A 16-channel avalanche photodiode detector array for visible and near-infrared flow cytometry

2006 ◽  
Author(s):  
William G. Lawrence ◽  
Christopher Stapels ◽  
Richard Farrell ◽  
Joseph D. Tario, Jr. ◽  
Edward Podniesinski ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Avalanche Photodiode ◽  
Detector Array

Monolithically integrated near-infrared and mid-infrared detector array for spectral imaging

2007 ◽  
Vol 50 (2-3) ◽  
pp. 211-216 ◽  
Author(s):  
S.V. Bandara ◽  
S.D. Gunapala ◽  
D.Z. Ting ◽  
J.K. Liu ◽  
C.J. Hill ◽  
...  
Keyword(s):  
Near Infrared ◽  
Infrared Detector ◽  
Spectral Imaging ◽  
Detector Array ◽  
Mid Infrared ◽  
Monolithically Integrated ◽  
Infrared Detector Array

scholarly journals Enhanced red and near infrared detection in flow cytometry using avalanche photodiodes

2008 ◽  
Vol 73A (8) ◽  
pp. 767-776 ◽  
Author(s):  
William G. Lawrence ◽  
Gyula Varadi ◽  
Gerald Entine ◽  
Edward Podniesinski ◽  
Paul K. Wallace
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Avalanche Photodiodes ◽  
Infrared Detection

Monolithically integrated near-infrared and mid-infrared detector array

2005 ◽  
Author(s):  
S. V. Bandara ◽  
S. D. Gunapala ◽  
D Z. Ting ◽  
J. K. Liu ◽  
C. J. Hill ◽  
...  
Keyword(s):  
Near Infrared ◽  
Infrared Detector ◽  
Detector Array ◽  
Mid Infrared ◽  
Monolithically Integrated ◽  
Infrared Detector Array

scholarly journals Imaging Cardiovascular and Lung Macrophages With the Positron Emission Tomography Sensor 64 Cu-Macrin in Mice, Rabbits, and Pigs

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Matthias Nahrendorf ◽  
Friedrich Felix Hoyer ◽  
Anu E. Meerwaldt ◽  
Mandy M.T. van Leent ◽  
Max L. Senders ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Pet Imaging ◽  
Near Infrared ◽  
Emission Tomography ◽  
Imaging Biomarker ◽  
Positron Emission ◽  
Pulmonary Macrophages

Background: Macrophages, innate immune cells that reside in all organs, defend the host against infection and injury. In the heart and vasculature, inflammatory macrophages also enhance tissue damage and propel cardiovascular diseases. Methods: We here use in vivo positron emission tomography (PET) imaging, flow cytometry, and confocal microscopy to evaluate quantitative noninvasive assessment of cardiac, arterial, and pulmonary macrophages using the nanotracer 64 Cu-Macrin—a 20-nm spherical dextran nanoparticle assembled from nontoxic polyglucose. Results: PET imaging using 64 Cu-Macrin faithfully reported accumulation of macrophages in the heart and lung of mice with myocardial infarction, sepsis, or pneumonia. Flow cytometry and confocal microscopy detected the near-infrared fluorescent version of the nanoparticle ( VT680 Macrin) primarily in tissue macrophages. In 5-day-old mice, 64 Cu-Macrin PET imaging quantified physiologically more numerous cardiac macrophages. Upon intravenous administration of 64 Cu-Macrin in rabbits and pigs, we detected heightened macrophage numbers in the infarcted myocardium, inflamed lung regions, and atherosclerotic plaques using a clinical PET/magnetic resonance imaging scanner. Toxicity studies in rats and human dosimetry estimates suggest that 64 Cu-Macrin is safe for use in humans. Conclusions: Taken together, these results indicate 64 Cu-Macrin could serve as a facile PET nanotracer to survey spatiotemporal macrophage dynamics during various physiological and pathological conditions. 64 Cu-Macrin PET imaging could stage inflammatory cardiovascular disease activity, assist disease management, and serve as an imaging biomarker for emerging macrophage-targeted therapeutics.

scholarly journals Infrared detector activities at NASA Langley Research Center

2008 ◽  
Vol 1076 ◽  
Author(s):  
M. Nurul Abedin ◽  
Tamer F Refaat ◽  
Oleg V Sulima ◽  
Farzin Amzajerdian
Keyword(s):  
Remote Sensing ◽  
Near Infrared ◽  
Infrared Detector ◽  
Avalanche Photodiode ◽  
Low Noise ◽  
Focal Plane Arrays ◽  
Research Center ◽  
Single Element ◽  
Sensing Applications ◽  
Langley Research Center

ABSTRACTInfrared detector development and characterization at NASA Langley Research Center will be reviewed. These detectors were intended for ground, airborne, and space borne remote sensing applications. Discussion will be focused on recently developed single-element infrared detector and future development of near-infrared focal plane arrays (FPA). The FPA will be applied to next generation space-based instruments. These activities are based on phototransistor and avalanche photodiode technologies, which offer high internal gain and relatively low noise-equivalent-power. These novel devices will improve the sensitivity of active remote sensing instruments while eliminating the need for a high power laser transmitter.

Enhancing near-infrared avalanche photodiode performance by femtosecond laser microstructuring

2006 ◽  
Vol 45 (35) ◽  
pp. 8825 ◽  
Author(s):  
Richard A. Myers ◽  
Richard Farrell ◽  
Arieh M. Karger ◽  
James E. Carey ◽  
Eric Mazur
Keyword(s):  
Femtosecond Laser ◽  
Near Infrared ◽  
Avalanche Photodiode

scholarly journals Multiparametric Flow Cytometry Using Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122342 ◽  
Author(s):  
William G. Telford ◽  
Daria M. Shcherbakova ◽  
David Buschke ◽  
Teresa S. Hawley ◽  
Vladislav V. Verkhusha
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Multiparametric Flow Cytometry ◽  
Bacterial Phytochromes

scholarly journals In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Manuelle Debunne ◽  
Christophe Portal ◽  
Bruno Delest ◽  
Ebba Brakenhielm ◽  
Françoise Lallemand ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Endothelial Cells ◽  
Caspase 3 ◽  
Near Infrared ◽  
Ex Vivo ◽  
Annexin V ◽  
Fluorescent Signal ◽  
Infra Red ◽  
Infrared Probes

Purpose. The aim of this paper is to develop new optical bioprobes for the imaging of apoptosis. Procedure. We developed quenched near-infrared probes which become fluorescent upon cleavage by caspase-3, the key regulatory enzyme of apoptosis. Results. Probes were shown to be selectively cleaved by recombinant caspase-3. Apoptosis of cultured endothelial cells was associated with an increased fluorescent signal for the cleaved probes, which colocalized with caspase-3 and was reduced by the addition of a caspase-3 inhibitor. Flow cytometry demonstrated a similar profile between the cleaved probes and annexin V. Ex vivo experiments showed that sections of hearts obtained from mice treated with the proapoptotic drug doxorubicin displayed an increase in the fluorescent signal for the cleaved probes, which was reduced by a caspase-3 inhibitor. Conclusion. We demonstrated the capacity of these novel probes to detect apoptosis by optical imaging in vitro and ex vivo.

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