scholarly journals Microrheology using dual-beam optical tweezers and ultrasensitive force measurements

2005 ◽  
Author(s):  
Gregor G. Knoener ◽  
Simon J. W. Parkin ◽  
Norman R. Heckenberg ◽  
Halina Rubinsztein-Dunlop
Keyword(s):  
Optical Tweezers ◽  
Force Measurements ◽  
Dual Beam

scholarly journals Hemodynamic forces can be accurately measured in vivo with optical tweezers

2017 ◽  
Author(s):  
Sébastien Harlepp ◽  
Fabrice Thalmann ◽  
Gautier Follain ◽  
Jacky G. Goetz
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Accurate Determination ◽  
Force Measurements ◽  
Hemodynamic Forces ◽  
Non Invasive ◽  
Drag Forces ◽  
Measured Flow ◽  
Living Organisms

AbstractForce sensing and generation at the tissular and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows non-invasive probing of pico-Newton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles rely in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here, we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether, this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.

Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

2001 ◽  
Author(s):  
Bahman Anvari ◽  
Zhiwei Li ◽  
Masayoshi Takashima ◽  
Peter Brecht ◽  
Jorge H. Torres ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Force Measurements

scholarly journals Optical Tweezers for Quantitative Force Measurements and Live Cell Experiments

2014 ◽  
Vol 22 (4) ◽  
pp. 24-31 ◽  
Author(s):  
Philipp Rauch ◽  
Torsten Jähnke
Keyword(s):  
Optical Tweezers ◽  
Live Cell ◽  
Force Measurements

Precision force measurements with optical tweezers

2020 ◽  
Author(s):  
Declan Armstrong ◽  
Alexander B. Stilgoe ◽  
Timo Nieminen ◽  
Halina Rubinsztein-Dunlop
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

Optical tweezers force measurements to study parasites chemotaxis

2009 ◽  
Author(s):  
A. A. de Thomaz ◽  
L. Y. Pozzo ◽  
A. Fontes ◽  
D. B. Almeida ◽  
C. V. Stahl ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

scholarly journals Single molecular elasticity of spectrin as studies by dual-beam optical tweezers

1999 ◽  
Vol 39 (supplement) ◽  
pp. S94
Author(s):  
M. Tomishige ◽  
S. Manno ◽  
Y. Takakuwa ◽  
A. Kusumi
Keyword(s):  
Optical Tweezers ◽  
Dual Beam

scholarly journals Calibration of Optical Tweezers for In Vivo Force Measurements: How do Different Approaches Compare?

2014 ◽  
Vol 107 (6) ◽  
pp. 1474-1484 ◽  
Author(s):  
Yonggun Jun ◽  
Suvranta K. Tripathy ◽  
Babu R.J. Narayanareddy ◽  
Michelle K. Mattson-Hoss ◽  
Steven P. Gross
Keyword(s):  
Optical Tweezers ◽  
Force Measurements

Displacement and Force Measurements with Quadrant Photodetector in Optical Tweezers

2003 ◽  
Vol 20 (6) ◽  
pp. 950-952 ◽  
Author(s):  
Guo Hong-Lian ◽  
Liu Chun-Xiang ◽  
Li Zhao-Lin ◽  
Duan Jian-Fa ◽  
Han Xue-Hai ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Force Measurements ◽  
Quadrant Photodetector

scholarly journals Precise control and measurement of solid–liquid interfacial temperature and viscosity using dual-beam femtosecond optical tweezers in the condensed phase

2016 ◽  
Vol 18 (37) ◽  
pp. 25823-25830 ◽  
Author(s):  
Dipankar Mondal ◽  
Paresh Mathur ◽  
Debabrata Goswami
Keyword(s):  
Optical Tweezers ◽  
Condensed Phase ◽  
Precise Control ◽  
Dual Beam ◽  
Novel Method ◽  
Interfacial Temperature ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
And Control

We present a novel method of microrheology based on femtosecond optical tweezers, which in turn enables us to directly measure and controlin situtemperature at microscale volumes at the solid–liquid interface.

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