◽  
Jianming Huang ◽  
Jiahua Lv ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lung Adenocarcinoma ◽  
Animal Study ◽  
A549 Cell ◽  
Ct Imaging ◽  
Micro Ct ◽  
Lung Adenocarcinoma A549

scholarly journals Longitudinal assessment of lung cancer progression in the mouse using in vivo micro-CT imaging

2010 ◽  
Vol 37 (9) ◽  
pp. 4793-4805 ◽  
Author(s):  
Eman Namati ◽  
Jacqueline Thiesse ◽  
Jessica C. Sieren ◽  
Alan Ross ◽  
Eric A. Hoffman ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Progression ◽  
Ct Imaging ◽  
Micro Ct ◽  
Longitudinal Assessment

scholarly journals Effects of long-term in vivo micro-CT imaging on hallmarks of osteopenia and frailty in aging mice

2020 ◽  
Author(s):  
Ariane C. Scheuren ◽  
Gisela A. Kuhn ◽  
Ralph Müller
Keyword(s):  
Body Weight ◽  
Aging Process ◽  
Frailty Index ◽  
Ct Imaging ◽  
Morphometric Parameters ◽  
The Body ◽  
The Other ◽  
Micro Ct

AbstractIn vivo micro-CT has already been used to monitor microstructural changes of bone in mice of different ages and in models of age-related diseases such as osteoporosis. However, as aging is accompanied by frailty and subsequent increased sensitivity to external stimuli such as handling and anesthesia, the extent to which longitudinal imaging can be applied in aging studies remains unclear. Consequently, the potential of monitoring individual mice during the entire aging process – from healthy to frail status – has not yet been exploited. In this study, we assessed the effects of long-term in vivo micro-CT imaging - consisting of 11 imaging sessions over 20 weeks - on hallmarks of aging both on a local (i.e., static and dynamic bone morphometry) and systemic (i.e., frailty index (FI) and body weight) level at various stages of the aging process. Furthermore, using a premature aging model (PolgA(D257A/D257A)), we assessed whether these effects differ between genotypes.The 6th caudal vertebrae of 4 groups of mice (PolgA(D257A/D257A) and PolgA(+/+)) were monitored by in vivo micro-CT every 2 weeks. One group was subjected to 11 scans between weeks 20 and 40 of age, whereas the other groups were subjected to 5 scans between weeks 26-34, 32-40 and 40-46, respectively. The long-term monitoring approach showed small but significant changes in the static bone morphometric parameters compared to the other groups. However, no interaction effect between groups and genotype was found, suggesting that PolgA mutation does not render bone more or less susceptible to long-term micro-CT imaging. The differences between groups observed in the static morphometric parameters were less pronounced in the dynamic morphometric parameters. Moreover, the body weight and FI were not affected by more frequent imaging sessions. Finally, we observed that longitudinal designs including baseline measurements at young adult age are more powerful at detecting effects of in vivo micro-CT imaging on hallmarks of aging than cross-sectional comparisons between multiple groups of aged mice subjected to fewer imaging sessions.

scholarly journals Intratumoral diffusion patterns of small-molecule surface passivated Gold Nanoparticles quantified using in vivo micro-CT imaging

2021 ◽  
Author(s):  
Rossana Terracciano ◽  
Aobo Zang ◽  
E. Brian Butler ◽  
Danilo Demarchi ◽  
Jason Hafner ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Ct Imaging ◽  
Micro Ct ◽  
Molecule Surface

In vivo micro-CT imaging of rat brain glioma: A comparison with 3T MRI and histology

2009 ◽  
Vol 458 (1) ◽  
pp. 28-31 ◽  
Author(s):  
Tobias Engelhorn ◽  
Ilker Y. Eyupoglu ◽  
Marc A. Schwarz ◽  
Marek Karolczak ◽  
Holger Bruenner ◽  
...  
Keyword(s):  
Rat Brain ◽  
Ct Imaging ◽  
Micro Ct ◽  
Brain Glioma ◽  
3T Mri

scholarly journals In Vivo Micro-CT Imaging of Human Mesenchymal Stem Cells Labeled with Gold-Poly-l-Lysine Nanocomplexes

2016 ◽  
Vol 27 (3) ◽  
pp. 1604213 ◽  
Author(s):  
Taeho Kim ◽  
Nohyun Lee ◽  
Dian R. Arifin ◽  
Irina Shats ◽  
Miroslaw Janowski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Ct Imaging ◽  
Micro Ct
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