Compact whole-body fluorescent imaging of nude mice bearing EGFP expressing tumor

2005 ◽  
Author(s):  
Yanping Chen ◽  
Tao Xiong ◽  
Jun Chu ◽  
Li Yu ◽  
Shaoqun Zeng ◽  
...  
Keyword(s):  
Nude Mice ◽  
Fluorescent Imaging ◽  
Whole Body

scholarly journals Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Wei Xin Cai ◽  
Li Wu Zheng ◽  
Li Ma ◽  
Hong Zhang Huang ◽  
Ru Qing Yu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Nude Mice ◽  
Tongue Cancer ◽  
Cancer Cell Lines ◽  
Fluorescent Imaging ◽  
Cell Phenotype ◽  
Human Tongue

Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice. A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n=8). A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back. Validity assessment of the labelled cancer cells’ tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection. The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells. Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging. UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns. Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.

Influence of route of administration on [ 3 H]-camptothecin distribution and tumor uptake in CASE-bearing nude mice: whole-body autoradiographic studies

1996 ◽  
Vol 39 (1-2) ◽  
pp. 122-130 ◽  
Author(s):  
A. E. Ahmed ◽  
Sam Jacob ◽  
Beppino C. Giovanella ◽  
Anthony J. Kozielski ◽  
John S. Stehlin Jr. ◽  
...  
Keyword(s):  
Nude Mice ◽  
Route Of Administration ◽  
Whole Body ◽  
Tumor Uptake

Enhancement of Tumor Contrast on Radioimmunoscans by Using Mixtures of Monoclonal Antibody F(ab’)2 Fragments

1986 ◽  
Vol 25 (06) ◽  
pp. 216-219 ◽  
Author(s):  
A. Alavi ◽  
H. Koprowski ◽  
D. Herlyn ◽  
D. L. Munz
Keyword(s):  
Monoclonal Antibody ◽  
Gastrointestinal Tract ◽  
Nude Mice ◽  
Half Life ◽  
The Body ◽  
Whole Body ◽  
Antigenic Determinants ◽  
Body Images ◽  
Biological Half Life ◽  
Adenocarcinoma Cells

F(ab’)2 fragments of MAbs GA 73-3 (IgG 2a) and CO 29.11 (IgG 1), which detect distinct antigenic determinants on adenocarcinoma cells of the gastrointestinal tract, were labeled with 131I using the iodogen method. 41 nude mice bearing SW-948 CRC tumors were injected either with a mixture of 100 ¼Ci (11 ¼g) each (n = 9) of the two 131l-F(ab’)2 fragments or with either fragment alone at various doses (each group consisting of 8 mice): GA 73-3,100 ¼Ci (11 ¼g) and 200 ¼Ci (25 ¼g); CO 29.11,100 ¼Ci (11 ¼g) and 200 ¼Ci (26 ¼g). Whole-body images of the mice were obtained daily for up to six days after injection. Ratios of cpm/pixel in the tumor to those in the rest of the body (rob), representing tumor contrast, were significantly (p <0.05) higher in the group of mice injected with the mixture (3.9 ± 1.5) as compared to those given 100 or 200 jiCi of either fragment separately. The biological half-life (T1/2 biol) of the mixture (44.7 ± 14.5 h) in the CRC tumors was significantly (p <0.05) longer than T1/2 biol determined in the groups given either fragment alone. Tv bioL in the rob was similar in all groups of mice examined.

scholarly journals Far-red fluorescent tags for protein imaging in living tissues

2009 ◽  
Vol 418 (3) ◽  
pp. 567-574 ◽  
Author(s):  
Dmitry Shcherbo ◽  
Christopher S. Murphy ◽  
Galina V. Ermakova ◽  
Elena A. Solovieva ◽  
Tatiana V. Chepurnykh ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Protein Localization ◽  
Imaging Techniques ◽  
Fluorescent Imaging ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Fluorescent Tags ◽  
Living Tissues ◽  
Fluorescent Tag

