Surface enhanced Raman spectroscopy (SERS) for the detection of intracellular constituents using gold nanoshells

2006 ◽  
Author(s):  
Mustafa H. Chowdhury ◽  
Colin J. Campbell ◽  
Eirini Theofanidou ◽  
Seung Joon Lee ◽  
Angela Baldwin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanoshells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Surface Enhanced Raman Spectroscopy and Intracellular Components

Proceedings ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 14
Author(s):  
Mario D’Acunto
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biological Agents ◽  
Small Sample ◽  
Gold Nanoshells ◽  
Label Free ◽  
Sample Sizes ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Small Sample Sizes

In the last decade, surface-enhanced Raman spectroscopy (SERS) met increasing interest in the detection of chemical and biological agents due to its rapid performance and ultra-sensitive features. SERS is a combination of Raman spectroscopy and nanotechnology; it includes the advantages of Raman spectroscopy, providing rapid spectra collection, small sample sizes, and characteristic spectral fingerprints for specific analytes. In this paper, we detected label-free SERS signals for arbitrarily configurations of dimers, trimers, etc., composed of gold nanoshells (AuNSs) and applied to the mapping of osteosarcoma intracellular components.

Using Catalytic and Surface-Enhanced Raman Spectroscopy-Active Gold Nanoshells to Understand the Role of Basicity in Glycerol Oxidation

ACS Catalysis ◽  
2013 ◽  
Vol 3 (11) ◽  
pp. 2430-2435 ◽  
Author(s):  
Kimberly N. Heck ◽  
Benjamin G. Janesko ◽  
Gustavo E. Scuseria ◽  
Naomi J. Halas ◽  
Michael S. Wong
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanoshells ◽  
Glycerol Oxidation ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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