Anterior segment imaging in vivo in rats and ex vivo in mice using full-field optical coherence tomography

2006 ◽  
Author(s):  
Kate Grieve ◽  
Petr Kuchynka ◽  
Manuel Simonutti ◽  
Arnaud Dubois ◽  
Jean-François Le Gargasson ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Anterior Segment ◽  
Optical Coherence ◽  
Full Field ◽  
Anterior Segment Imaging

scholarly journals In vivo anterior segment imaging in the rat eye with high speed white light full-field optical coherence tomography

2005 ◽  
Vol 13 (16) ◽  
pp. 6286 ◽  
Author(s):  
Kate Grieve ◽  
Arnaud Dubois ◽  
Manuel Simonutti ◽  
Michel Paques ◽  
José Sahel ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
White Light ◽  
High Speed ◽  
Anterior Segment ◽  
Optical Coherence ◽  
Full Field ◽  
Anterior Segment Imaging

scholarly journals Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents

2020 ◽  
Vol 245 (18) ◽  
pp. 1629-1636
Author(s):  
Ruiming Kong ◽  
Wenjuan Wu ◽  
Rui Qiu ◽  
Lei Gao ◽  
Fengxian Du ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Light Attenuation ◽  
Anterior Segment ◽  
Posterior Segment ◽  
Glycerol Solution ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Imaging Depth

Optical coherence tomography has become an indispensable diagnostic tool in ophthalmology for imaging the retina and the anterior segment of the eye. However, the imaging depth of optical coherence tomography is limited by light attenuation in tissues due to optical scattering and absorption. In this study of rabbit eye both ex vivo and in vivo, optical coherence tomography imaging depth of the anterior and posterior segments of the eye was extended by using optical clearing agents to reduce multiple scattering. The sclera, the iris, and the ciliary body were clearly visualized by direct application of glycerol at an incision on the conjunctiva, and the posterior boundary of sclera and even the deeper tissues were detected by submerging the posterior segment of eye in glycerol solution ex vivo or by retro-bulbar injection of glycerol in vivo. The ex vivo rabbit eyes recovered to their original state in 60 s after saline-wash treatment, and normal optical coherence tomography images of the posterior segment of the sample eyes proved the self-recovery of in vivo performance. Signal intensities of optical coherence tomography images obtained before and after glycerol treatment were compared to analysis of the effect of optical clearing. To the best of our knowledge, this is the first study for imaging depth extension of optical coherence tomography in both the anterior and posterior segments of eye by using optical clearing agents.

Characteristics of Linear Interstitial Keratitis by In Vivo Confocal Microscopy and Anterior Segment Optical Coherence Tomography

Cornea ◽  
2018 ◽  
Vol 37 (6) ◽  
pp. 785-788
Author(s):  
Aleksandra Petrovic ◽  
Kattayoon Hashemi ◽  
Frank Blaser ◽  
Wolfgang Wild ◽  
George Kymionis
Keyword(s):  
Optical Coherence Tomography ◽  
Confocal Microscopy ◽  
Anterior Segment ◽  
Optical Coherence ◽  
In Vivo Confocal Microscopy ◽  
Interstitial Keratitis

Uveo-scleral outflow pathways after ultrasonic cyclocoagulation in refractory glaucoma: an anterior segment optical coherence tomography and in vivo confocal study

2016 ◽  
Vol 100 (12) ◽  
pp. 1668-1675 ◽  
Author(s):  
Rodolfo Mastropasqua ◽  
Luca Agnifili ◽  
Vincenzo Fasanella ◽  
Lisa Toto ◽  
Lorenza Brescia ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Anterior Segment ◽  
Optical Coherence ◽  
Refractory Glaucoma

scholarly journals Polarization sensitive optical coherence tomography with single input for imaging depth-resolved collagen organizations

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Peijun Tang ◽  
Mitchell A. Kirby ◽  
Nhan Le ◽  
Yuandong Li ◽  
Nicole Zeinstra ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Polarization State ◽  
Transmission Model ◽  
Optical Coherence ◽  
Healthy Human ◽  
Collagen Organization ◽  
Single Input

AbstractCollagen organization plays an important role in maintaining structural integrity and determining tissue function. Polarization-sensitive optical coherence tomography (PSOCT) is a promising noninvasive three-dimensional imaging tool for mapping collagen organization in vivo. While PSOCT systems with multiple polarization inputs have demonstrated the ability to visualize depth-resolved collagen organization, systems, which use a single input polarization state have not yet demonstrated sufficient reconstruction quality. Herein we describe a PSOCT based polarization state transmission model that reveals the depth-dependent polarization state evolution of light backscattered within a birefringent sample. Based on this model, we propose a polarization state tracing method that relies on a discrete differential geometric analysis of the evolution of the polarization state in depth along the Poincare sphere for depth-resolved birefringent imaging using only one single input polarization state. We demonstrate the ability of this method to visualize depth-resolved myocardial architecture in both healthy and infarcted rodent hearts (ex vivo) and collagen structures responsible for skin tension lines at various anatomical locations on the face of a healthy human volunteer (in vivo).

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