An in vitro hemodynamic tissue model to study the variations in flow using near infrared spectroscopy

2005 ◽  
Author(s):  
Raghavender Ranga ◽  
Dheerendra Kashyap ◽  
Khosrow Behbehani ◽  
Hanli Liu
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Tissue Model

Feasibility of near Infrared Spectroscopy Determination of Haemoglobin-Related Blood Levels of Cattle: An in vitro Study

NIR news ◽  
2012 ◽  
Vol 23 (6) ◽  
pp. 13-14 ◽  
Author(s):  
Akifumi Ikehata ◽  
Kunio Sashida ◽  
Shanji Park ◽  
Tsutomu Okura ◽  
Yutaka Terada
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
In Vitro Study ◽  
Vitro Study ◽  
Blood Levels

scholarly journals A New 3D Cultured Liver Chip and Real-Time Monitoring System Based on Microfluidic Technology

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1118
Author(s):  
Yao Zhang ◽  
Ning Yang ◽  
Liangliang Xie ◽  
Fangyu Shu ◽  
Qian Shi ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Monitoring System ◽  
Near Infrared Spectroscopy ◽  
In Vitro Model ◽  
Near Infrared ◽  
Real Time Monitoring ◽  
Microfluidic Technology ◽  
Vitro Model

In vitro models of the liver have a good simulation of the micro-liquid environment inside the human liver and the communication between cell tissues, which provides an important research tool for drug research and liver disease treatment. In this paper, we designed a 3D liver chip and real-time monitoring system based on microfluidic technology. The in vitro model of the liver on the chip was established by the three-dimensional microfluidic chip pipeline and the corresponding microwell array. Meanwhile, the culture medium is continuously injected on the chip, and the electrochemical impedance spectroscopy and near-infrared spectroscopy of the liver chip are recorded and analyzed from day one to day five. When the 3D cultured liver chip in vitro model reached a certain period and stabilized, paracetamol with varying gradients of concentration was applied to the cultured cells for drug resistance testing. The experimental results show that the liver chip and its monitoring system designed in this paper can maintain 100% cell viability of hepatocytes in vitro for a long time. Furthermore, it can meet the requirements of measurement technologies such as electrical impedance measurement and near-infrared spectroscopy in real-time, providing a stable culture platform for the further study of organ chips.

scholarly journals Use of near-infrared spectroscopy for prediction of chemical composition of Tifton 85 grass

2021 ◽  
Vol 42 (3) ◽  
pp. 1287-1302
Author(s):  
Camila Cano Serafim ◽  
◽  
Geisi Loures Guerra ◽  
Ivone Yurika Mizubuti ◽  
Filipe Alexandre Boscaro de Castro ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Chemical Composition ◽  
Spectral Data ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Prediction Models ◽  
Neutral Detergent Fiber ◽  
Mineral Matter ◽  
Data Set

The reduction in the quality, consumption, and digestibility of forage can cause a decrease in animal performance, resulting in losses to the rural producer. Thus, it is important to monitor these characteristics in forage plants to devise strategies or practices that optimize production systems. The aim of this study was to develop and validate prediction models using near-infrared spectroscopy (NIRS) to determine the chemical composition of Tifton 85 grass. Samples of green grass, its morphological structures (whole plant, leaf blade, stem + sheath, and senescent material) and hay, totaling 105 samples were used. Conventional chemical analysis was performed to determine the content of oven-dried samples (ODS), mineral matter (MM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose (CEL), hemicellulose (HEM), and in vitro dry matter digestibility (IVDMD). Subsequently, all the samples were scanned using a Vis-NIR spectrometer to collect spectral data. Principal component analysis (PCA) was applied to the data set, and modified partial least squares was used to correlate reference values to spectral data. The coefficients of determination (R2) were 0.74, 0.85, 0.98, 0.75, 0.85, 0.71, 0.82, 0.77, and 0.93, and the ratio of performance deviations (RPD) obtained were 1.99, 2.71, 6.46, 2.05, 2.58, 3.84, 1.86, 2.35, 2.09, and 3.84 for ODS, MM, CP, NDF, ADF, ADL, CEL, HEM, and IVDMD, respectively. The prediction models obtained, in general, were considered to be of excellent quality, and demonstrated that the determination of the chemical composition of Tifton 85 grass can be performed using NIRS technology, replacing conventional analysis.

