Investigation of doxorubicin for multidrug resistance using a fluorescent cytometric imaging system integrated onto cell culture analog devices

2004 ◽  
Author(s):  
Donghyun Kim ◽  
Hui Xu ◽  
Sung J. Kim ◽  
Michael L. Shuler
Keyword(s):  
Cell Culture ◽  
Multidrug Resistance ◽  
Imaging System

scholarly journals Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy

2016 ◽  
Vol 7 (8) ◽  
pp. 3097 ◽  
Author(s):  
Jinho Kim ◽  
Beverley M. Henley ◽  
Charlene H. Kim ◽  
Henry A. Lester ◽  
Changhuei Yang
Keyword(s):  
Cell Culture ◽  
Imaging System

scholarly journals Interstitial Flow Recapitulates Gemcitabine Chemoresistance in A 3D Microfluidic Pancreatic Ductal Adenocarcinoma Model by Induction of Multidrug Resistance Proteins

2019 ◽  
Vol 20 (18) ◽  
pp. 4647 ◽  
Author(s):  
Bart Kramer ◽  
Luuk de Haan ◽  
Marjolein Vermeer ◽  
Thomas Olivier ◽  
Thomas Hankemeier ◽  
...  
Keyword(s):  
Cell Culture ◽  
Multidrug Resistance ◽  
Pancreatic Ductal Adenocarcinoma ◽  
3D Cell Culture ◽  
In Vitro Models ◽  
Ductal Adenocarcinoma ◽  
Interstitial Flow ◽  
Multidrug Resistance Proteins ◽  
And Function

Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most lethal cancers due to a high chemoresistance and poor vascularization, which results in an ineffective systemic therapy. PDAC is characterized by a high intratumoral pressure, which is not captured by current 2D and 3D in vitro models. Here, we demonstrated a 3D microfluidic interstitial flow model to mimic the intratumoral pressure in PDAC. We found that subjecting the S2-028 PDAC cell line to interstitial flow inhibits the proliferation, while maintaining a high viability. We observed increased gemcitabine chemoresistance, with an almost nine-fold higher EC50 as compared to a monolayer culture (31 nM versus 277 nM), and an alleviated expression and function of the multidrug resistance protein (MRP) family. In conclusion, we developed a 3D cell culture modality for studying intratissue pressure and flow that exhibits more predictive capabilities than conventional 2D cell culture and is less time-consuming, and more scalable and accessible than animal models. This increase in microphysiological relevance might support improved efficiency in the drug development pipeline.

scholarly journals An embedded application for cell culture confluency estimation using the InCellis® Smart Cell Imaging System

BioTechniques ◽  
2018 ◽  
Vol 64 (3) ◽  
pp. 127
Author(s):  
Laima Antanaviciute ◽  
Sophie Dubacq ◽  
Olivier Varet
Keyword(s):  
Cell Culture ◽  
Cell Imaging ◽  
Imaging System ◽  
Embedded Application

scholarly journals Incubator Embedded Cell Culture Imaging System (EmSight) Based on Fourier Ptychographic Microscopy

2017 ◽  
Author(s):  
Jinho Kim ◽  
Beverley M. Henley ◽  
Charlene H. Kim ◽  
Henry A. Lester ◽  
Changhuei Yang
Keyword(s):  
Cell Culture ◽  
Imaging System

scholarly journals Removal of digoxin and doxorubicin by multidrug resistance protein-overexpressed cell culture in hollow fiber

1999 ◽  
Vol 56 (1) ◽  
pp. 154-163 ◽  
Author(s):  
Shuichi Tsuruoka ◽  
Koh-Ichi Sugimoto ◽  
Kazumitsu Ueda ◽  
Makoto Suzuki ◽  
Masashi Imai ◽  
...  
Keyword(s):  
Cell Culture ◽  
Multidrug Resistance ◽  
Hollow Fiber ◽  
Multidrug Resistance Protein ◽  
Resistance Protein

scholarly journals A portable cell culture system with a simple microscope, oxygen sensor and MEA amplifier

