Kinetics of camptothecin hydrolysis determined by PCA and FA analysis of fluorescence spectra

2003 ◽  
Author(s):  
Stefan Kruszewski ◽  
Ryszard Siuda ◽  
Blanka Ziomkowska ◽  
Michal Cyrankiewicz
Keyword(s):  
Fluorescence Spectra ◽  
Kinetics Of

Fluorescence spectra and kinetics of isomers and dimers of retinoic acid

1990 ◽  
Vol 53 (6) ◽  
pp. 1271-1275 ◽  
Author(s):  
M. V. Bel'kov ◽  
S. L. Bondarev
Keyword(s):  
Retinoic Acid ◽  
Fluorescence Spectra ◽  
Kinetics Of

Photochromism, Kinetics and Fluorescence of 1-(2-methyl-3-benzofuranyl)-2-[2-methyl-5-(4-cyanophenyl)-3-thienyl]perfluorocyclopentene

2014 ◽  
Vol 886 ◽  
pp. 175-178
Author(s):  
Dan Dan Xue ◽  
Zhi Yuan Sun ◽  
Shou Zhi Pu
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Photochromic Properties ◽  
Strong Fluorescence ◽  
Open Ring ◽  
Kinetics Of ◽  
Hexane Solution

An unsymmetrical photochromic diarylethene compound, 1-(2-methyl-3-benzofuranyl)-2-[2-methyl-5-(4-cyanophenyl)-3-thienyperfluorocyclopentene was synthesized, and its photochromic properties, kinetics of the photochromic cyclization/cycloreversion and fluorescence were investigated in detail. The compound exhibited good photochromism, changing from colorless to red after irradiation with 297 nm UV light, in which absorption maxima were observed at 530 nm in hexane solution. Simultaneously, cyclization/cycloreversion kinetics of this diarylethene was researched. The open-ring isomer of the diarylethene exhibited relatively strong fluorescence at 377 nm in hexane solution (2 × 10-5 mol L-1), when excited at 327 nm. The fluorescence intensity decreased along with the photochromism upon irradiation with 297 nm light. The fluorescence spectra of the diarylethene are depended on the concentration.

Inactivation and conformational changes of creatine kinase at low concentrations of hexafluoroisopropanol solutions

2003 ◽  
Vol 81 (5) ◽  
pp. 327-333 ◽  
Author(s):  
Xiao-Yun Wang ◽  
Fan-Guo Meng ◽  
Hai-Meng Zhou
Keyword(s):  
Creatine Kinase ◽  
Enzyme Activity ◽  
Conformational Changes ◽  
Fluorescence Spectra ◽  
Cd Spectra ◽  
Irreversible Inhibition ◽  
Low Concentrations ◽  
Flexible Region ◽  
Far Ultraviolet ◽  
Kinetics Of

Using the methods of far-ultraviolet circular dichroism (CD) spectra, fluorescence spectra, and enzyme activity assays, the inactivation and conformational changes of creatine kinase (CK) induced by 1,1,1,3,3,3-hexafluoro-2-propanol (hexafluoroisopropanol (HFIP)) of different concentrations were investigated. To avoid the aggregation of CK that occurs with high HFIP, concentrations of 0%–5% HFIP were used in this study. The CD spectra showed that HFIP concentrations above 2.5% strongly induced the formation of secondary structures of CK. No marked conformational changes were observed at low concentrations of HFIP (0%–2.5%). After incubation with 0.2% HFIP for 10 min, CK lost most of its activity. The kinetic theory of the substrate reaction during irreversible inhibition of enzyme activity described previously by Tsou was applied to study the kinetics of CK inactivation during denaturation by HFIP. The inactivation rate constants for the free enzyme and the substrate–enzyme complex were determined by Tsou's method. The results suggested that low concentrations of HFIP had a high potential to induce helices of protein and that the active site of the enzyme was situated in a limited and flexible region of the enzyme molecule that was more susceptible to the denaturant than was the protein as a whole.Key words: creatine kinase, inactivation, conformation, kinetics, hexafluoroisopropanol.

