High-order photobleaching in two-photon excitation fluorescence microscopy inside live cell

2003 ◽  
Author(s):  
Tongsheng Chen ◽  
Shaoqun Zeng ◽  
Qingming Luo ◽  
Wei Zhou ◽  
Zhihong Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
High Order ◽  
Live Cell ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy in living systems

1993 ◽  
Vol 51 ◽  
pp. 154-155 ◽  
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Singlet State ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two‐photon excitation fluorescence microscopy using a semiconductor laser

Bioimaging ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 70-75 ◽  
Author(s):  
Pekka E Hänninen ◽  
Martin Schrader ◽  
Erkki Soini ◽  
Stefan W Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Semiconductor Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Axial Resolution Enhancement by 4Pi Confocal Fluorescence Microscopy with Two-Photon Excitation

2007 ◽  
Vol 33 (5-6) ◽  
pp. 433-443 ◽  
Author(s):  
Sylvia Glaschick ◽  
Carlheinz Röcker ◽  
Karen Deuschle ◽  
Jörg Wiedenmann ◽  
Franz Oswald ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Resolution Enhancement ◽  
Confocal Fluorescence Microscopy ◽  
Axial Resolution ◽  
Two Photon ◽  
Photon Excitation ◽  
Confocal Fluorescence ◽  
Two Photon Excitation

Two-Photon Excitation Fluorescence Microscopy

2013 ◽  
pp. 2667-2671 ◽  
Author(s):  
Alberto Diaspro ◽  
Paolo Bianchini ◽  
Francesca Cella Zanacchi ◽  
Cesare Usai
Keyword(s):  
Fluorescence Microscopy ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-Photon Excitation Fluorescence Microscopy

2007 ◽  
pp. 751-789 ◽  
Author(s):  
Alberto Diaspro ◽  
Marc Schneider ◽  
Paolo Bianchini ◽  
Valentina Caorsi ◽  
Davide Mazza ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Principles of two-photon excitation fluorescence microscopy and other nonlinear imaging approaches

2006 ◽  
Vol 58 (7) ◽  
pp. 788-808 ◽  
Author(s):  
Martin Oheim ◽  
Darren J. Michael ◽  
Matthias Geisbauer ◽  
Dorte Madsen ◽  
Robert H. Chow
Keyword(s):  
Fluorescence Microscopy ◽  
Two Photon ◽  
Photon Excitation ◽  
Nonlinear Imaging ◽  
Two Photon Excitation

scholarly journals Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0147115 ◽  
Author(s):  
Rumelo Amor ◽  
Alison McDonald ◽  
Johanna Trägårdh ◽  
Gillian Robb ◽  
Louise Wilson ◽  
...  
Keyword(s):  
Time Resolution ◽  
Live Cell ◽  
High Time ◽  
High Time Resolution ◽  
Two Photon ◽  
Photon Excitation ◽  
Live Cell Microscopy ◽  
Cell Microscopy ◽  
Two Photon Excitation
Sign in / Sign up

Export Citation Format

Share Document