Time-resolved ratio method for multicomponent fluorescence pattern analysis using a fluorescence lifetime imaging system

1992 ◽  
Author(s):  
Xue F. Wang ◽  
Takeshi Uchida ◽  
Shigeo Minami
Keyword(s):  
Fluorescence Lifetime ◽  
Pattern Analysis ◽  
Imaging System ◽  
Ratio Method ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Fluorescence Pattern ◽  
Lifetime Imaging

scholarly journals The biochemical resolving power of fluorescence lifetime imaging

2021 ◽  
Author(s):  
Andrew L. Trinh ◽  
Alessandro Esposito
Keyword(s):  
Fluorescence Lifetime ◽  
Resolving Power ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Instrument Response Function ◽  
Lifetime Imaging ◽  
Use Of Time ◽  
Single Living Cells ◽  
Microscopy Techniques

AbstractA deeper understanding of spatial resolution in microscopy fostered a technological revolution that is now permitting us to investigate the structure of the cell with nanometer resolution. Although fluorescence microscopy techniques enable scientists to investigate both the structure and biochemistry of the cell, the biochemical resolving power of a microscope is a physical quantity that is not well-defined or studied. To overcome this limitation, we carried out a theoretical investigation of the biochemical resolving power in fluorescence lifetime imaging microscopy, one of the most effective tools to investigate biochemistry in single living cells. With the theoretical analysis of information theory and Monte Carlo simulations, we describe how the ‘biochemical resolving power’ in time-resolved sensing depends on instrument specifications. We unravel common misunderstandings on the role of the instrument response function and provide theoretical insights that have significant practical implications in the design and use of time-resolved instrumentation.

Fluorescence Pattern Analysis Based on the Time-Resolved Ratio Method

1991 ◽  
Vol 45 (4) ◽  
pp. 560-565 ◽  
Author(s):  
Xue Feng Wang ◽  
Teruo Uchida ◽  
Muneo Maeshima ◽  
Shigeo Minami
Keyword(s):  
Pattern Analysis ◽  
Ratio Method ◽  
Time Resolved ◽  
Fluorescence Pattern

A Time-Resolved Four-Tap Lock-In Pixel CMOS Image Sensor for Real-Time Fluorescence Lifetime Imaging Microscopy

2018 ◽  
Vol 53 (8) ◽  
pp. 2319-2330 ◽  
Author(s):  
Min-Woong Seo ◽  
Yuya Shirakawa ◽  
Yoshimasa Kawata ◽  
Keiichiro Kagawa ◽  
Keita Yasutomi ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Lifetime ◽  
Cmos Image Sensor ◽  
Image Sensor ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Lifetime Imaging ◽  
Lock In

Fluorescence Lifetime Imaging and Fourier Transform Infrared Spectroscopy of Michelangelo's David

2005 ◽  
Vol 59 (9) ◽  
pp. 1174-1181 ◽  
Author(s):  
Daniela Comelli ◽  
Gianluca Valentini ◽  
Rinaldo Cubeddu ◽  
Lucia Toniolo
Keyword(s):  
Fourier Transform ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Inorganic Compounds ◽  
Fluorescence Emission ◽  
Chemical Characterization ◽  
Fourier Transform Infrared ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Analytical Measurements

We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.

Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

2002 ◽  
Author(s):  
Michael Wahl ◽  
Hans-Juergen Rahn ◽  
Uwe Ortmann ◽  
Rainer Erdmann ◽  
Martin Boehmer ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

All-solid-state diode-pumped fluorescence lifetime imaging system for biomedicine and microscopy

1999 ◽  
Author(s):  
M. J. Cole ◽  
K. Dowling ◽  
R. Jones ◽  
D. Parsons-Karavassilis ◽  
P. M. W. French ◽  
...  
Keyword(s):  
Solid State ◽  
Fluorescence Lifetime ◽  
Imaging System ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Diode Pumped
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