2019 ◽  
Author(s):  
Dhanesh Kattipparambil Rajan
Keyword(s):  
Cell Culture ◽  
Chemical Sensing ◽  
Live Cell Imaging ◽  
Oxygen Sensor ◽  
Cell Imaging ◽  
Imaging System ◽  
Electrophysiological Recording

Cell culture in-vitro is a well-known method to develop cell and disease models for studying physiologically relevant mechanisms and responses for various applications in life sciences. Conventional methods for instance, using static culture flasks or well plates, have limitations, as these cannot provide accurate tractable models for advanced studies. However, microscale systems can overcome this since they mimic the cells' natural microenvironment adequately. We have developed a portable live-cell imaging system with an invert-upright-convertible architecture and a mini-bioreactor for long-term simultaneous cell imaging and analysis, chemical sensing and electrophysiological recording. Our system integrates biocompatible cell-friendly materials with modular measurement schemes and precise environment control and can be automated. High quality time-lapse cell imaging is hugely useful in cell/disease models. However, integration of advanced in-vitro systems into benchtop microscopes for in-situ imaging is tricky and challenging. This is especially true with device based biological systems, such as lab/organ/body-on-chips, or mini-bioreactors/microfluidic systems. They face issues ranging from optical and physical geometry incompatibilities to difficulties in connectivity of flow and perfusion systems. However, the novel modular system we have developed either as an inverted or as an upright system can easily accommodate virtually any in-vitro devices. Furthermore, it can accept additional sensor or measurement devices quite freely. Cell characterization, differentiation, chemical sensing, drug screening, microelectrode-array-electrophysiological recordings, and cell stimulation can be carried out with simultaneous in-situ imaging and analysis. Moreover, our system can be configured to capture images from regions that are otherwise inaccessible by conventional microscopes, for example, cells cultured on physical or biochemical sensor systems. We demonstrate the system for video-based beating analysis of cardiomyocytes, cell orientation analysis on nanocellulose, and simultaneous long-term in-situ microscopy with pO2 and temperature sensing. The compact microscope as such is comparable to standard phase-contrast-microscopes without any detectable aberrations and is useful practically for any in-situ microscopy demands. [1] D. K. Rajan et al., "A Portable Live-Cell Imaging System With an Invert-Upright-Convertible Architecture and a Mini-Bioreactorfor Long-Term Simultaneous Cell Imaging, Chemical Sensing, and Electrophysiological Recording," in IEEE Access, vol. 6,pp. 11063-11075, 2018. doi: 10.1109/ACCESS.2018.2804378. [2] Antti-Juhana Mäki et al., "A Portable Microscale Cell Culture System with Indirect Temperature Control"SLAS TECHNOLOGY: Translating Life Sciences Innovation 2018 23:6, 566-579. [3] Hannu Välimäki et al.,Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cellmodels,Sensors and Actuators B: Chemical,Volume 249,2017,pp.738-746,ISSN 0925-4005,https://doi.org/10.1016/j.snb.2017.04.182.

scholarly journals DEVELOPMENT OF A HIGH-CONTENT ANALYSIS PROCEDURE FOR QUANTITATIVE DETERMINATION OF PROAPOPTOTIC ABILITY OF SMALL MOLECULE COMPOUNDS

2020 ◽  
Vol 55 ◽  
pp. 58-61
Author(s):  
Angelina A. Romanova ◽  
◽  
Aleksandra Sagaidak ◽  
Milena Yu. Lvova ◽  
Tatiana A. Grigoreva ◽  
...  
Keyword(s):  
Cell Culture ◽  
Content Analysis ◽  
Imaging System ◽  
P53 Protein ◽  
Analysis Procedure ◽  
Protein Protein Interaction ◽  
High Content Analysis ◽  
Time Parameters ◽  
Human Colorectal Carcinoma

The article presents the development of conditions for cell experiments using the Operetta CLS™ imaging system. The significance of high-content analysis parameters is substantiated. The optimal concentration and time parameters for the treatment of the cell culture of human colorectal carcinoma HCT116 by MDM2-p53 protein-protein interaction inhibitors were selected. The effect of the studied substances on cell growth was analyzed using Operetta CLS™.

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