Nutritional characteristics and in vitro digestibility by near-infrared spectroscopy of local and hybrid napiergrass varieties grown in rain-fed and irrigated conditions

2014 ◽  
Vol 54 (10) ◽  
pp. 1775
Author(s):  
U. P. Tiwari ◽  
B. Turano ◽  
R. Jha

Napiergrass can produce large amounts of biomass and its nutritive value has a significant effect on its effectiveness for animal production. However, temperature and drought stress limit its productivity. Drought-tolerant pearl millet × napiergrass hybrid (PMN) varieties were developed and produce high biomass yields. The nutritional content and digestibility of PMN is not well known, which limits its use in animal feeding. It was hypothesised that PMN hybrids are more drought tolerant and have higher nutritive value than napiergrass varieties. Four napiergrass varieties (Bana grass, Mott, MB4, and N51) and four PMN (PMN2, PMN3, 5344, 4604) were tested with or without irrigation treatment in a strip-plot design, with the objective of evaluating the nutritional value and in vitro digestibility of PMN hybrids and napiergrass. The forages were harvested on Day 110 of planting. Samples were hand chopped, oven-dried, ground to pass through a 1-mm screen and analysed for their nutrient content and in vitro digestibility using near-infrared spectroscopy. Dry matter (DM) content of PMN2 (24.3%) and PMN3 (22.9%) was significantly higher (P < 0.05) than 5344, Bana grass and N51 napiergrass varieties. No differences (P > 0.05) in acid detergent fibre, neutral detergent fibre, crude protein and metabolisable energy were found among napiergrass varieties. With no effect (P > 0.05) of irrigation, lignin content was highest (P < 0.08) in 4604 (8.2%) and lowest in 5344 (5.2%). Starch was highest (P < 0.05) in irrigated MB4 than both irrigated and non-irrigated 4604. Non-fibre carbohydrate content was highest (P < 0.05) in PMN2 (12.8%) than MB4 (8.7%). The in vitro true digestibility was significantly higher (P < 0.05) in 5344 and Bana grass (70.0% and 68.0% of DM, respectively), than PMN3 (54.5%). Rate of digestion was significantly higher (P < 0.05) in 5344 (4.9%/h) than PMN2 (2.7%/h), others were in between. Neutral detergent fibre digestibility (NDFD) of 5344 and Bana grass (56.7% and 53.2% of neutral detergent fibre, respectively) was significantly higher (P < 0.05) than PMN2 (38.0%). Although no effect of irrigation was observed, there was an interaction (P < 0.05) between variety and irrigation on neutral detergent fibre digestibility of napiergrass varieties. In conclusion, among four PMN varieties tested, PMN3 and 5344 has higher nutritional value and in vitro digestibility than PMN2 and 4604 even when grown in non-irrigated condition. Thus, PMN3 and 5344 is the preferred napiergrass variety for animal feeding, even in rain-fed farming conditions.

2008 ◽  
Vol 62 (1) ◽  
pp. 51-58 ◽  
Author(s):  
V. M. Fernández-Cabanás ◽  
A. Garrido-Varo ◽  
M. Delgado-Pertiñez ◽  
A. Gómez-Cabrera

Olive leaves obtained as a byproduct in the Mediterranean region could play an important role in the nutrition of extensive ruminant systems. However, the reported variation in their nutritive value, among other reasons due to discrepancies in mineral content, is considered an important obstacle for their common use. Near-infrared spectroscopy (NIRS) could fulfill the requirements of these productive systems, providing analytical information in a rapid and economic way. In this work, the effect of soil contamination on NIR spectra has been studied, as well as its correction with some of the most commonly used spectral pretreatments (derivatives, multiplicative scatter correction, auto scaling, detrending, and a combination of the last two transforms). Effects were evaluated by visual inspection of the transformed spectra and comparison of the calibration statistics obtained to estimate acid insoluble ash and total ash contents and in vitro pepsin cellulase digestibility of organic and dry matter. The incidence of spectral curvature effects caused by soil contamination that can be conveniently corrected with pretreatments such as derivatives was confirmed.