Nutritive Evaluation of Olive Tree Leaves by Near-Infrared Spectroscopy: Effect of Soil Contamination and Correction with Spectral Pretreatments

2008 ◽  
Vol 62 (1) ◽  
pp. 51-58 ◽  
Author(s):  
V. M. Fernández-Cabanás ◽  
A. Garrido-Varo ◽  
M. Delgado-Pertiñez ◽  
A. Gómez-Cabrera
Keyword(s):  
Infrared Spectroscopy ◽  
Soil Contamination ◽  
Near Infrared Spectroscopy ◽  
Nutritive Value ◽  
Near Infrared ◽  
Scatter Correction ◽  
Olive Tree ◽  
Curvature Effects ◽  
Productive Systems

Olive leaves obtained as a byproduct in the Mediterranean region could play an important role in the nutrition of extensive ruminant systems. However, the reported variation in their nutritive value, among other reasons due to discrepancies in mineral content, is considered an important obstacle for their common use. Near-infrared spectroscopy (NIRS) could fulfill the requirements of these productive systems, providing analytical information in a rapid and economic way. In this work, the effect of soil contamination on NIR spectra has been studied, as well as its correction with some of the most commonly used spectral pretreatments (derivatives, multiplicative scatter correction, auto scaling, detrending, and a combination of the last two transforms). Effects were evaluated by visual inspection of the transformed spectra and comparison of the calibration statistics obtained to estimate acid insoluble ash and total ash contents and in vitro pepsin cellulase digestibility of organic and dry matter. The incidence of spectral curvature effects caused by soil contamination that can be conveniently corrected with pretreatments such as derivatives was confirmed.

Nutritional characteristics and in vitro digestibility by near-infrared spectroscopy of local and hybrid napiergrass varieties grown in rain-fed and irrigated conditions

2014 ◽  
Vol 54 (10) ◽  
pp. 1775
Author(s):  
U. P. Tiwari ◽  
B. Turano ◽  
R. Jha
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Nutritional Value ◽  
Nutritive Value ◽  
Near Infrared ◽  
In Vitro Digestibility ◽  
Neutral Detergent Fibre ◽  
Drought Tolerant ◽  
Animal Feeding

Napiergrass can produce large amounts of biomass and its nutritive value has a significant effect on its effectiveness for animal production. However, temperature and drought stress limit its productivity. Drought-tolerant pearl millet × napiergrass hybrid (PMN) varieties were developed and produce high biomass yields. The nutritional content and digestibility of PMN is not well known, which limits its use in animal feeding. It was hypothesised that PMN hybrids are more drought tolerant and have higher nutritive value than napiergrass varieties. Four napiergrass varieties (Bana grass, Mott, MB4, and N51) and four PMN (PMN2, PMN3, 5344, 4604) were tested with or without irrigation treatment in a strip-plot design, with the objective of evaluating the nutritional value and in vitro digestibility of PMN hybrids and napiergrass. The forages were harvested on Day 110 of planting. Samples were hand chopped, oven-dried, ground to pass through a 1-mm screen and analysed for their nutrient content and in vitro digestibility using near-infrared spectroscopy. Dry matter (DM) content of PMN2 (24.3%) and PMN3 (22.9%) was significantly higher (P < 0.05) than 5344, Bana grass and N51 napiergrass varieties. No differences (P > 0.05) in acid detergent fibre, neutral detergent fibre, crude protein and metabolisable energy were found among napiergrass varieties. With no effect (P > 0.05) of irrigation, lignin content was highest (P < 0.08) in 4604 (8.2%) and lowest in 5344 (5.2%). Starch was highest (P < 0.05) in irrigated MB4 than both irrigated and non-irrigated 4604. Non-fibre carbohydrate content was highest (P < 0.05) in PMN2 (12.8%) than MB4 (8.7%). The in vitro true digestibility was significantly higher (P < 0.05) in 5344 and Bana grass (70.0% and 68.0% of DM, respectively), than PMN3 (54.5%). Rate of digestion was significantly higher (P < 0.05) in 5344 (4.9%/h) than PMN2 (2.7%/h), others were in between. Neutral detergent fibre digestibility (NDFD) of 5344 and Bana grass (56.7% and 53.2% of neutral detergent fibre, respectively) was significantly higher (P < 0.05) than PMN2 (38.0%). Although no effect of irrigation was observed, there was an interaction (P < 0.05) between variety and irrigation on neutral detergent fibre digestibility of napiergrass varieties. In conclusion, among four PMN varieties tested, PMN3 and 5344 has higher nutritional value and in vitro digestibility than PMN2 and 4604 even when grown in non-irrigated condition. Thus, PMN3 and 5344 is the preferred napiergrass variety for animal feeding, even in rain-fed farming conditions.

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