Mean cell size and collagen orientation from 2D Fourier analysis on confocal laser scanning microscopy and two-photon fluorescence microscopy on human skin in vivo

2003 ◽  
Author(s):  
Gerald W. Lucassen ◽  
Bernard L. Bakker ◽  
Sieglinde Neerken ◽  
Rob F. M. Hendriks
Keyword(s):  
Fluorescence Microscopy ◽  
Fourier Analysis ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Collagen Orientation ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Two-Photon Microscopy Allows Imaging and Characterization of Cochlear Microvasculature In Vivo

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Friedrich Ihler ◽  
Mattis Bertlich ◽  
Bernhard Weiss ◽  
Steffen Dietzel ◽  
Martin Canis
Keyword(s):  
Fluorescence Microscopy ◽  
Inner Ear ◽  
Ex Vivo ◽  
Small Scale ◽  
Published Data ◽  
Cochlear Blood Flow ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Impairment of cochlear blood flow has been discussed as factor in the pathophysiology of various inner ear disorders. However, the microscopic study of cochlear microcirculation is limited due to small scale and anatomical constraints. Here, two-photon fluorescence microscopy is applied to visualize cochlear microvessels. Guinea pigs were injected with Fluorescein isothiocyanate- or Texas red-dextrane as plasma marker. Intravital microscopy was performed in four animals and explanted cochleae from four animals were studied. The vascular architecture of the cochlea was visualized up to a depth of90.0±22.7 μm. Imaging yielded a mean contrast-to-noise ratio (CNR) of3.3±1.7. Mean diameter in vivo was16.5±6.0 μm for arterioles and8.0±2.4 μm for capillaries. In explanted cochleae, the diameter of radiating arterioles and capillaries was measured with12.2±1.6 μm and6.6±1.0 μm, respectively. The difference between capillaries and arterioles was statistically significant in both experimental setups (P<0.001andP=0.022, two-way ANOVA). Measured vessel diameters in vivo and ex vivo were in agreement with published data. We conclude that two-photon fluorescence microscopy allows the investigation of cochlear microvessels and is potentially a valuable tool for inner ear research.

scholarly journals In Vivo Mammalian Brain Imaging Using One- and Two-Photon Fluorescence Microendoscopy

2004 ◽  
Vol 92 (5) ◽  
pp. 3121-3133 ◽  
Author(s):  
Juergen C. Jung ◽  
Amit D. Mehta ◽  
Emre Aksay ◽  
Raymond Stepnoski ◽  
Mark J. Schnitzer
Keyword(s):  
Laser Scanning ◽  
Frame Rate ◽  
Mammalian Brain ◽  
Cell Dynamics ◽  
Ca1 Neurons ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Rats And Mice ◽  
Photon Fluorescence

One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells in vivo. Both one- and two-photon fluorescence microendoscopy are based on compound gradient refractive index (GRIN) lenses that are 350–1,000 μm in diameter and provide micron-scale resolution. One-photon microendoscopy allows full-frame images to be viewed by eye or with a camera, and is well suited to fast frame-rate imaging. Two-photon microendoscopy is a laser-scanning modality that provides optical sectioning deep within tissue. Using in vivo microendoscopy we acquired video-rate movies of thalamic and CA1 hippocampal red blood cell dynamics and still-frame images of CA1 neurons and dendrites in anesthetized rats and mice. Microendoscopy will help meet the growing demand for in vivo cellular imaging created by the rapid emergence of new synthetic and genetically encoded fluorophores that can be used to label specific brain areas or cell classes.

scholarly journals Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

2020 ◽  
Vol 17 (3) ◽  
pp. 287-290 ◽  
Author(s):  
Jianglai Wu ◽  
Yajie Liang ◽  
Shuo Chen ◽  
Ching-Lung Hsu ◽  
Mariya Chavarha ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neural Activity ◽  
Microscopy Imaging ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Skeletal Muscle NAD(P)H Two-Photon Fluorescence Microscopy In Vivo: Topology and Optical Inner Filters

2005 ◽  
Vol 88 (3) ◽  
pp. 2165-2176 ◽  
Author(s):  
Emily C. Rothstein ◽  
Stefanie Carroll ◽  
Christian A. Combs ◽  
Paul D. Jobsis ◽  
Robert S. Balaban
Keyword(s):  
Skeletal Muscle ◽  
Fluorescence Microscopy ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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