Third-generation GFP biosensors for real-time readout of pH and redox potential in living cells

2004 ◽  
Author(s):  
Stephen J. Remington ◽  
George T. Hanson ◽  
Mark Canon ◽  
Robert Aggeler ◽  
Devin Oglesbe ◽  
...  
Keyword(s):  
Redox Potential ◽  
Real Time ◽  
Living Cells ◽  
Third Generation ◽  
Time Readout

scholarly journals Real-time observation of light-controlled transcription in living cells

2017 ◽  
Author(s):  
Anne Rademacher ◽  
Fabian Erdel ◽  
Jorge Trojanowski ◽  
Karsten Rippe
Keyword(s):  
Real Time ◽  
Gene Cluster ◽  
Living Cells ◽  
Effector Proteins ◽  
High Temporal Resolution ◽  
Activation Process ◽  
Osteosarcoma Cell ◽  
Dynamic Regulation ◽  
Time Observation ◽  
Time Readout

AbstractGene expression is a tightly controlled process that is coordinated in space and time. To dissect its dynamic regulation with high temporal resolution, we introduce an optogenetic tool termed BLInCR (Blue Light-Induced Chromatin Recruitment) that combines rapid and reversible light-dependent recruitment of effector proteins with a real-time readout for transcription. We used BLInCR to control the activity of a reporter gene cluster in the human osteosarcoma cell line U2OS by reversibly recruiting the viral transactivator VP16. RNA production was detectable ∼2 minutes after VP16 recruitment and readily decreased when VP16 dissociated from the cluster in the absence of light. Quantitative assessment of the activation process revealed biphasic activation kinetics with a pronounced early phase in cells treated with the histone deacetylase inhibitor SAHA. Comparison with kinetic models for transcription activation suggests that the gene cluster undergoes a maturation process when activated. BLInCR will facilitate the study of transcription dynamics in living cells.

A multicolor DNA tetrahedron nanoprobe for analyzing human telomerase in living cells

2021 ◽  
Author(s):  
Ruiyuan Zhang ◽  
Ruixue Zhang ◽  
Wei Jiang ◽  
Xiaowen Xu
Keyword(s):  
Real Time ◽  
Length Distribution ◽  
Telomerase Activity ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Dna Tetrahedron ◽  
Human Telomerase

A sequentially lighting-up multicolor DNA tetrahedron nanoprobe is constructed for imaging telomerase activity, real-time monitoring telomerase action and determining product length distribution in living cells.

Highly sensitive real-time detection of intracellular oxidative stress and application in mycotoxin toxicity evaluation based on living single-cell electrochemical sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Lu Gao ◽  
Jiadi Sun ◽  
Liping Wang ◽  
Qigao Fan ◽  
Gaowen Zhu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electrochemical Sensor ◽  
Single Cell ◽  
Real Time ◽  
Electrochemical Sensors ◽  
Living Cells ◽  
Selective Detection ◽  
Toxicity Evaluation ◽  
Highly Sensitive ◽  
Intracellular Oxidative Stress

Single-cell electrochemical sensor is used in the local selective detection of living cells because of its high spatial–temporal resolution and sensitivity, as well as its ability to obtain comprehensive cellular physiological states and processes.

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