Three-dimensional in vivo imaging by spectral OCT

2004 ◽  
Author(s):  
Tomasz Bajraszewski ◽  
Maciej Wojtkowski ◽  
Piotr Targowski ◽  
Maciej Szkulmowski ◽  
Andrzej Kowalczyk
Keyword(s):  
In Vivo Imaging ◽  
Three Dimensional ◽  
Spectral Oct

scholarly journals High-resolution three-dimensional in vivo imaging of atherosclerotic plaque

1999 ◽  
Vol 42 (4) ◽  
pp. 762-771 ◽  
Author(s):  
Gerard T. Luk-Pat ◽  
Garry E. Gold ◽  
Eric W. Olcott ◽  
Bob S. Hu ◽  
Dwight G. Nishimura
Keyword(s):  
High Resolution ◽  
Atherosclerotic Plaque ◽  
In Vivo Imaging ◽  
Three Dimensional

Three-Dimensional In Vivo Imaging of the Mouse Intraocular Vasculature during Development and Disease

2005 ◽  
Vol 46 (9) ◽  
pp. 3021 ◽  
Author(s):  
Matthew R. Ritter ◽  
Edith Aguilar ◽  
Eyal Banin ◽  
Lea Scheppke ◽  
Hannele Uusitalo-Jarvinen ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Three Dimensional

scholarly journals Optimized murine lung preparation for detailed structural evaluation via micro-computed tomography

2012 ◽  
Vol 112 (1) ◽  
pp. 159-166 ◽  
Author(s):  
Dragoş M. Vasilescu ◽  
Lars Knudsen ◽  
Matthias Ochs ◽  
Ewald R. Weibel ◽  
Eric A. Hoffman
Keyword(s):  
In Vivo Imaging ◽  
Airway Pressure ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Imaging Method ◽  
Small Airways ◽  
Micro Computed Tomography ◽  
Vascular Perfusion ◽  
Murine Lung

Utilizing micro-X-ray CT (μCT) imaging, we sought to generate an atlas of in vivo and intact/ex vivo lungs from normal murine strains. In vivo imaging allows visualization of parenchymal density and small airways (15–28 μm/voxel). Ex vivo imaging of the intact lung via μCT allows for improved understanding of the three-dimensional lung architecture at the alveolar level with voxel dimensions of 1–2 μm. μCT requires that air spaces remain air-filled to detect alveolar architecture while in vivo structural geometry of the lungs is maintained. To achieve these requirements, a fixation and imaging methodology that permits nondestructive whole lung ex vivo μCT imaging has been implemented and tested. After in vivo imaging, lungs from supine anesthetized C57Bl/6 mice, at 15, 20, and 25 cmH2O airway pressure, were fixed in situ via vascular perfusion using a two-stage flushing system while held at 20 cmH2O airway pressure. Extracted fixed lungs were air-dried. Whole lung volume was acquired at 1, 7, 21, and >70 days after the lungs were dried and served as validation for fixation stability. No significant shrinkage was observed: +8.95% change from in vivo to fixed lung ( P = 0.12), −1.47% change from day 1 to day 7 ( P = 0.07), −2.51% change from day 1 to day 21 ( P = 0.05), and −4.90% change from day 1 to day 70 and thereafter ( P = 0.04). μCT evaluation showed well-fixed alveoli and capillary beds correlating with histological analysis. A fixation and imaging method has been established for μCT imaging of the murine lung that allows for ex vivo morphometric analysis, representative of the in vivo lung.

Three-Dimensional In Vivo Imaging of Tumors Expressing Red Fluorescent Proteins

2012 ◽  
pp. 97-114 ◽  
Author(s):  
Alexander P. Savitsky ◽  
Irina G. Meerovich ◽  
Victoria V. Zherdeva ◽  
Lyaysan R. Arslanbaeva ◽  
Olga S. Burova ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Proteins ◽  
Three Dimensional ◽  
Red Fluorescent Proteins

scholarly journals Three-Dimensional in Vivo Imaging of Green Fluorescent Protein-Expressing T Cells in Mice with Noncontact Fluorescence Molecular Tomography

2007 ◽  
Vol 6 (2) ◽  
pp. 7290.2007.00007 ◽  
Author(s):  
Anikitos Garofalakis ◽  
Giannis Zacharakis ◽  
Heiko Meyer ◽  
Eleftherios N. Economou ◽  
Clio Mamalaki ◽  
...  
Keyword(s):  
T Cells ◽  
Green Fluorescent Protein ◽  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Three Dimensional ◽  
Fluorescence Molecular Tomography ◽  
Green Fluorescent

Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes

2012 ◽  
Vol 9 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Gergely Katona ◽  
Gergely Szalay ◽  
Pál Maák ◽  
Attila Kaszás ◽  
Máté Veress ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Three Dimensional ◽  
Random Access ◽  
Two Photon

scholarly journals Three-Dimensional In Vivo Imaging of the Murine Liver: A Micro-Computed Tomography-Based Anatomical Study

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31179 ◽  
Author(s):  
Teresa Fiebig ◽  
Hanne Boll ◽  
Giovanna Figueiredo ◽  
Hans Ulrich Kerl ◽  
Stefanie Nittka ◽  
...  
Keyword(s):  
Computed Tomography ◽  
In Vivo Imaging ◽  
Three Dimensional ◽  
Anatomical Study ◽  
Micro Computed Tomography ◽  
Murine Liver

scholarly journals Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope

2008 ◽  
Vol 16 (10) ◽  
pp. 7224 ◽  
Author(s):  
Hyejun Ra ◽  
Wibool Piyawattanametha ◽  
Michael J. Mandella ◽  
Pei-Lin Hsiung ◽  
Jonathan Hardy ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Three Dimensional ◽  
Confocal Microscope
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