A vast colour palette of monomeric fluorescent proteins has been developed to investigate protein localization, motility and interactions. However, low brightness has remained a problem in far-red variants, which hampers multicolour labelling and whole-body imaging techniques. In the present paper, we report mKate2, a monomeric far-red fluorescent protein that is almost 3-fold brighter than the previously reported mKate and is 10-fold brighter than mPlum. The high-brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues. We also report tdKatushka2, a tandem far-red tag that performs well in fusions, provides 4-fold brighter near-IR fluorescence compared with mRaspberry or mCherry, and is 20-fold brighter than mPlum. Together, monomeric mKate2 and pseudo-monomeric tdKatushka2 represent the next generation of extra-bright far-red fluorescent probes offering novel possibilities for fluorescent imaging of proteins in living cells and animals.

scholarly journals Radiolabeled fragments of monoclonal antibodies against carcinoembryonic antigen for localization of human colon carcinoma grafted into nude mice.

1983 ◽  
Vol 158 (2) ◽  
pp. 413-427 ◽  
Author(s):  
F Buchegger ◽  
C M Haskell ◽  
M Schreyer ◽  
B R Scazziga ◽  
S Randin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Colon Carcinoma ◽  
Direct Measurement ◽  
Nude Mice ◽  
Human Colon ◽  
Whole Body ◽  
Fab Fragments ◽  
Human Colon Carcinoma ◽  
Apical Side

Four monoclonal antibodies against carcinoembryonic antigen (CEA) have been selected from 32 hybrids that produce antibodies against this antigen, by the criteria of high affinity for CEA and low cross-reactivity with granulocyte glycoprotein(s). The specificity of tumor localization in vivo of the four MAb, and their F(ab')2 and Fab fragments was compared in nude mice bearing grafts of a serially transplanted, CEA-producing, human colon carcinoma. The distribution of radiolabeled MAb and their fragments after intravenous injection was analyzed by direct measurement of radioactivity in tumor and normal organs, as well as by whole-body scanning and by autoradiography of tumor sections. Paired labeling experiments, in which 131I-labeled antibody or fragments and 125I-labeled control IgG are injected simultaneously, were undertaken to determine the relative tumor uptakes of each labeled protein. The tumor antibody uptake divided by that of control IgG defines the specificity index of localization. Tumor antibody uptakes (as compared with the whole mouse), ranging between 7 and 15, and specificity indices ranging between 3.4 and 6.8, were obtained with the four intact MAb at day 4-5 after injection. With F(ab')2 fragments of the four MAb, at day 3, the tumor antibody uptakes ranged between 12 and 24 and the specificity indices between 5.3 and 8.2. With the Fab fragments prepared from the two most promising MAb, the antibody uptakes reached values of 34 and 82 at day 2-3 and the specificity indices were as high as 12 and 19. The scanning results paralleled those obtained by direct measurement of radioactivity. With intact MAb, tumor grafts of 0.5-1 g gave very contrasted positive scans 3 d after injection. Using MAb fragments, tumors of smaller size were detectable earlier. The best results were obtained with Fab fragments of MAb 35, which gave clear detections of tumors weighing only 0.1 g as early as 48 h after injection. Autoradiographs of tumor sections from mice injected with 125I-labeled MAb demonstrated that the radioactivity was localized in the tumor tissues and not in the stromal connective tissue of mouse origin. The highest radioactivity concentration was localized in areas known to contain CEA such as the pseudolumen of glands and the apical side of carcinoma cells. The penetration of radioactivity in the central part of tumor nodules and the pseudolumen appeared to be increased with the use of MAb fragments.