2016 ◽  
Vol 56 (9) ◽  
pp. 1504 ◽  
Author(s):  
J. P. Keim ◽  
H. Charles ◽  
D. Alomar

An important constraint of in situ degradability studies is the need to analyse a high number of samples and often with insufficient amount of residue, especially after the longer incubations of high-quality forages, that impede the study of more than one nutritional component. Near-infrared spectroscopy (NIRS) has been established as a reliable method for predicting composition of many entities, including forages and other animal feedstuffs. The objective of this work was to evaluate the potential of NIRS for predicting the crude protein (CP) and neutral detergent fibre (NDF) concentration in rumen incubation residues of permanent and sown temperate pastures in a vegetative stage. In situ residues (n = 236) from four swards were scanned for their visible-NIR spectra and analysed for CP and NDF. Selected equations developed by partial least-squares multivariate regression presented high coefficients of determination (CP = 0.99, NDF = 0.95) and low standard errors (CP = 4.17 g/kg, NDF = 7.91 g/kg) in cross-validation. These errors compare favourably to the average concentrations of CP and NDF (146.5 and 711.2 g/kg, respectively) and represent a low fraction of their standard deviation (CP = 38.2 g/kg, NDF = 34.4 g/kg). An external validation was not as successful, with R2 of 0.83 and 0.82 and a standard error of prediction of 14.8 and 15.2 g/kg, for CP and NDF, respectively. It is concluded that NIRS has the potential to predict CP and NDF of in situ incubation residues of leafy pastures typical of humid temperate zones, but more robust calibrations should be developed.


Sensors ◽  
2022 ◽  
Vol 22 (2) ◽  
pp. 658
Author(s):  
Matthew F. Digman ◽  
Jerry H. Cherney ◽  
Debbie J. R. Cherney

Advanced manufacturing techniques have enabled low-cost, on-chip spectrometers. Little research exists, however, on their performance relative to the state of technology systems. The present study compares the utility of a benchtop FOSS NIRSystems 6500 (FOSS) to a handheld NeoSpectra-Scanner (NEO) to develop models that predict the composition of dried and ground grass, and alfalfa forages. Mixed-species prediction models were developed for several forage constituents, and performance was assessed using an independent dataset. Prediction models developed with spectra from the FOSS instrument had a standard error of prediction (SEP, % DM) of 1.4, 1.8, 3.3, 1.0, 0.42, and 1.3, for neutral detergent fiber (NDF), true in vitro digestibility (IVTD), neutral detergent fiber digestibility (NDFD), acid detergent fiber (ADF), acid detergent lignin (ADL), and crude protein (CP), respectively. The R2P for these models ranged from 0.90 to 0.97. Models developed with the NEO resulted in an average increase in SEP of 0.14 and an average decrease in R2P of 0.002.


NIR news ◽  
2012 ◽  
Vol 23 (6) ◽  
pp. 13-14 ◽  
Author(s):  
Akifumi Ikehata ◽  
Kunio Sashida ◽  
Shanji Park ◽  
Tsutomu Okura ◽  
Yutaka Terada

2005 ◽  
Vol 4 (sup2) ◽  
pp. 141-143
Author(s):  
S. Colombini ◽  
M. Confalonieri ◽  
G. Borreani ◽  
E. Tabacco ◽  
P.G. Peiretti ◽  
...  

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1118
Author(s):  
Yao Zhang ◽  
Ning Yang ◽  
Liangliang Xie ◽  
Fangyu Shu ◽  
Qian Shi ◽  
...  

In vitro models of the liver have a good simulation of the micro-liquid environment inside the human liver and the communication between cell tissues, which provides an important research tool for drug research and liver disease treatment. In this paper, we designed a 3D liver chip and real-time monitoring system based on microfluidic technology. The in vitro model of the liver on the chip was established by the three-dimensional microfluidic chip pipeline and the corresponding microwell array. Meanwhile, the culture medium is continuously injected on the chip, and the electrochemical impedance spectroscopy and near-infrared spectroscopy of the liver chip are recorded and analyzed from day one to day five. When the 3D cultured liver chip in vitro model reached a certain period and stabilized, paracetamol with varying gradients of concentration was applied to the cultured cells for drug resistance testing. The experimental results show that the liver chip and its monitoring system designed in this paper can maintain 100% cell viability of hepatocytes in vitro for a long time. Furthermore, it can meet the requirements of measurement technologies such as electrical impedance measurement and near-infrared spectroscopy in real-time, providing a stable culture platform for the further study of organ chips.


2005 ◽  
Author(s):  
Raghavender Ranga ◽  
Dheerendra Kashyap ◽  
Khosrow Behbehani ◽  
Hanli Liu

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