scholarly journals Human non-Hodgkin's malignant lymphomas serially transplanted in nude mice conditioned with whole-body irradiation

1989 ◽  
Vol 59 (3) ◽  
pp. 356-360 ◽  
Author(s):  
T Igarashi ◽  
K Oka ◽  
T Miyamoto
Keyword(s):  
Nude Mice ◽  
Whole Body ◽  
Malignant Lymphomas

Influence of route of administration on [ 3 H]-camptothecin distribution and tumor uptake in CASE-bearing nude mice: whole-body autoradiographic studies

1996 ◽  
Vol 39 (1-2) ◽  
pp. 122-130 ◽  
Author(s):  
A. E. Ahmed ◽  
Sam Jacob ◽  
Beppino C. Giovanella ◽  
Anthony J. Kozielski ◽  
John S. Stehlin Jr. ◽  
...  
Keyword(s):  
Nude Mice ◽  
Route Of Administration ◽  
Whole Body ◽  
Tumor Uptake

Enhancement of Tumor Contrast on Radioimmunoscans by Using Mixtures of Monoclonal Antibody F(ab’)2 Fragments

1986 ◽  
Vol 25 (06) ◽  
pp. 216-219 ◽  
Author(s):  
A. Alavi ◽  
H. Koprowski ◽  
D. Herlyn ◽  
D. L. Munz
Keyword(s):  
Monoclonal Antibody ◽  
Gastrointestinal Tract ◽  
Nude Mice ◽  
Half Life ◽  
The Body ◽  
Whole Body ◽  
Antigenic Determinants ◽  
Body Images ◽  
Biological Half Life ◽  
Adenocarcinoma Cells

F(ab’)2 fragments of MAbs GA 73-3 (IgG 2a) and CO 29.11 (IgG 1), which detect distinct antigenic determinants on adenocarcinoma cells of the gastrointestinal tract, were labeled with 131I using the iodogen method. 41 nude mice bearing SW-948 CRC tumors were injected either with a mixture of 100 ¼Ci (11 ¼g) each (n = 9) of the two 131l-F(ab’)2 fragments or with either fragment alone at various doses (each group consisting of 8 mice): GA 73-3,100 ¼Ci (11 ¼g) and 200 ¼Ci (25 ¼g); CO 29.11,100 ¼Ci (11 ¼g) and 200 ¼Ci (26 ¼g). Whole-body images of the mice were obtained daily for up to six days after injection. Ratios of cpm/pixel in the tumor to those in the rest of the body (rob), representing tumor contrast, were significantly (p <0.05) higher in the group of mice injected with the mixture (3.9 ± 1.5) as compared to those given 100 or 200 jiCi of either fragment separately. The biological half-life (T1/2 biol) of the mixture (44.7 ± 14.5 h) in the CRC tumors was significantly (p <0.05) longer than T1/2 biol determined in the groups given either fragment alone. Tv bioL in the rob was similar in all groups of mice examined.

Compact whole-body fluorescent imaging of nude mice bearing EGFP expressing tumor

2005 ◽  
Author(s):  
Yanping Chen ◽  
Tao Xiong ◽  
Jun Chu ◽  
Li Yu ◽  
Shaoqun Zeng ◽  
...  
Keyword(s):  
Nude Mice ◽  
Fluorescent Imaging ◽  
Whole Body

scholarly journals Radiolabeled fragments of monoclonal antibodies against carcinoembryonic antigen for localization of human colon carcinoma grafted into nude mice.

1983 ◽  
Vol 158 (2) ◽  
pp. 413-427 ◽  
Author(s):  
F Buchegger ◽  
C M Haskell ◽  
M Schreyer ◽  
B R Scazziga ◽  
S Randin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carcinoembryonic Antigen ◽  
Colon Carcinoma ◽  
Direct Measurement ◽  
Nude Mice ◽  
Human Colon ◽  
Whole Body ◽  
Fab Fragments ◽  
Human Colon Carcinoma ◽  
Apical Side

