Subwavelength-resolution fluorescent correlation spectroscopy using a solid immersion lens

2004 ◽  
Author(s):  
Alexandre Serov ◽  
Ramachandra G. Rao ◽  
Michael Gosch ◽  
Tiemo Anhut ◽  
Dietrich Martin ◽  
...  
Keyword(s):  
Correlation Spectroscopy ◽  
Solid Immersion Lens ◽  
Fluorescent Correlation Spectroscopy ◽  
Subwavelength Resolution

High light field confinement for fluorescent correlation spectroscopy using a solid immersion lens

2004 ◽  
Vol 20 (3) ◽  
pp. 431-435 ◽  
Author(s):  
A. Serov ◽  
R. Rao ◽  
M. Gösch ◽  
T. Anhut ◽  
D. Martin ◽  
...  
Keyword(s):  
Light Field ◽  
High Light ◽  
Correlation Spectroscopy ◽  
Solid Immersion Lens ◽  
Fluorescent Correlation Spectroscopy

scholarly journals Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids

2014 ◽  
Vol 5 (10) ◽  
pp. 3730 ◽  
Author(s):  
Charles-Edouard Leroux ◽  
Sylvain Monnier ◽  
Irène Wang ◽  
Giovanni Cappello ◽  
Antoine Delon
Keyword(s):  
Adaptive Optics ◽  
Intercellular Space ◽  
Correlation Spectroscopy ◽  
Fluorescent Correlation Spectroscopy

Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy

2013 ◽  
Vol 455 (3) ◽  
pp. 339-345 ◽  
Author(s):  
Takayasu Mori ◽  
Eriko Kikuchi ◽  
Yuko Watanabe ◽  
Shinya Fujii ◽  
Mari Ishigami-Yuasa ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Library Screening ◽  
Chemical Library ◽  
Fluorescence Correlation ◽  
Fluorescent Correlation Spectroscopy

To discover WNK–OSR1/SPAK signalling inhibitors, we generated a new high-throughput system using fluorescent correlation spectroscopy capable of screening compounds that disrupt the binding of two molecules. We finally identified two novel and promising compounds for WNK–OSR1/SPAK signalling inhibition.

scholarly journals Fluorescent Correlation Spectroscopy Measurement in Microfluidic Device

2020 ◽  
Vol 118 (3) ◽  
pp. 617a
Author(s):  
Dayo D. Adeyemo ◽  
Praise Farayola ◽  
Oluwaseun Egunsola ◽  
Bernard M. Hangòrmbe
Keyword(s):  
Microfluidic Device ◽  
Correlation Spectroscopy ◽  
Spectroscopy Measurement ◽  
Fluorescent Correlation Spectroscopy

scholarly journals Spying on IgE receptor signaling

2005 ◽  
Vol 171 (3) ◽  
pp. 415-417
Author(s):  
Derek Toomre
Keyword(s):  
Signal Transduction ◽  
Protein Interactions ◽  
Lipid Raft ◽  
Correlation Spectroscopy ◽  
Receptor Signaling ◽  
Protein Protein Interactions ◽  
Ige Receptor ◽  
Fluorescent Correlation Spectroscopy ◽  
Controversial Topic ◽  
Lipid Raft Microdomains

Plasma membrane organization and the potential role, or not, of lipid raft microdomains in signal transduction is a controversial topic. Cross-correlation fluorescent correlation spectroscopy (CC-FCS) shows promise as a new approach to rapidly probe protein–protein interactions in living cells during signal transduction. CC-FCS data from studies of IgE receptor signaling challenge models of large stable lipid raft signaling domains and reveal a new complexity in the dynamic (re)organization of signaling complexes.

scholarly journals Analysis of Interactions of Signaling Proteins with Phage-Displayed Ligands by Fluorescence Correlation Spectroscopy

2008 ◽  
Vol 13 (8) ◽  
pp. 766-776
Author(s):  
Helmi R.M. Schlaman ◽  
Kristiane Schmidt ◽  
Dorien Ottenhof ◽  
Maarten H. van Es ◽  
Tjerk H. Oosterkamp ◽  
...  
Keyword(s):  
Good Alternative ◽  
Correlation Spectroscopy ◽  
Peptide Ligands ◽  
Peptide Ligand ◽  
Binding Affinities ◽  
Phage Display Technology ◽  
Force Microscopy ◽  
Fluorescent Correlation Spectroscopy ◽  
Atomic Force ◽  
Display Technology

Fluorescent correlation spectroscopy (FCS) was used to measure binding affinities of ligands to ligates that are expressed by phage-display technology. Using this method we have quantified the binding of the 14-3-3 signaling protein to artificial peptide ligand. As a ligand we used the R18 artificial peptide expressed as a fusion in the cpIII coat protein that is present in 3 to 5 copies in an M13 phage. Comparisons of binding affinities were made with free R18 ligands using FCS. The result showed a relatively high binding affinity for the phage-displayed R18 peptide compared with binding to free fluorescently labeled R18. Quantification was supported by titration of the phage numbers using atomic force microscopy (AFM). AFM was shown to accurately determine phage numbers in solution as a good alternative for electron microscopy. It was shown to give reliable data that correlated perfectly with those of the viable phage numbers determined by classical bacterial infection studies. In conclusion, a very fast and sensitive method for the selection of new peptide ligands or ligates based on a quantitative assay in solution has been developed. ( Journal of Biomolecular Screening 2008:766-776)

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