Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes

2012 ◽  
Vol 9 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Gergely Katona ◽  
Gergely Szalay ◽  
Pál Maák ◽  
Attila Kaszás ◽  
Máté Veress ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Three Dimensional ◽  
Random Access ◽  
Two Photon

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

Three-dimensional in vivo imaging by spectral OCT

2004 ◽  
Author(s):  
Tomasz Bajraszewski ◽  
Maciej Wojtkowski ◽  
Piotr Targowski ◽  
Maciej Szkulmowski ◽  
Andrzej Kowalczyk
Keyword(s):  
In Vivo Imaging ◽  
Three Dimensional ◽  
Spectral Oct

scholarly journals Two-Photon Time-Gated In Vivo Imaging of Dihydrolipoic-Acid-Decorated Gold Nanoclusters

Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7744
Author(s):  
Ye Tian ◽  
Ming Wei ◽  
Lijun Wang ◽  
Yuankai Hong ◽  
Dan Luo ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Gold Nanoclusters ◽  
Photon Absorption ◽  
Gold Nanocluster ◽  
Time Resolved ◽  
Dihydrolipoic Acid ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Time Resolved Imaging

Due to the unique advantages of two-photon technology and time-resolved imaging technology in the biomedical field, attention has been paid to them. Gold clusters possess excellent physicochemical properties and low biotoxicity, which make them greatly advantageous in biological imaging, especially for in vivo animal imaging. A gold nanocluster was coupled with dihydrolipoic acid to obtain a functionalized nanoprobe; the material displayed significant features, including a large two-photon absorption cross-section (up to 1.59 × 105 GM) and prolonged fluorescence lifetime (>300 ns). The two-photon and time-resolution techniques were used to perform cell imaging and in vivo imaging.

A bright blue fluorescent dextran for two-photon in vivo imaging of blood vessels

2019 ◽  
Vol 89 ◽  
pp. 103019 ◽  
Author(s):  
Seo Hyeon Lee ◽  
Young Ho Choe ◽  
Rae Hyung Kang ◽  
Yu Rim Kim ◽  
Na Hee Kim ◽  
...  
Keyword(s):  
Blood Vessels ◽  
In Vivo Imaging ◽  
Two Photon ◽  
Fluorescent Dextran ◽  
Bright Blue

scholarly journals In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 3303 ◽  
Author(s):  
Seoyeon Bok ◽  
Taejun Wang ◽  
Chan-Ju Lee ◽  
Seong-Uk Jeon ◽  
Young-Eun Kim ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Mouse Model ◽  
In Vivo Imaging ◽  
Focal Cerebral Ischemia ◽  
Activated Microglia ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals High-resolution three-dimensional in vivo imaging of atherosclerotic plaque

1999 ◽  
Vol 42 (4) ◽  
pp. 762-771 ◽  
Author(s):  
Gerard T. Luk-Pat ◽  
Garry E. Gold ◽  
Eric W. Olcott ◽  
Bob S. Hu ◽  
Dwight G. Nishimura
Keyword(s):  
High Resolution ◽  
Atherosclerotic Plaque ◽  
In Vivo Imaging ◽  
Three Dimensional

Design of molecular two-photon probes for in vivo imaging. 2H-Benzo[h]chromene-2-one derivatives

2007 ◽  
Vol 48 (15) ◽  
pp. 2791-2795 ◽  
Author(s):  
Hwan Myung Kim ◽  
Xing Zhong Fang ◽  
Pil Rye Yang ◽  
Jae-Sung Yi ◽  
Young-Gyu Ko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Two Photon
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