Estimation of imaging performance using local optical quality factor metrics

1992 ◽  
Vol 31 (5) ◽  
pp. 1038 ◽  
Author(s):  
Jerold B. Lisson
Vibration ◽  
2018 ◽  
Vol 1 (2) ◽  
pp. 239-249 ◽  
Author(s):  
Edoardo Rubino ◽  
Tindaro Ioppolo

This paper addresses the feasibility of an optical vibrometer that is based on the shift of the optical modes, also known as whispering gallery modes (WGMs), of a magnetorheological optical resonator. The optical resonator that is used in this study is fabricated by mixing polyvinyl chloride plastisol with magnetically polarizable particles. When a permanent magnet that is located nearby the optical resonator is moved, it induces a perturbation of the morphology of the resonator, due to the magnetostrictive effect. This change in the morphology induces a shift in the optical modes of the resonator. The shift of the optical modes can be related to the displacement of the permanent magnet. The proposed sensor concept is based on monitoring the displacement of a tiny magnet that is attached to a moving surface. The optical quality factor of the resonator used in these studies was of the order of 106. The experimental results show a sensitivity of 0.32 pm/μm and a resolution that is less than 300 nm.


2019 ◽  
Author(s):  
Katharina Hötte ◽  
Michael Koch ◽  
Lotta Hof ◽  
Marcel Tuppi ◽  
Till Moreth ◽  
...  

AbstractIn three-dimensional light microscopy, the heterogeneity of the optical density in a specimen ultimately limits the achievable penetration depth and hence the three-dimensional resolution. The most direct approach to reduce aberrations, improve the contrast, and achieve an optimal resolution is minimizing the impact of changes of the refractive index along an optical path. Many light sheet fluorescence microscopes operate with a large chamber that contains an aqueous immersion medium and an inner specimen holder that contains the specimen embedded in a possibly entirely different non-aqueous medium. In order to minimize the impact of the specimen holder on the optical quality, we use multi-facetted cuvettes fabricated with vacuum-formed ultra-thin fluorocarbon (FEP) foils The ultra-thin FEP-foil cuvettes have a wall thickness of about 12 µm. They are resilient to fluidic exchanges, durable, mechanically stable and yet flexible.We confirm the improved imaging performance of ultra-thin FEP-foil cuvettes with excellent quality images of whole organs, thick tissue sections and dense organoid clusters. The cuvettes outperform many other sample-mounting techniques in terms of full separation of the specimen from the immersion medium, compatibility with aqueous and organic clearing media, quick specimen mounting without hydrogel embedding, as well as their applicability for multiple-view imaging and automated segmentation. Additionally, we show that ultra-thin FEP foil cuvettes are suitable for seeding and growing organoids over a time period of at least ten days. The ultra-thin cuvettes allow the fixation and staining of the specimens inside the holder, preserving the delicate morphology of e.g. fragile, mono-layered three-dimensional organoids.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Katharina Hötte ◽  
Michael Koch ◽  
Lotta Hof ◽  
Marcel Tuppi ◽  
Till Moreth ◽  
...  

AbstractIn three-dimensional light microscopy, the heterogeneity of the optical density in a specimen ultimately limits the achievable penetration depth and hence the three-dimensional resolution. The most direct approach to reduce aberrations, improve the contrast and achieve an optimal resolution is to minimise the impact of changes of the refractive index along an optical path. Many implementations of light sheet fluorescence microscopy operate with a large chamber filled with an aqueous immersion medium and a further inner container with the specimen embedded in a possibly entirely different non-aqueous medium. In order to minimise the impact of the latter on the optical quality of the images, we use multi-facetted cuvettes fabricated from vacuum-formed ultra-thin fluorocarbon (FEP) foils. The ultra-thin FEP-foil cuvettes have a wall thickness of about 10–12 µm. They are impermeable to liquids, but not to gases, inert, durable, mechanically stable and flexible. Importantly, the usually fragile specimen can remain in the same cuvette from seeding to fixation, clearing and observation, without the need to remove or remount it during any of these steps. We confirm the improved imaging performance of ultra-thin FEP-foil cuvettes with excellent quality images of whole organs such us mouse oocytes, of thick tissue sections from mouse brain and kidney as well as of dense pancreas and liver organoid clusters. Our ultra-thin FEP-foil cuvettes outperform many other sample-mounting techniques in terms of a full separation of the specimen from the immersion medium, compatibility with aqueous and organic clearing media, quick specimen mounting without hydrogel embedding and their applicability for multiple-view imaging and automated image segmentation. Additionally, we show that ultra-thin FEP foil cuvettes are suitable for seeding and growing organoids over a time period of at least ten days. The new cuvettes allow the fixation and staining of specimens inside the holder, preserving the delicate morphology of e.g. fragile, mono-layered three-dimensional organoids.


2015 ◽  
Vol 27 (20) ◽  
pp. 2197-2200 ◽  
Author(s):  
Patrick Steglich ◽  
Christian Mai ◽  
David Stolarek ◽  
Stefan Lischke ◽  
Sebastian Kupijai ◽  
...  

2018 ◽  
Vol 185 ◽  
pp. 02016 ◽  
Author(s):  
Tatyana Mikhailova ◽  
Alexander Shaposhnikov ◽  
Anatoly Prokopov ◽  
Andrey Karavainikov ◽  
Sergey Tomilin ◽  
...  

New original Tamm plasmon-polaritons (TPP) structures with Bi-substituted iron garnet and Au plasmonic layers were proposed, synthesized and investigated. The structures with single and double garnet layers were modelled to form a TPP mode at the center of photonic band gab. The top Au layer has the gradient thickness varied in the range from 0 to 65.2 nm. It was found the features of TPP resonances as a function of the thickness of metal coating. The resonances on TPP have the maximum optical quality factor and transmission at the vicinity of Au thickness of 30 nm. These configurations are optimum to form the highest intensity of electric field of light wave in the area of the magnetic layers. It was found the spectral blue and red shifts of TPP mode with increasing of Au thickness. The blue and red shifts can be explained respectively by structural and thickness changes of Au coating. The maximum resonant values of Faraday rotation were –2.1° at 664 nm and –12.3° at 645 nm for structures with single and double garnet layers, respectively, and thickness of Au coating of 65.2 nm.


Author(s):  
Sasikanth Manipatruni ◽  
Carl Poitras ◽  
Qianfan Xu ◽  
Michal Lipson

2008 ◽  
Vol 33 (15) ◽  
pp. 1644 ◽  
Author(s):  
Sasikanth Manipatruni ◽  
Carl B. Poitras ◽  
Qianfan Xu ◽  
Michal Lipson

Sign in / Sign up

Export Citation Format

Share Document