Optoacoustic 3D whole-body tomography: experiments in nude mice

2009 ◽  
Author(s):  
Hans-Peter Brecht ◽  
Richard Su ◽  
Matt Fronheiser ◽  
Sergey A. Ermilov ◽  
André Conjusteau ◽  
...  
Keyword(s):  
Nude Mice ◽  
Whole Body

scholarly journals Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 300
Author(s):  
Wendy Bernhard ◽  
Kris Barreto ◽  
Ayman El-Sayed ◽  
John DeCoteau ◽  
C. Ronald Geyer
Keyword(s):  
Immune Response ◽  
Immune Cells ◽  
Near Infrared ◽  
Immune Cell ◽  
Cell Types ◽  
Blood Analysis ◽  
Fluorescent Imaging ◽  
Whole Body ◽  
Non Invasive ◽  
Xenograft Models

Tracking immune responses is complex due to the mixture of cell types, variability in cell populations, and the dynamic environment. Tissue biopsies and blood analysis can identify infiltrating and circulating immune cells; however, due to the dynamic nature of the immune response, these are prone to sampling errors. Non-invasive targeted molecular imaging provides a method to monitor immune response, which has advantages of providing whole-body images, being non-invasive, and allowing longitudinal monitoring. Three non-specific Fc-containing proteins were labeled with near-infrared dye IRDye800CW and used as imaging probes to assess tumor-infiltrating immune cells in FaDu and A-431 xenograft models. We showed that Fc domains localize to tumors and are visible by fluorescent imaging. This tumor localization appears to be based on binding tumor-associated immune cells and some xenografts showed higher fluorescent signals than others. The Fc domain alone bound to different human immune cell types. The Fc domain can be a valuable research tool to study innate immune response.

99mTechnetium- or Cy7-Labeled Fab(Tocilizumab) as Potential Multiple Myeloma Imaging Agents

2021 ◽  
Vol 21 ◽  
Author(s):  
Ximena Camacho ◽  
Carolina Perroni ◽  
Camila L. Machado ◽  
Camila de Godoi Carneiro ◽  
Mara de Souza Junqueira ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Cell Lines ◽  
Tissue Sample ◽  
Surgical Excision ◽  
Fluorescent Imaging ◽  
Whole Body ◽  
Imaging Agents ◽  
Tumor Identification

Background: Multiple myeloma (MM) is malignant hematologic disorder and the second most common blood cancer. Interleukin-6 (IL-6) has been identified as a crucial factor for proliferation and survival of MM cells and the overexpression of IL-6 receptor is being studied as a molecular target for therapeutic and diagnostic use in myelomas and other comorbidities. Tocilizumab is a humanized monoclonal antibody that binds IL-6R. Objective: We aim to label and evaluate Fab(Tocilizumab) with 99mTechnetium or Cy7 as potential MM imaging agents. Methods: IL-6R distribution was analyzed by laser confocal microscopy (LCM) in MM cell lines. Fab(Tocilizumab) were produced by digestion of Tocilizumab with papain for 24 h at 37 °C, derivatized with NHS-HYNIC-Tfa and radiolabeled with 99mTc. Radiochemical stability and in vitro cell assays were evaluated. Biodistribution and SPECT/CT were performed. Also, Fab(Tocilizumab) was labeled with Cy7 for in vivo fluorescence imaging up to 72 h. Results: LCM analysis demonstrates IL-6R distribution on MM cell lines. Incubation with papain resulted in complete digestion of Tocilizumab and exhibited a good purity and homogeneity. Radiolabeling with 99mTc via NHS-HYNIC-Tfa was found to be fast, easy, reproducible and stable, revealing high radiochemical purity and without interfering with IL-6R recognition. Biodistribution and SPECT/CT studies showed a quick blood clearance and significant kidney and MM engrafted tumor uptake. Cy7-Fab(Tocilizumab) fluorescent imaging allowed MM1S tumor identification up to 72 h p.i. Conclusion: These new molecular imaging agents could potentially be used in the clinical setting for staging and follow up of MM through radioactive whole-body IL-6R expression visualization in vivo. The fluorescent version could be used for tissue sample evaluation and to guide the surgical excision if necessary.

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