Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy (Conference Presentation)

2017 ◽  
Author(s):  
Jinho Kim ◽  
Beverley M. Henley ◽  
Charlene H. Kim ◽  
Henry A. Lester ◽  
Changhuei Yang
Keyword(s):  
Cell Culture ◽  
Imaging System

scholarly journals Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy

2016 ◽  
Vol 7 (8) ◽  
pp. 3097 ◽  
Author(s):  
Jinho Kim ◽  
Beverley M. Henley ◽  
Charlene H. Kim ◽  
Henry A. Lester ◽  
Changhuei Yang
Keyword(s):  
Cell Culture ◽  
Imaging System

scholarly journals An embedded application for cell culture confluency estimation using the InCellis® Smart Cell Imaging System

BioTechniques ◽  
2018 ◽  
Vol 64 (3) ◽  
pp. 127
Author(s):  
Laima Antanaviciute ◽  
Sophie Dubacq ◽  
Olivier Varet
Keyword(s):  
Cell Culture ◽  
Cell Imaging ◽  
Imaging System ◽  
Embedded Application

scholarly journals Incubator Embedded Cell Culture Imaging System (EmSight) Based on Fourier Ptychographic Microscopy

2017 ◽  
Author(s):  
Jinho Kim ◽  
Beverley M. Henley ◽  
Charlene H. Kim ◽  
Henry A. Lester ◽  
Changhuei Yang
Keyword(s):  
Cell Culture ◽  
Imaging System

scholarly journals A portable cell culture system with a simple microscope, oxygen sensor and MEA amplifier

2019 ◽  
Author(s):  
Dhanesh Kattipparambil Rajan
Keyword(s):  
Cell Culture ◽  
Chemical Sensing ◽  
Live Cell Imaging ◽  
Oxygen Sensor ◽  
Cell Imaging ◽  
Imaging System ◽  
Electrophysiological Recording

Cell culture in-vitro is a well-known method to develop cell and disease models for studying physiologically relevant mechanisms and responses for various applications in life sciences. Conventional methods for instance, using static culture flasks or well plates, have limitations, as these cannot provide accurate tractable models for advanced studies. However, microscale systems can overcome this since they mimic the cells' natural microenvironment adequately. We have developed a portable live-cell imaging system with an invert-upright-convertible architecture and a mini-bioreactor for long-term simultaneous cell imaging and analysis, chemical sensing and electrophysiological recording. Our system integrates biocompatible cell-friendly materials with modular measurement schemes and precise environment control and can be automated. High quality time-lapse cell imaging is hugely useful in cell/disease models. However, integration of advanced in-vitro systems into benchtop microscopes for in-situ imaging is tricky and challenging. This is especially true with device based biological systems, such as lab/organ/body-on-chips, or mini-bioreactors/microfluidic systems. They face issues ranging from optical and physical geometry incompatibilities to difficulties in connectivity of flow and perfusion systems. However, the novel modular system we have developed either as an inverted or as an upright system can easily accommodate virtually any in-vitro devices. Furthermore, it can accept additional sensor or measurement devices quite freely. Cell characterization, differentiation, chemical sensing, drug screening, microelectrode-array-electrophysiological recordings, and cell stimulation can be carried out with simultaneous in-situ imaging and analysis. Moreover, our system can be configured to capture images from regions that are otherwise inaccessible by conventional microscopes, for example, cells cultured on physical or biochemical sensor systems. We demonstrate the system for video-based beating analysis of cardiomyocytes, cell orientation analysis on nanocellulose, and simultaneous long-term in-situ microscopy with pO2 and temperature sensing. The compact microscope as such is comparable to standard phase-contrast-microscopes without any detectable aberrations and is useful practically for any in-situ microscopy demands. [1] D. K. Rajan et al., "A Portable Live-Cell Imaging System With an Invert-Upright-Convertible Architecture and a Mini-Bioreactorfor Long-Term Simultaneous Cell Imaging, Chemical Sensing, and Electrophysiological Recording," in IEEE Access, vol. 6,pp. 11063-11075, 2018. doi: 10.1109/ACCESS.2018.2804378. [2] Antti-Juhana Mäki et al., "A Portable Microscale Cell Culture System with Indirect Temperature Control"SLAS TECHNOLOGY: Translating Life Sciences Innovation 2018 23:6, 566-579. [3] Hannu Välimäki et al.,Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cellmodels,Sensors and Actuators B: Chemical,Volume 249,2017,pp.738-746,ISSN 0925-4005,https://doi.org/10.1016/j.snb.2017.04.182.

scholarly journals DEVELOPMENT OF A HIGH-CONTENT ANALYSIS PROCEDURE FOR QUANTITATIVE DETERMINATION OF PROAPOPTOTIC ABILITY OF SMALL MOLECULE COMPOUNDS

2020 ◽  
Vol 55 ◽  
pp. 58-61
Author(s):  
Angelina A. Romanova ◽  
◽  
Aleksandra Sagaidak ◽  
Milena Yu. Lvova ◽  
Tatiana A. Grigoreva ◽  
...  
Keyword(s):  
Cell Culture ◽  
Content Analysis ◽  
Imaging System ◽  
P53 Protein ◽  
Analysis Procedure ◽  
Protein Protein Interaction ◽  
High Content Analysis ◽  
Time Parameters ◽  
Human Colorectal Carcinoma

The article presents the development of conditions for cell experiments using the Operetta CLS™ imaging system. The significance of high-content analysis parameters is substantiated. The optimal concentration and time parameters for the treatment of the cell culture of human colorectal carcinoma HCT116 by MDM2-p53 protein-protein interaction inhibitors were selected. The effect of the studied substances on cell growth was analyzed using Operetta CLS™.

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

New Aspects in the Design of Electron Optical Magnification Systems

1972 ◽  
Vol 30 ◽  
pp. 606-607
Author(s):  
Willem H.J. Andersen
Keyword(s):  
Electron Microscope ◽  
Imaging System ◽  
Chromatic Aberration ◽  
Research Tool ◽  
Energy Losses ◽  
Objective Lens ◽  
Theoretical Limit ◽  
Optical Magnification ◽  
Chromatic Aberrations ◽  
High Degree

Electron microscope design, and particularly the design of the imaging system, has reached a high degree of perfection. Present objective lenses perform up to their theoretical limit, while the whole imaging system, consisting of three or four lenses, provides very wide ranges of magnification and diffraction camera length with virtually no distortion of the image. Evolution of the electron microscope in to a routine research tool in which objects of steadily increasing thickness are investigated, has made it necessary for the designer to pay special attention to the chromatic aberrations of the magnification system (as distinct from the chromatic aberration of the objective lens). These chromatic aberrations cause edge un-sharpness of the image due to electrons which have suffered energy losses in the object.There exist two kinds of chromatic aberration of the magnification system; the chromatic change of magnification, characterized by the coefficient Cm, and the chromatic change of rotation given by Cp.

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