Rapid detection of Salmonella Typhimurium in chicken carcass using a SPR biosensor

2005 ◽  
Author(s):  
Shizhou Wang ◽  
Yubin Lan ◽  
Yongguang Yin ◽  
Thirumala R. Dasari
Keyword(s):  
Salmonella Typhimurium ◽  
Rapid Detection ◽  
Spr Biosensor ◽  
Chicken Carcass

Rapid Detection of Salmonella Typhimurium in Chicken Carcass Wash Water Using an Immunoelectrochemical Method

2000 ◽  
Vol 63 (8) ◽  
pp. 1043-1048 ◽  
Author(s):  
YI HUA CHE ◽  
YANBIN LI ◽  
MICHAEL SLAVIK ◽  
DAVID PAUL
Keyword(s):  
Salmonella Typhimurium ◽  
Rapid Detection ◽  
Magnetic Beads ◽  
Immunomagnetic Separation ◽  
Cell Number ◽  
Wash Water ◽  
Carbon Paste ◽  
Buffer Solution ◽  
Magnetic Separator ◽  
Chicken Carcass

An immunoelectrochemical method coupled with immunomagnetic separation was developed for rapid detection of Salmonella Typhimurium in chicken carcass wash water. Samples of chicken carcass wash water were inoculated with Salmonella Typhimurium at different cell numbers. Possible nonspecified inhibitors in the wash water were minimized by filtration and centrifugation. An approximately 9.4% loss of Salmonella cells was found after filtration (P < 0.01). The samples were mixed with anti-Salmonella-coated magnetic beads (ASCMB) and alkaline phosphatase–labeled anti-Salmonella (APLAS) to form ASCMB–Salmonella–APLAS conjugates. The conjugates were separated from the solution using a magnetic separator and then incubated with phenylphosphate substrate to produce phenol. The number of Salmonella was determined by measuring the phenol concentration using an amperometric tyrosinase carbon paste electrode in a flow injection analysis system. Under optimized parameters (1 mM MgCl2, 0.2 μg/ml APLAS, and 1 mM phenylphosphate in pH 7.0 Tris buffer solution), Salmonella Typhimurium in chicken carcass wash water could be identified and enumerated within 2.5 h with a detection limit of 5 × 103 CFU/ml. A linear relationship on a log-log scale was found between Salmonella cell number and the peak current ratio for Salmonella concentrations ranging from 103 to 107 CFU/ml (R2 = 0.963). The peak currents of multibacteria samples, containing Salmonella Typhimurium, Listeria monocytogenes, and Campylobacter jejuni, were not significantly different from Salmonella-only samples (P > 0.01).

Detection of Virulence Plasmid–Encoded Genes in Salmonella Typhimurium and Salmonella Kentucky Isolates Recovered from Commercially Processed Chicken Carcasses

2019 ◽  
Vol 82 (8) ◽  
pp. 1364-1368 ◽  
Author(s):  
RIZWANA TASMIN ◽  
PAUL A. GULIG ◽  
SALINA PARVEEN
Keyword(s):  
United States ◽  
Salmonella Typhimurium ◽  
Virulence Genes ◽  
Salmonella Enterica Serovar Typhimurium ◽  
The United States ◽  
Clinical Isolates ◽  
Virulence Plasmid ◽  
Chicken Carcass ◽  
Serovar Typhimurium ◽  
Salmonella Virulence

ABSTRACT Salmonella enterica serovar Typhimurium is one of the leading causes of nontyphoidal gastroenteritis of humans in the United States. Commercially processed poultry carcasses are frequently contaminated with Salmonella serovar Kentucky in the United States. The aim of the study was to detect the Salmonella virulence plasmid containing the spv genes from Salmonella isolates recovered from commercially processed chicken carcasses. A total of 144 Salmonella isolates (Salmonella Typhimurium, n = 72 and Salmonella Kentucky, n = 72) were used for isolation of plasmids and detection of corresponding virulence genes (spvA, spvB, and spvC). Only four (5.5%) Salmonella Typhimurium isolates tested positive for all three virulence genes and hence were classified as possessing the virulence plasmid. All isolates of Salmonella Kentucky were negative for the virulence plasmid and genes. These results indicate that the virulence plasmid, which is very common among clinical isolates of Typhimurium and other Salmonella serovars (e.g., Enteritidis, Dublin, Choleraesuis, Gallinarum, Pullorum, and Abortusovis), may not be present in a significant portion of commercially processed chicken carcass isolates.

Optical Biosensor for Rapid Detection of Salmonella typhimurium Based on Porous Gold@Platinum Nanocatalysts and a 3D Fluidic Chip

ACS Sensors ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Lingyan Zheng ◽  
Gaozhe Cai ◽  
Wuzhen Qi ◽  
Siyuan Wang ◽  
Maohua Wang ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Rapid Detection ◽  
Optical Biosensor ◽  
Porous Gold ◽  
Gold Platinum

scholarly journals Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206316 ◽  
Author(s):  
Raymond Heymans ◽  
Amir Vila ◽  
Caroliene A. M. van Heerwaarden ◽  
Claudia C. C. Jansen ◽  
Greetje A. A. Castelijn ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Salmonella Enteritidis

Development of a Rapid Response Biosensor for Detection of Salmonella Typhimurium

1999 ◽  
Vol 62 (5) ◽  
pp. 431-437 ◽  
Author(s):  
K. H. SEO ◽  
R. E. BRACKETT ◽  
N. F. HARTMAN ◽  
D. P. CAMPBELL
Keyword(s):  
Detection Limit ◽  
Salmonella Typhimurium ◽  
Foodborne Pathogens ◽  
Reductive Amination ◽  
Reference Channel ◽  
Heat Treated ◽  
Chicken Carcass ◽  
Direct Binding ◽  
Capture Antibody ◽  
Structural Antigen

An integrated optic interferometer for detecting foodborne pathogens was developed. The interferometer is a planar waveguide with two thin antibody-coated channels of immunochemically selective agents that interact with antigen molecules. One channel is coated with antibody to Salmonella as a sample, and the other is coated with human immunoglobulin G as a reference channel by using reductive amination. Salmonella was introduced onto the sensing channels through the flow cell on the channels. Phase shift (π) generated by refractive index variation, as determined by interfering the perturbed sample channel with an unperturbed reference channel and observing the fringe shift, was used for detection. Salmonella Typhimurium (heat-treated or boiled) was detected by binding to antibody against Salmonella common structural antigen immobilized on a silane-derived sensor surface at concentrations in the range of 1 × 105 to 1 × 107 CFU/ml. Salmonella (1 × 107 CFU/ml) mixed with Escherichia coli (1 × 107 CFU/ml) were readily detected without any decrease in sensitivity by the direct assay. Application of a sandwich assay with a second antibody or a gold-conjugated antibody increased the detection limit to 1 × 105 CFU/ml within a 10-min reaction time. Various methods for the immobilization of the capture antibody to the biosensor channels were compared. The greatest binding response was observed in a direct reductive amination method with a long reaction period and increased the detection limit of direct binding of Salmonella antigen to 1 × 104 CFU/ml. The biosensor was able to detect Salmonella Typhimurium in chicken carcass wash fluid originally inoculated at a level of 20 CFU/ml after 12 h of nonselective enrichment. The planar optic biosensor shows promise as a fast, sensitive, reliable, and economical means of detecting food pathogens in the future.

Sign in / Sign up

Export Citation Format

Share Document