Fluorescence spectra and kinetics of Cs2

1979 ◽  
Vol 70 (7) ◽  
pp. 3155-3164 ◽  
Author(s):  
R. P. Benedict ◽  
D. L. Drummond ◽  
L. A. Schlie
Keyword(s):  
Fluorescence Spectra ◽  
Kinetics Of

Fluorescence Reagents for Labelling of Biomolecules. Part III. Study of the Reactions of 2- and 4-Substituted 9-Isothiocyanatoacridines with Glycine

1994 ◽  
Vol 59 (7) ◽  
pp. 1682-1690 ◽  
Author(s):  
Danica Sabolová ◽  
Dana Mazagová ◽  
Pavol Kristian ◽  
Marián Antalík ◽  
Dušan Podhradský ◽  
...  
Keyword(s):  
Reaction Mechanism ◽  
Fluorescence Intensity ◽  
Nucleophilic Addition ◽  
Fluorescence Spectra ◽  
Addition Reaction ◽  
Kinetic Characteristics ◽  
Reaction Products ◽  
H Nmr ◽  
Nucleophilic Addition Reaction ◽  
Kinetics Of

Kinetics of nucleophilic addition reaction of 2- and 4-substituted 9-isothiocyanatoacridines I - VII with glycine in buffered aqueous dimethylformamide has been studied. The addition products, N-(9-acridinylthiocarbamoyl)glycines VIII - XIV, were characterized by IR, UV, 1H NMR, mass and fluorescence spectra. Derivatives VIII, X and XII exhibited higher fluorescence intensity than the starting isothiocyanates; the highest fluorescence was found for the unsubstituted compound X. The reaction mechanism is discussed on the basis of properties of the reaction products and kinetic characteristics.

Fluorescence spectra and decay kinetics of 1,1'-binaphthyl and related compounds at high pressures

1978 ◽  
Vol 47 ◽  
pp. 371-378 ◽  
Author(s):  
Malcolm F. Nicol ◽  
Yoji Hara ◽  
Joseph M. Wiget ◽  
Mary Anton
Keyword(s):  
Fluorescence Spectra ◽  
High Pressures ◽  
Decay Kinetics ◽  
Kinetics Of ◽  
Related Compounds

Photochromic, Kinetics, and Fluorescence of a Novel Diarylethene

2012 ◽  
Vol 490-495 ◽  
pp. 3301-3305
Author(s):  
Xiao Ting Li ◽  
Shou Zhi Pu ◽  
Hui Li
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Amorphous Film ◽  
Photochromic Properties ◽  
Strong Fluorescence ◽  
First Order ◽  
Closed Ring ◽  
Open Ring ◽  
Kinetics Of ◽  
Hexane Solution

A new unsymmetrical photochromic diarylethene, 1-(2-ethyl-3-benzofuranyl)-2-(2,5 -dimethyl-3-thienyl)perfluorocyclopentene (1) was synthesized, and its photochromic properties and kinetics of the photochromic cyclization/cycloreversion were investigated. The results showed that the cyclization/cycloreversion process of these compounds were determined to be the zeroth/first order reaction.The compound exhibited good photochromism in hexane solution with alternating irradiation by UV/Vis light, and the maxima absorption of its closed-ring isomer 1c are 502 nm. The open-ring isomer of the diarylethene 1 exhibited relatively strong fluorescence at 402 nm in hexane solution (2 × 10-5 mol/L) and 412 nm in PMMA amorphous film when excited at 312 nm and 310 nm,respectively. The fluorescence intensity decreased along with the photochromism upon irradiation with 297 nm light and its closed-ring isomer showed almost no fluorescence.The fluorescence spectra of diarylethene 1o depended on the concentration.

ChemInform Abstract: FLUORESCENCE SPECTRA AND KINETICS OF CESIUM DIMER

1979 ◽  
Vol 10 (34) ◽  
Author(s):  
R. P. BENEDICT ◽  
D. L. DRUMMOND ◽  
L. A. SCHLIE
Keyword(s):  
Fluorescence Spectra ◽  
Kinetics Of

Unusual Temperature Dependence of the Fluorescence Decay in Heterostructured Stilbene

2021 ◽  
Author(s):  
Renata Karpicz ◽  
Nina Ostapenko ◽  
Yura Ostapenko ◽  
Yaroslava Polupan ◽  
Igor Lazarev ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Wide Temperature Range ◽  
Fluorescence Spectra ◽  
Room Temperature ◽  
Fluorescence Decay ◽  
Decay Kinetics ◽  
Temperature Range ◽  
Fluorescence Decay Kinetics ◽  
Kinetics Of

Fluorescence spectra as well as fluorescence decay kinetics of hot-pressed and sublimated films of stilbene has been studied in wide temperature range, from 15 K up to room temperature. Fluorescence...

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