Four monoclonal antibodies against carcinoembryonic antigen (CEA) have been selected from 32 hybrids that produce antibodies against this antigen, by the criteria of high affinity for CEA and low cross-reactivity with granulocyte glycoprotein(s). The specificity of tumor localization in vivo of the four MAb, and their F(ab')2 and Fab fragments was compared in nude mice bearing grafts of a serially transplanted, CEA-producing, human colon carcinoma. The distribution of radiolabeled MAb and their fragments after intravenous injection was analyzed by direct measurement of radioactivity in tumor and normal organs, as well as by whole-body scanning and by autoradiography of tumor sections. Paired labeling experiments, in which 131I-labeled antibody or fragments and 125I-labeled control IgG are injected simultaneously, were undertaken to determine the relative tumor uptakes of each labeled protein. The tumor antibody uptake divided by that of control IgG defines the specificity index of localization. Tumor antibody uptakes (as compared with the whole mouse), ranging between 7 and 15, and specificity indices ranging between 3.4 and 6.8, were obtained with the four intact MAb at day 4-5 after injection. With F(ab')2 fragments of the four MAb, at day 3, the tumor antibody uptakes ranged between 12 and 24 and the specificity indices between 5.3 and 8.2. With the Fab fragments prepared from the two most promising MAb, the antibody uptakes reached values of 34 and 82 at day 2-3 and the specificity indices were as high as 12 and 19. The scanning results paralleled those obtained by direct measurement of radioactivity. With intact MAb, tumor grafts of 0.5-1 g gave very contrasted positive scans 3 d after injection. Using MAb fragments, tumors of smaller size were detectable earlier. The best results were obtained with Fab fragments of MAb 35, which gave clear detections of tumors weighing only 0.1 g as early as 48 h after injection. Autoradiographs of tumor sections from mice injected with 125I-labeled MAb demonstrated that the radioactivity was localized in the tumor tissues and not in the stromal connective tissue of mouse origin. The highest radioactivity concentration was localized in areas known to contain CEA such as the pseudolumen of glands and the apical side of carcinoma cells. The penetration of radioactivity in the central part of tumor nodules and the pseudolumen appeared to be increased with the use of MAb fragments.

Optoacoustic 3D whole-body tomography: experiments in nude mice

2009 ◽  
Author(s):  
Hans-Peter Brecht ◽  
Richard Su ◽  
Matt Fronheiser ◽  
Sergey A. Ermilov ◽  
André Conjusteau ◽  
...  
Keyword(s):  
Nude Mice ◽  
Whole Body

The Role of Whole- Body Diffusion Imaging with Background Signal Suppression (DWBS) in comparison with PET/CT in assessment of lymphoma patients

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Randa Hossein Abdallah ◽  
Samer Malak Botros ◽  
Amal Ibrahim Ahmed Othman ◽  
Mohamed Mahmoud Ibrahim Aboshanab
Keyword(s):  
Fdg Pet ◽  
Whole Body ◽  
Malignant Lymphomas ◽  
Pet Ct ◽  
Accuracy Measures ◽  
Sensitivity Specificity ◽  
Fdg Pet Ct ◽  
Post Therapy ◽  
Initial Staging ◽  
Higher Sensitivity

Abstract Background Malignant lymphomas [Hodgkin lymphomas (HL) and non-Hodgkin lymphoma (NHL)] rank third in incidence, of all childhood cancers, Moreover, in adolescents (aged 15-19 years) the malignant lymphomas are the leading cause of cancer. Furthermore, as with many other cancers, the likelihood of an individual being diagnosed with lymphoma increases markedly with age, with the median age at diagnosis being 67 years. Aim of the Work To compare between F-18-FDG PET-CT and whole-body diffusion-weighted imaging MR protocol (DWIBS) for initial staging and post chemotherapy evaluation in patients with pathologically proven lymphoma (Hodgkin and Non-Hodgkin). Patients and Methods The study is conducted on 32 patients with pathologically proven lymphoma to perform 18-F-FDG PET/CT either for pre-treatment initial staging or for evaluation of response to chemotherapy. A total of 22 had HD (69%) and 10 had NHL (31%). Staging PET/CT and WB-MRI/DWIBS were done at time of diagnosis in 19 of the 32 patients (59.4%), where immediate post-therapy PET/CT and WB-MRI-DWIBS were performed 13 patients (40.6%). Accuracy measures were calculated for PET CT and DWIBS. Results Accuracy measures confirm the higher sensitivity and specificity of PET-CT over DWIBS. Results for F-18 FDG PET/CT were clearly superior with statistically higher sensitivity, specificity, accuracy, PPV & NPV (96.3%, 99.16%, 98.43%, 97.5% and 98.75%) compared to (83.95%, 97.91%, 94.38%, 93.15% and 94.74%) for DWIBS results respectively. Conclusion F-18-FDG PET-CT remains a cornerstone in the evaluation of malignant lymphoma patients; it has significant higher sensitivity, specificity and overall accuracy compared to WB-MRI/DWIBS in HL and NHL patients, either in initial staging or in post therapy evaluation. WB-MRI/DWIBS in HL and NHL patients, either in initial staging or in post therapy evaluation. WB-MRI/DWIBS despite of its relatively longer acquisition time may provide a complementary tool for FDG PET CT.

The Effect of Whole-Body Irradiation of Nude Mice on the Tumor Transplantability and Control Probability of a Human Soft Tissue Sarcoma Xenograft

1996 ◽  
Vol 145 (3) ◽  
pp. 337 ◽  
Author(s):  
Peigen Huang ◽  
Alphonse Taghian ◽  
Ayman Allam ◽  
Jill Freeman ◽  
Michael Duffy ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Nude Mice ◽  
Whole Body ◽  
Human Soft Tissue ◽  
And Control
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